P2X7 receptors enhance glutamate release in hippocampal hilar neurons
Abstract
We examined the effect of 2'-3'-O-(4-benzoylbenzoyl)-adenosine-5'-triphosphate (Bz-ATP), a P2X7 receptor agonist, on action potential-independent glutamate release from nerve terminals attached to mechanically isolated immature hilar neurons. Bz-ATP increased spontaneous excitatory postsynaptic current (sEPSC) frequency, and this effect was blocked by Brilliant blue G, a P2X7 receptor antagonist, suggesting that P2X7 receptors mediate the facilitatory action of Bz-ATP on sEPSCs. In most of hilar neurons tested, the Bz-ATP-induced increase in sEPSC frequency was blocked by tetrodotoxin or Cd, suggesting that the activation of P2X7 receptors leads to a presynaptic depolarization. The P2X7 receptor-mediated facilitation of glutamate release would modulate the excitability of hilar neurons, and eventually have a broad impact on the pathophysiological functions mediated by the hippocampus.
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Studies in pure neuronal cell cultures and synaptosomes confirm that stimulation of P2X7R expressed in nerve terminals favors glutamate and GABA release.
“The effects mediated by P2X7R in glial cells and adenosine can be avoided in studies in pure neuronal cell cultures [106] and synaptosomes [107,108,109], which confirms that stimulation of P2X7R expressed in nerve terminals favors glutamate and GABA release.”
From P2X7 Receptor Signaling in Stress and Depression (2019) · cites this paper as [107]
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- Authors
- Cho, J.-H., Choi, I.-S., & Jang, I.-S.
- Affiliations
- Kyungpook National University (KR)
- Corresponding author
- Jang, Il-Sung
- Venue
- Neuroreport
- Published
- 2010-09-15
- Volume
- 21
- Issue
- 13
- Pages
- 865-870
- ISSN
- 0959-4965 (ISSN-L)
- OpenAlex
- W2022693059
- Cited by
- 37
- References
- 22
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 4 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 1 · 2017: 5 · 2016: 2 · 2015: 7 · 2014: 3 · 2013: 2 · 2012: 1
- FWCI
- 1.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroreport (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Keywords
- P2X7 receptor, glutamate release, spontaneous excitatory postsynaptic currents, presynaptic facilitation, presynaptic depolarization, Brilliant Blue G, tetrodotoxin, cadmium
- MeSH terms
- Adenosine Triphosphate, Animals, Electrophysiology, Hippocampus, Synaptic Transmission, Neurons, Receptors, GABA-A, Tetrodotoxin, Receptors, N-Methyl-D-Aspartate, Rats, Sprague-Dawley, Presynaptic Terminals, Patch-Clamp Techniques, Glutamic Acid, Excitatory Postsynaptic Potentials, Rats, Receptors, Purinergic P2X7, Purinergic P2X Receptor Agonists, In Vitro Techniques
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