Potentiation of the glutamatergic synaptic input to rat locus coeruleus neurons by P2X7 receptors
Open accessAbstract
Locus coeruleus (LC) neurons in a rat brain slice preparation were superfused with a Mg(2+)-free and bicuculline-containing external medium. Under these conditions, glutamatergic spontaneous excitatory postsynaptic currents (sEPSCs) were recorded by means of the whole-cell patch-clamp method. ATP, as well as its structural analogue 2-methylthio ATP (2-MeSATP), both caused transient inward currents, which were outlasted by an increase in the frequency but not the amplitude of the sEPSCs. PPADS, but not suramin or reactive blue 2 counteracted both effects of 2-MeSATP. By contrast, α,β-methylene ATP (α,β-meATP), UTP and BzATP did not cause an inward current response. Of these latter agonists, only BzATP slightly facilitated the sEPSC amplitude and strongly potentiated its frequency. PPADS and Brilliant Blue G, as well as fluorocitric acid and aminoadipic acid prevented the activity of BzATP. Furthermore, BzATP caused a similar facilitation of the miniature (m)EPSC (recorded in the presence of tetrodotoxin) and sEPSC frequencies (recorded in its absence). Eventually, capsaicin augmented the frequency of the sEPSCs in a capsazepine-, but not PPADS-antagonizable, manner. In conclusion, the stimulation of astrocytic P2X7 receptors appears to lead to the outflow of a signalling molecule, which presynaptically increases the spontaneous release of glutamate onto LC neurons from their afferent fibre tracts. It is suggested, that the two algogenic compounds ATP and capsaicin utilise separate receptor systems to potentiate the release of glutamate and in consequence to increase the excitability of LC neurons.
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Participation of P2X7R localized in glial cells on the net effect on glutamate release has been indicated by radiolabeled studies in murine cortical astrocyte cultures and whole-cell patch-clamp analysis.
“However, participation of P2X7R localized in glial-cells on the net effect on glutamate release has also been indicated by radiolabeled studies in murine cortical astrocyte cultures [110] and whole-cell patch-clamp analysis [111].”
From P2X7 Receptor Signaling in Stress and Depression (2019) · cites this paper as [111]
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Cites
International Union of Pharmacology: Approaches to the Nomenclature of Voltage-Gated Ion Channels
Catterall, W. A., Chandy, K. G., Clapham, D. E., Gutman, G. A., Hofmann, F., Harmar, A. J., Abernethy, D. R., & Spedding, M. (2003) · Pharmacological Reviews · doi:10.1124/pr.55.4.5 · Cited by 7,126 · see citing works
The P2X7 Receptor: A Key Player in IL-1 Processing and Release
Ferrari, D., Pizzirani, C., Adinolfi, E., Lemoli, R. M., Curti, A., Idzko, M., Panther, E., & Di Virgilio, F. (2006) · The Journal of Immunology · doi:10.4049/jimmunol.176.7.3877 · Cited by 1,025 · see citing works
Purinergic signalling in neuron–glia interactions
Fields, R. D., & Burnstock, G. (2006) · Nature reviews. Neuroscience · doi:10.1038/nrn1928 · Cited by 852 · see citing works
Khakh, B. S., Burnstock, G., Kennedy, C., King, B. F., North, R. A., Séguéla, P., Voigt, M. M., & Humphrey, P. P. A. (2001) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01482-0 · Cited by 667 · see citing works
Barry, P. H. (1994) · Journal of Neuroscience Methods · doi:10.1016/0165-0270(94)90031-0 · Cited by 611 · see citing works
TRP Channel Proteins and Signal Transduction
Minke, B., & Cook, B. (2002) · Physiological Reviews · doi:10.1152/physrev.00001.2002 · Cited by 584 · see citing works
Qu, Y., Franchi, L., Nunez, G., & Dubyak, G. R. (2007) · The Journal of Immunology · doi:10.4049/jimmunol.179.3.1913 · Cited by 563 · see citing works
Signaling at Purinergic P2X Receptors
Surprenant, A., & North, R. A. (2008) · Annual Review of Physiology · doi:10.1146/annurev.physiol.70.113006.100630 · Cited by 547 · see citing works
Pharmacological profiles of cloned mammalian P2Y-receptor subtypes
von Kügelgen, I. (2005) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2005.08.014 · Cited by 541 · see citing works
Historical review: ATP as a neurotransmitter
Burnstock, G. (2006) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2006.01.005 · Cited by 539 · see citing works
P2X 7 Receptors Mediate ATP Release and Amplification of Astrocytic Intercellular Ca 2+ Signaling
Suadicani, S. O., Brosnan, C. F., & Scemes, E. (2006) · Journal of Neuroscience · doi:10.1523/jneurosci.3902-05.2006 · Cited by 512 · see citing works
Liaisons dangereuses: P2X7 and the inflammasome
Di Virgilio, F. (2007) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2007.07.002 · Cited by 493 · see citing works
P2X <sub>7</sub> Receptor-Mediated Release of Excitatory Amino Acids from Astrocytes
Duan, S., Anderson, C. M., Keung, E. C., Chen, Y., Chen, Y., & Swanson, R. A. (2003) · The Journal of Neuroscience · doi:10.1523/jneurosci.23-04-01320.2003 · Cited by 489 · see citing works
Pore dilation of neuronal P2X receptor channels
Virginio, C., MacKenzie, A., Rassendren, F. A., North, R. A., & Surprenant, A. (1999) · Nature Neuroscience · doi:10.1038/7225 · Cited by 414 · see citing works
P2X7 receptors in the nervous system
Sperlágh, B., Vizi, E. S., Wirkner, K., & Illés, P. (2006) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2006.03.007 · Cited by 374 · see citing works
Self-citationJarvis, M. F., & Khakh, B. S. (2008) · Neuropharmacology · doi:10.1016/j.neuropharm.2008.06.067 · Cited by 327 · see citing works
Chizh, B. A., & Illés, P. (2001) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01512-6 · Cited by 271 · see citing works
Stamford, J. (1995) · British Journal of Anaesthesia · doi:10.1093/bja/75.2.217 · Cited by 229 · see citing works
Emerging challenges of assigning P2X7 receptor function and immunoreactivity in neurons
Anderson, C. M., & Nedergaard, M. (2006) · Trends in Neurosciences · doi:10.1016/j.tins.2006.03.003 · Cited by 207 · see citing works
Aghajanian, G. K., & Wang, Y.-Y. (1987) · Neuropharmacology · doi:10.1016/0028-3908(87)90054-2 · Cited by 205 · see citing works
Noradrenaline‐mediated synaptic inhibition in rat locus coeruleus neurones.
Egan, T. M., Henderson, G., North, R. A., & Williams, J. T. (1983) · The Journal of Physiology · doi:10.1113/jphysiol.1983.sp014990 · Cited by 199 · see citing works
P2X 7 receptors control 2-arachidonoylglycerol production by microglial cells
Witting, A., Walter, L. M., Wacker, J. L., Möller, T., & Stella, N. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0306707101 · Cited by 187 · see citing works
Nakatsuka, T., & Gu, J. G. (2001) · Journal of Neuroscience · doi:10.1523/jneurosci.21-17-06522.2001 · Cited by 187 · see citing works
P2X1and P2X5Subunits Form the Functional P2X Receptor in Mouse Cortical Astrocytes
Lalo, U., Pankratov, Y. V., Wichert, S. P., Rossner, M. J., North, R. A., Kirchhoff, F., & Verkhratsky, A. (2008) · Journal of Neuroscience · doi:10.1523/jneurosci.1149-08.2008 · Cited by 171 · see citing works
Purinergic Receptors Mediate Two Distinct Glutamate Release Pathways in Hippocampal Astrocytes
Fellin, T., Pozzan, T., & Carmignoto, G. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m510679200 · Cited by 165 · see citing works
Hugel, S., & Schlichter, R. (2000) · Journal of Neuroscience · doi:10.1523/jneurosci.20-06-02121.2000 · Cited by 164 · see citing works
Capsaicin activation of glutamatergic synaptic transmission in the rat locus coeruleus In vitro
Marinelli, S., Vaughan, C. W., Christie, M. J., & Connor, M. A. (2002) · The Journal of Physiology · doi:10.1113/jphysiol.2002.022863 · Cited by 155 · see citing works
Molecular physiology of P2 receptors in the central nervous system
Illés, P., & Ribeiro, J. A. (2003) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2003.10.030 · Cited by 155 · see citing works
Fluoroacetate and fluorocitrate: Mechanism of action
Clarke, D. D. (1991) · Neurochemical Research · doi:10.1007/bf00965850 · Cited by 145 · see citing works
Role of ATP in fast excitatory synaptic potentials in locus coeruleus neurones of the rat
Nieber, K., Poelchen, W., & Illés, P. (1997) · British Journal of Pharmacology · doi:10.1038/sj.bjp.0701386 · Cited by 144 · see citing works
ATP Receptor-Mediated Enhancement of Fast Excitatory Neurotransmitter Release in the Brain
Khakh, B. S., & Henderson, G. (1998) · Molecular Pharmacology · doi:10.1124/mol.54.2.372 · Cited by 143 · see citing works
Properties and glial origin of osmotic‐dependent release of taurine from the rat supraoptic nucleus
Deleuze, C., Duvoid, A., & Hussy, N. (1998) · The Journal of Physiology · doi:10.1111/j.1469-7793.1998.463bt.x · Cited by 131 · see citing works
Roles of P2 receptors in glial cells: focus on astrocytes
Abbracchio, M. P., & Ceruti, S. (2006) · Purinergic Signalling · doi:10.1007/s11302-006-9016-0 · Cited by 116 · see citing works
Shigetomi, E., & Kato, F. (2004) · Journal of Neuroscience · doi:10.1523/jneurosci.0090-04.2004 · Cited by 113 · see citing works
Shen, K.-Z., & North, R. (1993) · Journal of Neuroscience · doi:10.1523/jneurosci.13-03-00894.1993 · Cited by 100 · see citing works
Co-transmitter function of ATP in central catecholaminergic neurons of the rat
Poelchen, W., Sieler, D., Wirkner, K., & Illés, P. (2001) · Neuroscience · doi:10.1016/s0306-4522(00)00529-7 · Cited by 94 · see citing works
Self-citationRhee, J. S., Wang, Z. M., Nabekura, J., et al. (2000) · The Journal of Physiology · doi:10.1111/j.1469-7793.2000.t01-1-00471.x · Cited by 94 · see citing works
Depolarization of rat locus coeruleus neurons by adenosine 5′-triphosphate
Harms, L., Finta, E. P., Tschöpl, M., & Illés, P. (1992) · Neuroscience · doi:10.1016/0306-4522(92)90282-7 · Cited by 91 · see citing works
Wirkner, K., Köfalvi, A., Fischer, W., Günther, A., Franke, H., Gröger‐Arndt, H., Nörenberg, W., Madarász, E., Vizi, E. S., Schneider, D., et al. (2005) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2005.03465.x · Cited by 89 · see citing works
Self-citationHuck, S., Grass, F., & Hörtnagl, H. (1984) · Journal of Neuroscience · doi:10.1523/jneurosci.04-10-02650.1984 · Cited by 84 · see citing works
Integration in the Ventral Medulla and Coordination of Sympathetic, Pain and Arousal Functions
Bockstaele, E. J. V., & Aston‐Jones, G. (1995) · Clinical and Experimental Hypertension · doi:10.3109/10641969509087062 · Cited by 83 · see citing works
Excitatory effects of adenosine 5′-triphosphate on rat locus coeruleus neurones
Tschöpl, M., Harms, L., Nörenberg, W., & Illés, P. (1992) · European Journal of Pharmacology · doi:10.1016/0014-2999(92)90234-u · Cited by 79 · see citing works
The role of P2X7 in pain and inflammation
Hughes, J. P., Hatcher, J. P., & Chessell, I. P. (2007) · Purinergic Signalling · doi:10.1007/s11302-006-9031-1 · Cited by 77 · see citing works
Carrasquero, L. M. G., Delicado, E. G., Bustillo, D., Gutiérrez-Martı́n, Y., Artalejo, A. R., & Miras‐Portugal, M. T. (2009) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2009.06179.x · Cited by 76 · see citing works
Synaptic potentials in rat locus coeruleus neurones.
Cherubini, E., North, R. A., & Williams, J. T. (1988) · The Journal of Physiology · doi:10.1113/jphysiol.1988.sp017389 · Cited by 74 · see citing works
Molecular Pain, a New Era of Pain Research and Medicine
Gu, J. G., Zhuo, M., Caterina, M. J., et al. (2005) · Molecular Pain · doi:10.1186/1744-8069-1-1 · Cited by 67 · see citing works
Synaptic potentials in locus coeruleus neurons in brain slices
Williams, J. T., Bobker, D. H., & Harris, G. C. M. (1991) · Progress in brain research · doi:10.1016/s0079-6123(08)63806-6 · Cited by 64 · see citing works
Cell-specific Behavior of P2X7 Receptors in Mouse Parotid Acinar and Duct Cells
Li, Q., Luo, X., Zeng, W., & Muallem, S. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m308306200 · Cited by 63 · see citing works
McBean, G. J. (1994) · British Journal of Pharmacology · doi:10.1111/j.1476-5381.1994.tb17022.x · Cited by 62 · see citing works
Pharmacological characterization of P2 purinoceptor types in rat locus coeruleus neurons
Fröhlich, R., Boehm, S., & Illés, P. (1996) · European Journal of Pharmacology · doi:10.1016/s0014-2999(96)00612-7 · Cited by 53 · see citing works
Watano, T., Calvert, J. A., Vial, C., et al. (2004) · The Journal of Physiology · doi:10.1113/jphysiol.2004.066845 · Cited by 46 · see citing works
A P2X Receptor-Mediated Nociceptive Afferent Pathway to Lamina I of the Spinal Cord
Chen, M., & Gu, J. G. (2005) · Molecular Pain · doi:10.1186/1744-8069-1-4 · Cited by 38 · see citing works
Negulyaev, Y. A., & Markwárdt, F. (2000) · Neuroscience Letters · doi:10.1016/s0304-3940(99)00976-3 · Cited by 30 · see citing works
Jameson, H. S., Piñol, R. A., & Mendelowitz, D. (2008) · Brain Research · doi:10.1016/j.brainres.2008.06.012 · Cited by 20 · see citing works
Wirkner, K., Franke, H., Inoue, K., et al. (1998) · Naunyn-Schmiedeberg s Archives of Pharmacology · doi:10.1007/pl00005154 · Cited by 16 · see citing works
Self-citation繁冨, 英. (2005) · Medical Entomology and Zoology · Cited by 12 · see citing works
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Sperlágh, B., & Illes, P. (2014) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2014.08.002 · Cited by 390 · see citing works
Neuronal P2X7 Receptors Revisited: Do They Really Exist?
Illes, P., Khan, T. M., & Rubini, P. (2017) · The Journal of Neuroscience · doi:10.1523/jneurosci.3103-16.2017 · Cited by 179 · see citing works
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- Authors
- Khakpay, R., Polster, D., Köles, L., Skorinkin, A., Szabo, B., Wirkner, K., & Illes, P.
- Affiliations
- Leipzig University (DE)
- Kazan Federal University (RU)
- ORCID
- Wirkner, Kerstin
- Venue
- Purinergic Signalling
- Published
- 2010-07-30
- Volume
- 6
- Issue
- 3
- Pages
- 349-359
- ISSN
- 1573-9538 (ISSN-L)
- 1573-9546
- PMC
- PMC2947656
- OpenAlex
- W1976813835
- Cited by
- 24
- References
- 56
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2021: 2 · 2019: 1 · 2017: 2 · 2016: 1 · 2015: 3 · 2014: 3 · 2013: 2 · 2012: 4
- FWCI
- 1.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Purinergic Signalling (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Deutscher Akademischer Austauschdienst
- Deutsche Forschungsgemeinschaft (IL 20/16-1)
- Volkswagen Foundation
- Tarbiat Modares University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
- Keywords
- locus coeruleus, P2X7 receptor, glutamatergic synaptic input, spontaneous excitatory postsynaptic currents, ATP, 2-methylthio-ATP, BzATP, presynaptic glutamate release, astrocytes, capsaicin, PPADS, neuronal excitability
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