The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Induction of apoptosis and/or necrosis following exposure to antitumour agents in a melanoma cell line, probably through modulation of Bcl-2 family proteins
Abstract
Malignant melanoma cells have been reported to be highly resistant to chemotherapeutic agents. To gain insight into the molecular mechanisms underlying chemotherapeutic drug resistance, we examined the role of the Bcl-2 family members Bcl-2 and Bax in cell death in the melanoma cell line G361 following stimulation with cisplatin (CDDP) or dacarbazine (DTIC). Trypan blue dye exclusion showed that both CDDP and DTIC induced death of G361 cells. Apoptotic and necrotic cell death could be distinguished by flow cytometry using combined staining with annexin V and 7-amino-actinomycin D (7-AAD). CDDP-induced cell death at a low concentration (0.6 micro g/ml) was mainly due to apoptosis (annexin V+/7-AAD-), while a mixture of apoptosis and secondary necrosis (annexin V+/7-AAD+) was found at a high concentration (6 micro g/ml). DTIC at the concentrations used induced only apoptosis. CDDP-induced apoptosis and secondary necrosis were accompanied by activation of caspase-3 and modulation of Bcl-2 family members Bcl-2 and Bax. On Western blotting Bax was seen to be upregulated with concomitant downregulation of Bcl-2. Flow cytometry, which enables measurement of protein at the single-cell level, revealed that Bcl-2+/Bax- cells were decreased, with a slight concomitant rise in Bcl-2-/Bax+ cells on stimulation with CDDP. These findings suggest that the chemotherapeutic agents CDDP and DTIC induce apoptosis and/or secondary necrosis depending on dose, probably involving the modulation of Bcl-2 family proteins.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Both DTIC and Temozolomide act through a common degradation product (MTIC).”
From Chemotherapeutic treatment efficacy and sensitivity are increased by adjuvant alternating electric fields (TTFields) (2009) · cites this paper as [16]
Citations
Cites
Apoptosis in the Pathogenesis and Treatment of Disease
Thompson, C. B. (1995) · Science · doi:10.1126/science.7878464 · Cited by 6,524
Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programed cell death
Oltval, Z. N., Milliman, C. L., & Korsmeyer, S. J. (1993) · Cell · doi:10.1016/0092-8674(93)90509-o · Cited by 6,003
The TNF and TNF Receptor Superfamilies
Locksley, R. M., Killeen, N., & Lenardo, M. J. (2001) · Cell · doi:10.1016/s0092-8674(01)00237-9 · Cited by 3,650
Koopman, G., Reutelingsperger, C. P. M., Kuijten, G., Keehnen, R. M. J., Pals, S. T., & van Oers, M. H. J. (1994) · Blood · doi:10.1182/blood.v84.5.1415.1415 · Cited by 2,687
Social controls on cell survival and cell death
RAFF, M. C. (1992) · Nature · doi:10.1038/356397a0 · Cited by 2,591
A Combinatorial Approach Defines Specificities of Members of the Caspase Family and Granzyme B
Thornberry, N. A., Rano, T. A., Peterson, E. P., Rasper, D., Timkey, T., Garcia-Calvo, M. M., Houtzager, V. M., Nordstrom, P. A., Roy, S., Vaillancourt, J. P., et al. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.29.17907 · Cited by 2,089
Koopman, G., Reutelingsperger, C., Kuijten, G., et al. (1994) · Blood · doi:10.1182/blood.v84.5.1415.bloodjournal8451415 · Cited by 2,068
The proto-oncogene Bcl-2 and its role in regulating apoptosis
Kroemer, G. (1997) · Nature Medicine · doi:10.1038/nm0697-614 · Cited by 1,739
Natural adjuvants: Endogenous activators of dendritic cells
Gallucci, S., Lolkema, M. P., & Matzinger, P. (1999) · Nature Medicine · doi:10.1038/15200 · Cited by 1,601
Intracellular ATP levels determine cell death fate by apoptosis or necrosis.
Eguchi, Y., Shimizu, S., & Tsujimoto, Y. (1997) · PubMed · Cited by 1,249
Flagged first, then by citations
Research works printed but not linked: 5
- Di Felice, 1998, Int J Oncol, 13, 225
- Kim, 1999, Int J Oncol, 15, 751
- Miyashita, 1992, Cancer Res, 52, 5407
- Searle, 1982, Pathol Annu, 17, 229
- Eguchi, 1997, Cancer Res, 57, 1835
Cited by
Sorted by citations
New insights into the mechanism of aminoglycoside nephrotoxicity: an integrative point of view
López-Novoa, J. M., Quirós, Y., Vicente-Vicente, L., Morales, A. I., & López-Hernández, F. J. (2010) · Kidney International · doi:10.1038/ki.2010.337 · Cited by 756
Kirson, E. D., Schneiderman, R. S., Dbalý, V., Tovaryš, F., Vymazal, J., Itzhaki, A., Mordechovich, D., Gurvich, Z., Shmueli, E., Goldsher, D., et al. (2009) · BMC Medical Physics · doi:10.1186/1756-6649-9-1 · Cited by 224
AMPK‐mediated autophagy inhibits apoptosis in cisplatin‐treated tumour cells
Harhaji-Trajkovic, L. M., Vilimanovich, U., Kravic‐Stevovic, T., Bumbaširević, V., & Trajković, V. (2009) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2009.00663.x · Cited by 189
Yang, W., Vethanayagam, R. R., Dong, Y., Cai, Q., & Hu, B. H. (2015) · Neuroscience · doi:10.1016/j.neuroscience.2015.05.081 · Cited by 115
Shi, Y., Zhai, H., Wang, X., Han, Z., Liu, C., Lan, M., Du, J.-P., Guo, C., Zhang, Y., Wu, K., et al. (2004) · Experimental Cell Research · doi:10.1016/j.yexcr.2004.02.009 · Cited by 111
Metadata
- Authors
- Shibuya, H., Kato, Y., Saito, M., Isobe, T., Tsuboi, R., Koga, M., Toyota, H., & Mizuguchi, J.
- Affiliations
- Tokyo Medical University (JP)
- Tokyo Medical University Hospital (JP)
- Corresponding author
- Mizuguchi, Junichiro
- Venue
- Melanoma Research
- Published
- 2003-10
- Volume
- 13
- Issue
- 5
- Pages
- 457-464
- ISSN
- 0960-8931 (ISSN-L)
- OpenAlex
- W2000352929
- Cited by
- 46
- References
- 39
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 2 · 2022: 1 · 2021: 2 · 2019: 1 · 2017: 1 · 2015: 2 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 0.92
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Melanoma Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Keywords
- melanoma, apoptosis, secondary necrosis, Bcl-2 family proteins, Bcl-2, Bax, cisplatin, dacarbazine, chemoresistance, caspase-3, dose-dependent cell death
- MeSH terms
- Antineoplastic Agents, Cell Survival, Cisplatin, Dacarbazine, Dactinomycin, Dose-Response Relationship, Drug, Coloring Agents, Enzyme Activation, Flow Cytometry, Fluorescent Dyes, Humans, Melanoma, Necrosis, Time Factors, Trypan Blue, Blotting, Western, Down-Regulation, Up-Regulation, Cell Death, Apoptosis, Annexin A5, Antineoplastic Agents, Alkylating, Proto-Oncogene Proteins c-bcl-2, Caspases, Cell Line, Tumor, Caspase 3
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
