Alterations in rat brain proteins after desflurane anesthesia

Fütterer, Carsten D.; Maurer, Martin H.; Schmitt, Anne; Feldmann, Robert E.; Kuschinsky, Wolfgang; Waschke, Klaus F.

doi:10.1097/00000542-200402000-00019

Cited by 93Anesthesia and Neurotoxicity ResearchAnesthesia and Sedative AgentsIntensive Care Unit Cognitive Disorders

Alterations in rat brain proteins after desflurane anesthesia

Fütterer, Carsten D., Maurer, Martin H., Schmitt, Anne, Feldmann, Robert E., Kuschinsky, Wolfgang, Waschke, Klaus F.·2004·Anesthesiology

DOI 10.1097/00000542-200402000-00019

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Abstract

BACKGROUND: Volatile anesthetics disappear from an organism after the end of anesthesia. Whether changes of protein expression persist in the brain for a longer period is not known. This study investigates the question of whether the expression of proteins is altered in the rat brain after the end of desflurane anesthesia. METHODS: Three groups (n = 12 each) of rats were anesthetized with 5.7% desflurane in air for 3 h. Brains were removed directly after anesthesia, 24 h after anesthesia, or 72 h after anesthesia. Two additional groups (n = 12 each) served as naive conscious controls, in which the brains were removed without previous anesthesia 3 or 72 h after the start of the experiment. Cytosolic proteins were isolated. A proteome-wide study was performed, based on two-dimensional gel electrophoresis and mass spectrometry. RESULTS: Compared with conscious controls, significant (P < 0.05) increase/decrease was found: 3 h of anesthesia, 5/2 proteins; 24 h after anesthesia, 13/1 proteins; 72 h after anesthesia, 6/4 proteins. The overall changes in protein expression as quantified by the induction factor ranged from -1.67 (decrease to 60%) to 1.79 (increase by 79%) compared with the controls (100%). Some of these regulated proteins play a role in vesicle transport and metabolism. CONCLUSION: Desflurane anesthesia produces changes in cytosolic protein expression up to 72 h after anesthesia in the rat brain, indicating yet unknown persisting effects.

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Authors: Fütterer, C. D., Maurer, M. H., Schmitt, A., Feldmann, R. E., Kuschinsky, W., & Waschke, K. F.
Affiliations: Medizinische Fakultät Mannheim (DE)
Research Fellow.
Candidate of Medicine.
Heidelberg University (DE)
University of Mannheim (DE)
ORCID: Maurer, Martin H.
Feldmann, Robert E.
Venue: Anesthesiology
Publisher: Ovid Technologies (Wolters Kluwer Health)
Published: 2004-02-01
Volume: 100
Issue: 2
Pages: 302-308
ISSN: 0003-3022 (ISSN-L)
DOI: 10.1097/00000542-200402000-00019
OpenAlex: W2028200077
Cited by: 93
References: 39
Citations by year: 2026: 1 · 2025: 2 · 2024: 1 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 5 · 2018: 1 · 2017: 4 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 6 · 2012: 10
FWCI: 1.78
Type: journal article
Language: en
Open access: closed
Locations: Anesthesiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Neuroscience › Developmental Neuroscience › Anesthesia and Neurotoxicity Research
Health Sciences › Medicine › Anesthesiology and Pain Medicine › Anesthesia and Sedative Agents
Health Sciences › Medicine › Critical Care and Intensive Care Medicine › Intensive Care Unit Cognitive Disorders
Keywords: desflurane, volatile anesthetics, brain proteome, cytosolic proteins, protein expression changes, vesicle trafficking, brain metabolism, rat brain
MeSH terms: Desflurane, Anesthesia, Inhalation, Animals, Brain, Isoflurane, Male, Nerve Tissue Proteins, Rats, Wistar, Anesthetics, Inhalation, Proteome, Rats

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Alterations in rat brain proteins after desflurane anesthesia

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