Detecting adenosine triphosphate in the pericellular space
Open accessAbstract
Release of adenosine triphosphate (ATP) into the extracellular space occurs in response to a multiplicity of physiological and pathological stimuli in virtually all cells and tissues. A role for extracellular ATP has been identified in processes as different as neurotransmission, endocrine and exocrine secretion, smooth muscle contraction, bone metabolism, cell proliferation, immunity and inflammation. However, ATP measurement in the extracellular space has proved a daunting task until recently. To tackle this challenge, some years ago, we designed and engineered a novel luciferase probe targeted to and expressed on the outer aspect of the plasma membrane. This novel probe was constructed by appending to firefly luciferase the N-terminal leader sequence and the C-terminal glycophosphatidylinositol anchor of the folate receptor. This chimeric protein, named plasma membrane luciferase, is targeted and localized to the outer side of the plasma membrane. With this probe, we have generated stably transfected HEK293 cell clones that act as an in vitro and in vivo sensor of the extracellular ATP concentration in several disease conditions, such as experimentally induced tumours and inflammation.
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ATP concentration is present in the nanomolar range in the extracellular space of healthy tissues.
“ATP concentration is present in the nanomolar range in extracellular space of healthy tissues [67].”
From Hyperactivation of P2X7 receptors as a culprit of COVID-19 neuropathology (2021) · cites this paper as [67]
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The P2X7 Receptor: Central Hub of Brain Diseases
Andrejew, R., Oliveira-Giacomelli, Á., Ribeiro, D. E., Glaser, T., Arnaud-Sampaio, V. F., Lameu, C., & Ulrich, H. (2020) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2020.00124 · Cited by 162 · see citing works
Hyperactivation of P2X7 receptors as a culprit of COVID-19 neuropathology
Ribeiro, D. E., Oliveira-Giacomelli, Á., Glaser, T., Arnaud-Sampaio, V. F., Andrejew, R., Dieckmann, L., Baranova, J., Lameu, C., Ratajczak, M. Z., & Ulrich, H. (2021) · Molecular Psychiatry · doi:10.1038/s41380-020-00965-3 · Cited by 145 · see citing works
- Authors
- Falzoni, S., Donvito, G., & Di Virgilio, F.
- Affiliations
- University of Ferrara (IT)
- University of Ferrara , ,
- Venue
- Interface Focus
- Publisher
- The Royal Society
- Published
- 2013-06-06
- Volume
- 3
- Issue
- 3
- Pages
- 20120101-20120101
- ISSN
- 2042-8898 (ISSN-L)
- 2042-8901
- PMC
- PMC3638417
- OpenAlex
- W2112810110
- Cited by
- 136
- References
- 61
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 7 · 2023: 5 · 2022: 20 · 2021: 20 · 2020: 14 · 2019: 12 · 2018: 14 · 2017: 10 · 2016: 9 · 2015: 3 · 2014: 10
- FWCI
- 6.01
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Interface Focus (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Institutional Research Information System University of Ferrara (University of Ferrara) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Adenosine and Purinergic Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
- Keywords
- extracellular ATP, adenosine triphosphate detection, folate receptor, glycosylphosphatidylinositol anchor, firefly luciferase, tumours, inflammation, HEK293 cells
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