The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Rapid Detection of Protein Aggregates in the Brains of Alzheimer Patients and Transgenic Mouse Models of Amyloidosis
Abstract
Extracellular and/or intracellular aggregates are pathological features of many, if not all, neurodegenerative diseases. In Alzheimer disease (AD), extracellular aggregates of beta-amyloid (Abeta) and intracellular aggregates of tau or a-synuclein are key diagnostic markers of the disease. We report here a method to rapidly detect these protein aggregates that relies on size exclusion filtration and immunostaining of trapped material, a method termed filter trapping. We demonstrate that aggregated forms of Abeta and tau are readily trapped in 0.22 microm cellulose acetate filter membranes, which can then be immunostained with specific antibodies in a manner similar to the standard immunoblot. Coupling this method with serial dilution permits a rapid assessment of relative aggregate burden.
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Filter-trap assay showed that [APPswe/PS1dE9](+/−)/RAP(+/−) mice had about twice as much high-molecular-weight Aβ as controls.
“we used a filter-trap assay [32] to demonstrate that the brains of mice expressing APPswe/PS1dE9 with a partial loss of RAP contained about twice as much high-molecular-weight Aβ as controls (Fig. 2).”
From Receptor-Associated Protein (RAP) Plays a Central Role in Modulating Aβ Deposition in APP/PS1 Transgenic Mice (2008) · cites this paper as [32]
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Scherzinger, E., Lurz, R., Turmaine, M., Mangiarini, L., Hollenbach, B., Hasenbank, R., Bates, G. P., Davies, S. W., Lehrach, H., & Wanker, E. E. (1997) · Cell · doi:10.1016/s0092-8674(00)80514-0 · Cited by 1,291 · cited 2×
“From studies of Huntington disease, an alternative approach to detecting aggregated proteins has emerged whereby tissue samples are filtered through cellulose acetate membranes (Scherzinger et al., 1997; Scherzinger et al., 1999).”
BackgroundBorchelt, D. R., Ratovitski, T., van Lare, J., Lee, M. K., Gonzales, V., Jenkins, N. A., Copeland, N. G., Price, D. L., & Sisodia, S. S. (1997) · Neuron · doi:10.1016/s0896-6273(00)80974-5 · Cited by 1,023
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Research works printed but not linked: 11
- Borchelt, 1996a, Genet Anal Biomed Eng, 13, 159, 10.1016/S1050-3862(96)00167-2 · DOI · Paper page
- Borchelt, 2001, Neurobiol Aging, 23, 171, 10.1016/S0197-4580(01)00280-9 · DOI
- Borchelt, 1996b, Neuron, 17, 1005, 10.1016/S0896-6273(00)80230-5 · DOI · Paper page
- Gundersen, 1988, APMIS, 96, 857, 10.1111/j.1699-0463.1988.tb00954.x · DOI
- Jankowsky, 2001, Biomol Eng, 17, 157, 10.1016/S1389-0344(01)00067-3 · DOI · Paper page
- Laemmli, 1970, Nature, 227, 680, 10.1038/227680a0 · DOI · Paper page
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- Price, 1998, Science, 282, 1079, 10.1126/science.282.5391.1079 · DOI
- Scherzinger, 1999, Proc Natl Acad Sci U S A, 96, 4604, 10.1073/pnas.96.8.4604 · DOI
- Slunt, 1994, J Biol Chem, 269, 2637, 10.1016/S0021-9258(17)41992-2 · DOI
- Terry, 1983, Ann Neurol, 14, 497, 10.1002/ana.410140502 · DOI
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Mackenzie, I. R. A., Frick, P., Grässer, F. A., Gendron, T. F., Petrucelli, L., Cashman, N. R., Edbauer, D., Kremmer, E., Prudlo, J., Troost, D., et al. (2015) · Acta Neuropathologica · doi:10.1007/s00401-015-1476-2 · Cited by 231
Jankowsky, J. L., Slunt, H. H., Gonzales, V., Savonenko, A. V., Wen, J. C., Jenkins, N. A., Copeland, N. G., Younkin, L. H., Lester, H. A., Younkin, S. G., et al. (2005) · PLoS Medicine · doi:10.1371/journal.pmed.0020355 · Cited by 237
Self-citationBrelstaff, J. H., Mason, M., Katsinelos, T., McEwan, W. A., Ghetti, B., Tolkovsky, A. M., & Spillantini, M. G. (2021) · Science Advances · doi:10.1126/sciadv.abg4980 · Cited by 135
Defining α-synuclein species responsible for Parkinson’s disease phenotypes in mice
Froula, J. M., Castellana-Cruz, M., Anabtawi, N. M., Camino, J. D., Chen, S. W., Thrasher, D. R., Freire, J., Yazdi, A. A., Fleming, S., Dobson, C. M., et al. (2019) · Journal of Biological Chemistry · doi:10.1074/jbc.ra119.007743 · Cited by 129
Rodent Aβ Modulates the Solubility and Distribution of Amyloid Deposits in Transgenic Mice
Jankowsky, J. L., Younkin, L. H., Gonzales, V., Fadale, D. J., Slunt, H. H., Lester, H. A., Younkin, S. G., & Borchelt, D. R. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m611050200 · Cited by 122
Self-citationXu, G., Karch, C., Li, N., Lin, N., Fromholt, D., Gonzales, V., & Borchelt, D. R. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003159 · Cited by 14
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Metadata
- Authors
- Xu, G., Gonzales, V., & Borchelt, D. R.
- Venue
- Alzheimer Disease & Associated Disorders
- Published
- 2002-07
- Volume
- 16
- Issue
- 3
- Pages
- 191-195
- ISSN
- 0893-0341
- Cited by
- 37
- References
- 2
- Language
- en
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