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Cited by 75Forensic and Genetic ResearchGenetic diversity and population structurePaleopathology and ancient diseases

Analysis of ancient DNA from a prehistoric Amerindian cemetery

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Stone, Anne C., Stoneking, Mark·1999·Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences
DOI 10.1098/rstb.1999.0368PMC PMC1692451
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Abstract

The Norris Farms No. 36 cemetery in central Illinois has been the subject of considerable archaeological and genetic research. Both mitochondrial DNA (mtDNA) and nuclear DNA have been examined in this 700–year–old population. DNA preservation at the site was good, with about 70% of the samples producing mtDNA results and approximately 15% yielding nuclear DNA data. All four of the major Amerindian mtDNA haplogroups were found, in addition to a fifth haplogroup. Sequences of the first hypervariable region of the mtDNA control region revealed a high level of diversity in the Norris Farms population and confirmed that the fifth haplogroup associates with Mongolian sequences and hence is probably authentic. Other than a possible reduction in the number of rare mtDNA lineages in many populations, it does not appear as if European contact significantly altered patterns of Amerindian mtDNA variation, despite the large decrease in population size that occurred. For nuclear DNA analysis, a novel method for DNA–based sex identification that uses nucleotide differences between the X and Y copies of the amelogenin gene was developed and applied successfully in approximately 20 individuals. Despite the well–known problems of poor DNA preservation and the ever–present possibility of contamination with modern DNA, genetic analysis of the Norris Farms No. 36 population demonstrates that ancient DNA can be a fruitful source of new insights into prehistoric populations.

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  • The Norris Farms #36 cemetery was selected for analysis because bone samples from this site had been previously investigated using ancient DNA techniques, and mtDNA haplogroup assignments and HVRI sequences are available for 108 and 52 individuals, respectively, making it the most intensively studied pre-Contact North American site to date.

    “The Norris Farms #36 cemetery was selected for analysis because bone samples from this site had been previously investigated using ancient DNA techniques (Stone and Stoneking, 1993, 1998, 1999), and mtDNA haplogroup assignments and HVRI sequences are available for 108 and 52 individuals, respectively, making it the most intensively studied pre‐Contact North American site to date.”

    From Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus (2016) · cites this paper as Stone and Stoneking, 1999

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Cited by

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Authors
Stone, A. C., & Stoneking, M.
Affiliations
Pennsylvania State University (US)
ORCID
Venue
Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences
Publisher
The Royal Society
Published
1999-01-29
Volume
354
Issue
1379
Pages
153-159
ISSN
  • 0962-8436 (ISSN-L)
  • 1471-2970
DOI
10.1098/rstb.1999.0368
PMC
PMC1692451
OpenAlex
W2015876096
Cited by
75
References
1
Citations by year
2026: 1 · 2022: 2 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 3 · 2014: 4 · 2013: 3
FWCI
1.24
Type
journal article
Language
en
Open access
Licence
royalsociety.org/journals/ethics-policies/data-sharing-mining/ (tdm)
Locations
  • Philosophical Transactions of the Royal Society B Biological Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • MPG.PuRe (Max Planck Society) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Forensic and Genetic Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
  • Social Sciences › Arts and Humanities › Archeology › Paleopathology and ancient diseases
Keywords
ancient DNA, mtDNA haplogroups, nuclear DNA, European contact, population size reduction, DNA contamination, prehistoric population genetics
MeSH terms
Dental Enamel Proteins, DNA, DNA, Mitochondrial, Female, Genetics, Population, Haplotypes, Humans, Illinois, Indians, North American, Male, Paleontology, Sex Determination Analysis, Genetic Variation, Polymerase Chain Reaction, Evolution, Molecular, History, Medieval, Amelogenin

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