Laparoscopic Diagnostics of Acute Bowel Ischemia Using Ultraviolet Light and Fluorescein Dye: An Experimental Study
Abstract
Purpose The aim of the study was to show the diagnostic potential of laparoscopy using fluorescein dye and ultraviolet light in acute bowel ischemia. Materials and methods The study involved 12 domestic pigs. Under general anesthesia, the peripheral branch of the superior mesenteric artery was embolized using polyvinyl-alcohol microparticles. Two hours after the embolization, optical filters were placed into the laparoscopic set to produce ultraviolet light. Fluorescein dye was given intravenously, and the bowel was inspected. Clips were placed on the border of the ischemia that was visualized with fluorescein. Resection of the ischemic part of the bowel and anastomosis of the viable parts were carried out using laparoscopic linear cutting staplers. After 24 hours, a laparoscopic second-look procedure was carried out to verify the viability of the anastomosis. Results The method was in all cases able to recognize intestinal ischemia and reliably differentiate ischemic bowel segments from viable bowel. Microscopic analysis of the ischemic specimens showed beginning ischemic changes of the bowel tissues. Conclusions The method should be considered a valuable diagnostic procedure both for diagnostics of early stage of acute bowel ischemia and for second-look procedures.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Flowmetry with fluorescein dye is currently part of the accepted standard of clinical care for intraoperative assessment of bowel viability.
“Flowmetry with fluorescein dye is currently part of the accepted standard of clinical care for intraoperative assessment of bowel viability.”
From Acute mesenteric ischemia: updated guidelines of the World Society of Emergency Surgery. (2022) · cites this paper as [88]
Cites
Shackelford's Surgery of the Alimentary Tract
Yeo, C. J. (1991) · The Medicine Forum · Cited by 243 · see citing works
BULKLEY, G. B., ZUIDEMA, G. D., HAMILTON, S. R., OʼMARA, C. S., KLACSMANN, P. G., & HORN, S. D. (1981) · Annals of Surgery · doi:10.1097/00000658-198105000-00014 · Cited by 203 · see citing works
Current Surgical Diagnosis and Treatment
(1984) · Critical Care Medicine · doi:10.1097/00003246-198404000-00018 · Cited by 166 · see citing works
Laparoscopic compared with conventional treatment of acute adhesive small bowel obstruction
Wullstein, C., & Gross, E. (2003) · British journal of surgery · doi:10.1002/bjs.4177 · Cited by 165 · see citing works
Aggressive Approach to Acute Mesenteric Ischemia
Kaleya, R. N., Sammartano, R. J., & Boley, S. J. (1992) · Surgical Clinics of North America · doi:10.1016/s0039-6109(16)45633-1 · Cited by 152 · see citing works
Shackelford's Surgery of the Alimentary Tract
Hermann, R. E. (1991) · JAMA · doi:10.1001/jama.1991.03470110122050 · Cited by 136 · see citing works
Diagnostic Tests for Intestinal Ischemia
Kurland, B., Brandt, L. J., & Delany, H. M. (1992) · Surgical Clinics of North America · doi:10.1016/s0039-6109(16)45629-x · Cited by 119 · see citing works
Operative Assessment of Intestinal Viability
Horgan, P. G., & Gorey, T. F. (1992) · Surgical Clinics of North America · doi:10.1016/s0039-6109(16)45632-x · Cited by 117 · see citing works
Acosta, S., Nilsson, T. K., & Björck, M. (2004) · British journal of surgery · doi:10.1002/bjs.4645 · Cited by 115 · see citing works
Preliminary study of D-dimer as a possible marker of acute bowel ischaemia
Acosta, S., Nilsson, T. K., & Björck, M. (2001) · British journal of surgery · doi:10.1046/j.1365-2168.2001.01711.x · Cited by 93 · see citing works
Qualitative and quantitative fluorescein fluorescence in determining intestinal viability
Carter, M. S., Fantinl, G. A., Sammartano, R. J., Mttsudo, S., Silverman, D. G., & Boley, S. J. (1984) · The American Journal of Surgery · doi:10.1016/0002-9610(84)90044-8 · Cited by 61 · see citing works
Current Surgical Diagnosis and Treatment
BROOKS, J. R. (1980) · Annals of Surgery · doi:10.1097/00000658-198008000-00032 · Cited by 57 · see citing works
Bedside diagnostic minilaparoscopy in the intensive care patient
Gagné, D. J., Malay, M. B., Hogle, N. J., & Fowler, D. L. (2002) · Surgery · doi:10.1067/msy.2002.122607 · Cited by 57 · see citing works
Laparoscopy in the critically ill
Orlando, R., & Crowell, K. L. (1997) · Surgical Endoscopy · doi:10.1007/s004649900532 · Cited by 52 · see citing works
Doppler Ultrasound, Laser Doppler, and Perfusion Fluorometry in Bowel Ischemia
Lynch, T. G. (1988) · Archives of Surgery · doi:10.1001/archsurg.1988.01400280093017 · Cited by 51 · see citing works
Agresta, F., Michelet, I., Coluci, G., & Bedin, N. (2000) · Surgical Endoscopy · doi:10.1007/s004640000022 · Cited by 41 · see citing works
Diagnostic laparoscopy in mesenteric ischemia
Zamir, G. A., & Reissman, P. (1998) · Surgical Endoscopy · doi:10.1007/s004649900688 · Cited by 36 · see citing works
D-Dimer in the Early Diagnosis of Acute Mesenteric Ischemia Secondary to Arterial Occlusion in Rats
Kurt, Y. G., Akın, M., Demirbas, S., Uluutku, H., Gulderen, M., Avşar, K., & ÇELENK, T. (2005) · European Surgical Research · doi:10.1159/000087866 · Cited by 36 · see citing works
The routine use of diagnostic laparoscopy in the intensive care unit
Pecoraro, A. M., Cacchione, R. N., Sayad, P., Williams, M., & FERZLI, G. S. (2001) · Surgical Endoscopy · doi:10.1007/s004640000371 · Cited by 36 · see citing works
The Use of Infrared Photoplethysmography in Identifying Early Intestinal Ischemia
Pearce, W. H. (1987) · Archives of Surgery · doi:10.1001/archsurg.1987.01400150062012 · Cited by 35 · see citing works
ABDOMINAL VASCULAR CATASTROPHES
Eldrup-Jørgensen, J., Hawkins, R. E., & Bredenberg, C. E. (1997) · Surgical Clinics of North America · doi:10.1016/s0039-6109(05)70619-8 · Cited by 35 · see citing works
Right hemicolectomy with a linear cutting stapler
Meagher, A. P., & Wolff, B. G. (1994) · Diseases of the Colon & Rectum · doi:10.1007/bf02049322 · Cited by 32 · see citing works
Intraoperative Evaluation of Intestinal Ischemia
Dyess, D. L., BRUNER, B. W., Donnell, C. A., Ferrara, J. J., & Powell, R. W. (1991) · Southern Medical Journal · doi:10.1097/00007611-199108000-00008 · Cited by 31 · see citing works
NEW METHODS FOR DETERMINING THE VIABILITY OF BOWEL
Herrlin, J. (1942) · Archives of Surgery · doi:10.1001/archsurg.1942.01220050096008 · Cited by 28 · see citing works
[Mesenterial infarct. New aspects of diagnosis and therapy].
Paes, E. H. J., Jf, V., Hutschenreiter, S., Mh, S., Kübel, R., & Schölzel, E. (1988) · PubMed · Cited by 27 · see citing works
The recovery of intestine after ischaemic injury
GOREY, T. F. (1980) · British journal of surgery · doi:10.1002/bjs.1800671006 · Cited by 25 · see citing works
Laparoscopy-assisted small bowel resection.
Ehrmantraut, W., & Sardi, A. (1997) · PubMed · Cited by 25 · see citing works
Simplified Laparoscopic Approach to “Second-Look” Laparotomy: A Review
Seshadri, P. A., Poulin, E. C., Mamazza, J., & Schlachta, C. M. (1999) · Surgical Laparoscopy Endoscopy & Percutaneous Techniques · doi:10.1097/00129689-199908000-00012 · Cited by 24 · see citing works
McGinty, J. J., Hogle, N., & Fowler, D. L. (2003) · Surgical Endoscopy And Other Interventional Techniques · doi:10.1007/s00464-001-8255-y · Cited by 18 · see citing works
The laparoscopic second look for ischemic bowel disease
SLUTZKI, S., Halpern, Z., Negri, M., Kais, H., & Halevy, A. (1996) · Surgical Endoscopy · doi:10.1007/bf00193045 · Cited by 17 · see citing works
Fluorescein-assisted laparoscopy in the identification of arterial mesenteric ischemia
Kam, D., & Scheeres, D. E. (1993) · Surgical Endoscopy · doi:10.1007/bf00704381 · Cited by 16 · see citing works
Laparoscopic procedures for small bowel disease
Duh, Q. (1993) · Baillière s Clinical Gastroenterology · doi:10.1016/0950-3528(93)90018-n · Cited by 13 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Acute mesenteric ischemia: updated guidelines of the World Society of Emergency Surgery.
Bala, M., Catena, F., Kashuk, J., De Simone, B., Gomes, C. A., Weber, D., Sartelli, M., Coccolini, F., Kluger, Y., Abu-Zidan, F. M., et al. (2022) · World Journal of Emergency Surgery · doi:10.1186/s13017-022-00443-x · Cited by 380 · see citing works
“Fluorescein can therefore be used to visualize perfusion in open laparotomies using a Woods Lamp or laparoscopically using an endoscope with appropriate filters [88, 89].”
MethodPorcine models of digestive disease: the future of large animal translational research
Gonzalez, L. M., Moeser, A. J., & Blikslager, A. T. (2015) · Translational research · doi:10.1016/j.trsl.2015.01.004 · Cited by 279 · see citing works
Acute Mesenteric Ischemia: Diagnostic Approach and Surgical Treatment
Wyers, M. C. (2010) · Seminars in Vascular Surgery · doi:10.1053/j.semvascsurg.2009.12.002 · Cited by 259 · see citing works
Agresta, F., Ansaloni, L., Baiocchi, G. L., Bergamini, C., Campanile, F. C., Carlucci, M., Cocorullo, G., Corradi, A., Franzato, B., Lupo, M., et al. (2012) · Surgical Endoscopy · doi:10.1007/s00464-012-2331-3 · Cited by 215 · see citing works
Current Management of Small Bowel Obstruction
Zielinski, M. D., & Bannon, M. P. (2011) · Advances in Surgery · doi:10.1016/j.yasu.2011.03.017 · Cited by 108 · see citing works
Acute mesenteric ischemia after cardio-pulmonary bypass surgery
Abboud, B., Daher, R., & Jaoude, J. B. (2008) · World Journal of Gastroenterology · doi:10.3748/wjg.14.5361 · Cited by 69 · see citing works
Techniques for intraoperative evaluation of bowel viability in mesenteric ischemia: A review
Bryski, M. G., Sulyok, L. G. F., Kaplan, L. J., Singhal, S. K., & Keating, J. J. (2020) · The American Journal of Surgery · doi:10.1016/j.amjsurg.2020.01.042 · Cited by 50 · see citing works
Alemanno, G., Somigli, R., Prosperi, P., Bergamini, C., Maltinti, G., Giordano, A. B. F., & Valeri, A. (2016) · International Journal of Surgery Case Reports · doi:10.1016/j.ijscr.2016.07.016 · Cited by 35 · see citing works
Tshomba, Y., Coppi, G., Marone, E. M., Bertoglio, L., Kahlberg, A., Carlucci, M., & Chiesa, R. (2012) · European Journal of Vascular and Endovascular Surgery · doi:10.1016/j.ejvs.2012.03.017 · Cited by 32 · see citing works
Necrotizing enterocolitis: Early conventional and fluorescein laparoscopic assessment
Numanoğlu, A., & Millar, A. J. W. (2011) · Journal of Pediatric Surgery · doi:10.1016/j.jpedsurg.2010.11.021 · Cited by 30 · see citing works
The Role of Laparoscopy in Emergency Abdominal Surgery
Cocorullo, G., Gulotta, G., Mirabella, A., & Mandalà, V. (2011) · Updates in surgery/Updates in surgery series · doi:10.1007/978-88-470-2327-7 · Cited by 20 · see citing works
Sinz, S., Schneider, M. A., Graber, S., Alkadhi, H., Rickenbacher, A., & Turina, M. (2022) · Surgical Endoscopy · doi:10.1007/s00464-022-09673-1 · Cited by 17 · see citing works
Verhaar, N., & Geburek, F. (2025) · Veterinary Surgery · doi:10.1111/vsu.14248 · Cited by 10 · see citing works
Ritz, J.-P., & Buhr, H. J. (2011) · Der Chirurg · doi:10.1007/s00104-011-2098-4 · Cited by 10 · see citing works
Agresta, F., Campanile, F. C., Anania, G., & Bergamini, C. (2016) · Springer eBooks · doi:10.1007/978-3-319-29620-3 · Cited by 8 · see citing works
Yu, W., Liang, Y., Gao, J., Xie, D., & Xiong, J. (2024) · Journal of Cardiothoracic Surgery · doi:10.1186/s13019-024-02790-z · Cited by 7 · see citing works
Dual energy CT in necrotizing enterocolitis; a novel diagnostic approach
Özgür, Ö., Cesur, E., Sade, R., Fırıncı, B., Kara, M., Çelikkaya, M. E., Oral, A., Yiğiter, M., & Özmen, S. (2021) · TURKISH JOURNAL OF MEDICAL SCIENCES · doi:10.3906/sag-2103-294 · Cited by 7 · see citing works
Wyers, M. C. (2010) · Elsevier eBooks · doi:10.1016/b978-1-4160-5223-4.00149-9 · Cited by 4 · see citing works
Update in Laparoscopic Approach to Acute Mesenteric Ischemia
Cocorullo, G., Falco, N., Fontana, T., Tutino, R., Salamone, G., & Gulotta, G. (2016) · Springer eBooks · doi:10.1007/978-3-319-29620-3_13 · Cited by 2 · see citing works
Fluorescent test accurately predicts leak of ischemic colon anastomosis in rats
Moschetti, L., Ghezzi, T. L., Müller, B. G., Dias, A. B., & Corleta, O. C. (2017) · Acta Cirúrgica Brasileira · doi:10.1590/s0102-865020170060000004 · Cited by 2 · see citing works
Laparoscopy in Acute Mesenteric Ischemia
Cocorullo, G., Mirabella, A., Gulotta, G., & Mandalà, V. (2011) · Updates in surgery/Updates in surgery series · doi:10.1007/978-88-470-2327-7_9 · Cited by 1 · see citing works
Laparoscopic Approach to Acute Mesenteric Ischemia in Elderly Patients
Milone, M., D’Amore, A., Manigrasso, M., & Amato, B. (2021) · Springer eBooks · doi:10.1007/978-3-030-79990-8_21
(2020) · Thieme eBooks · doi:10.1055/b-0040-178613
Pérez-Benítez, O. A., Pérez-Cantú, A., & Ángeles, D. D. L. (2021) · Revista Mexicana de Cirugía Endoscópica · doi:10.35366/106480
Islam, S., Maughn, A., Payne, O., Bheem, V., & Harnarayan, P. (2023) · Journal of Case Reports · doi:10.17659/01.2023.0014
Acute Non-Occlusive Mesenteric Ischemia
Timerbulatov, S. V., Радиковна, Г. А., Плечев, В. В., Garaev, R. R., & Timerbulatov, V. M. (2025) · Creative Surgery and Oncology · doi:10.24060/2076-3093-2025-15-4-390-401
Technique of Open Mesenteric Catheter Embolectomy
Erben, Y., & Kalra, M. (2014) · Springer eBooks · doi:10.1007/978-1-4939-1847-8_16
- Authors
- Jiri, P., Alexander, F., Michal, P., Jan, R., Dimitar, H.-N., David, D., & Vendelin, C.
- Venue
- Surgical Laparoscopy, Endoscopy & Percutaneous Techniques
- Published
- 2007-08
- Volume
- 17
- Issue
- 4
- Pages
- 291-295
- ISSN
- 1530-4515 (ISSN-L)
- OpenAlex
- W1981817457
- Cited by
- 27
- References
- 32
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2020: 2 · 2017: 1 · 2016: 3 · 2015: 1 · 2014: 1 · 2012: 2
- FWCI
- 1.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Surgical Laparoscopy Endoscopy & Percutaneous Techniques (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Abdominal vascular conditions and treatments
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal Bleeding Diagnosis and Treatment
- Health Sciences › Medicine › Surgery › Intestinal and Peritoneal Adhesions
- Keywords
- diagnostic laparoscopy, fluorescein, ultraviolet radiation, second-look laparoscopy, bowel resection, porcine model
- MeSH terms
- Animals, Disease Models, Animal, Embolization, Therapeutic, Female, Fluorescent Dyes, Intestines, Ischemia, Laparoscopy, Radiography, Swine, Ultraviolet Rays, Mesenteric Artery, Superior, Fluorescein
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review