Phenotype and Genotype of Pancreatic Cancer Cell Lines
Open accessAbstract
The dismal prognosis of pancreatic adenocarcinoma is due in part to a lack of molecular information regarding disease development. Established cell lines remain a useful tool for investigating these molecular events. Here we present a review of available information on commonly used pancreatic adenocarcinoma cell lines as a resource to help investigators select the cell lines most appropriate for their particular research needs. Information on clinical history; in vitro and in vivo growth characteristics; phenotypic characteristics, such as adhesion, invasion, migration, and tumorigenesis; and genotypic status of commonly altered genes (KRAS, p53, p16, and SMAD4) was evaluated. Identification of both consensus and discrepant information in the literature suggests careful evaluation before selection of cell lines and attention be given to cell line authentication.
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Discrepancies in mutational status of TP53 and CDKN2A/p16 among cell lines caution researchers about discrepancies between laboratory-specific and literature-reported mutational statuses.
“Discrepancies in the mutational status of TP53 and CDKN2A/p16 among cell lines caution researchers about discrepancies between laboratory-specific and literature-reported mutational statuses [20].”
From Significance of TP53, CDKN2A, SMAD4 and KRAS in Pancreatic Cancer (2024) · cites this paper as [20]
The MiaPaca2 cell line was initially generated from a primary PDAC lesion of a 65-year-old male with tumor involving pancreatic body, tail, and periaortic invasion.
“The MiaPaca2 cell line was initially generated from a primary PDAC lesion of a 65-year-old male, where the tumor had involved the pancreatic body, tail, and demonstrated periaortic invasion24.”
From Occult polyclonality of preclinical pancreatic cancer models drives in vitro evolution (2022) · cites this paper as [24]
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- Authors
- Deer, E. L., González-Hernández, J., Coursen, J. D., Shea, J. E., Ngatia, J., Scaife, C. L., Firpo, M. A., & Mulvihill, S. J.
- Affiliations
- University of Utah (US)
- Venue
- Pancreas
- Published
- 2010-05
- Volume
- 39
- Issue
- 4
- Pages
- 425-435
- ISSN
- 0885-3177 (ISSN-L)
- PMC
- PMC2860631
- OpenAlex
- W2168992025
- Cited by
- 936
- References
- 125
- Citations by year
- 2026: 34 · 2025: 50 · 2024: 67 · 2023: 67 · 2022: 83 · 2021: 84 · 2020: 84 · 2019: 84 · 2018: 77 · 2017: 79 · 2016: 63 · 2015: 39 · 2014: 58 · 2013: 23 · 2012: 29
- FWCI
- 2.34
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Pancreas (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute (T35 HL 07744, T35HL007744)
- Topics
- Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer Genomics and Diagnostics
- Health Sciences › Medicine › Surgery › Pancreatitis Pathology and Treatment
- Keywords
- pancreatic ductal adenocarcinoma, pancreatic cancer cell lines, cell line selection, cell line authentication, cell adhesion, cell invasion, cell migration, tumorigenicity, KRAS mutation, p53, p16, SMAD4, molecular alterations
- MeSH terms
- Genotype, Humans, Pancreatic Neoplasms, Phenotype, Proto-Oncogene Proteins, Tumor Suppressor Protein p53, Proto-Oncogene Proteins p21(ras), ras Proteins, Cyclin-Dependent Kinase Inhibitor p16, Cell Line, Tumor, Smad4 Protein
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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