Rat Blood–Brain Barrier Genomics. II

Li, Jian Yi; Boado, Ruben J.; Pardridge, William M.

doi:10.1097/01.wcb.0000040944.89393.0f

Cited by 46Barrier Structure and Function StudiesDrug Transport and Resistance MechanismsCerebrovascular and genetic disorders

Rat Blood–Brain Barrier Genomics. II

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Li, Jian Yi, Boado, Ruben J., Pardridge, William M.·2002·Journal of Cerebral Blood Flow & Metabolism

DOI 10.1097/01.wcb.0000040944.89393.0f

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Abstract

The tissue-specific gene expression at the brain microvasculature, which forms the blood–brain barrier (BBB) in vivo, can be elucidated with a brain vascular genomics program, which starts with the isolation of gene products derived from purified brain microvessels. Genes commonly expressed in peripheral organs are subtracted with the suppression subtractive hybridization method using driver cDNA produced from a pool of rat liver/kidney–derived poly A+RNA. From a screen of 480 clones in the subtracted tester cDNA library, 156 clones were sequenced. The clones fell into 3 groups: known genes (51%), rat expressed sequence tags (31%), and novel rat genes not found in databases (18%). The known genes could be categorized into families of common function including vascular remodeling, signal transduction, transcription factors, biologic transport, vascular amyloid, hemostasis, myelin, lipids, secretion, cytoskeleton, and junctional complexes. Brain vascular genomics, or BBB genomics, allows for an accelerated discovery of the gene families that are differentially expressed at the microvasculature in brain.

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Pericytes, SMCs, and endothelial cells express thousands of transcripts encoding different transporters, receptors, active efflux pumps, ion channels, and regulatory molecules, whose expression pattern varies by zonation along the arteriocapillary-venous axis and cell type.
“Pericytes, SMCs, and endothelial cells express thousands of transcripts encoding different transporters, receptors, active efflux pumps, ion channels, and regulatory molecules (28, 72, 86, 129, 242, 358, 359, 610, 675, 678), whose expression pattern varies by zonation along the arteriocapillary-venous axis and cell type (610).”
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Authors: Li, J. Y., Boado, R. J., & Pardridge, W. M.
Affiliations: University of California, Los Angeles (US)
Department of Medicine, UCLA School of Medicine, Los Angeles, California, U.S.A.
ORCID: Pardridge, William M.
Venue: Journal of Cerebral Blood Flow & Metabolism
Publisher: SAGE Publications
Published: 2002-11
Volume: 22
Issue: 11
Pages: 1319-1326
ISSN: 0271-678X (ISSN-L)
1559-7016
DOI: 10.1097/01.wcb.0000040944.89393.0f
OpenAlex: W1976177535
Cited by: 46
References: 51
Citations by year: 2026: 1 · 2025: 3 · 2024: 3 · 2022: 3 · 2020: 2 · 2019: 1 · 2018: 2 · 2015: 3 · 2014: 1 · 2013: 2 · 2012: 1
FWCI: 2.14
Type: journal article
Language: en
Open access: bronze
doi.org
Licence: journals.sagepub.com/page/policies/text-and-data-mining-license (tdm)
Locations: Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
National Institute of Mental Health (R01MH061138, MH61138)
Topics: Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Health Sciences › Medicine › Neurology › Cerebrovascular and genetic disorders
Keywords: blood-brain barrier, cerebral microvasculature, gene expression profiling, suppression subtractive hybridization, expressed sequence tags, vascular remodeling, signal transduction, transcription factors, hemostasis, myelin, vascular amyloid, cytoskeleton, junctional complexes, rats
MeSH terms: Animals, Blood-Brain Barrier, Male, Gene Expression, Rats, Sprague-Dawley, Expressed Sequence Tags, Oligonucleotide Array Sequence Analysis, Genomics, Rats

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Rat Blood–Brain Barrier Genomics. II

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