The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Critical factors for assembling a high volume of DNA barcodes
Open accessAbstract
Large-scale DNA barcoding projects are now moving toward activation while the creation of a comprehensive barcode library for eukaryotes will ultimately require the acquisition of some 100 million barcodes. To satisfy this need, analytical facilities must adopt protocols that can support the rapid, cost-effective assembly of barcodes. In this paper we discuss the prospects for establishing high volume DNA barcoding facilities by evaluating key steps in the analytical chain from specimens to barcodes. Alliances with members of the taxonomic community represent the most effective strategy for provisioning the analytical chain with specimens. The optimal protocols for DNA extraction and subsequent PCR amplification of the barcode region depend strongly on their condition, but production targets of 100K barcode records per year are now feasible for facilities working with compliant specimens. The analysis of museum collections is currently challenging, but PCR cocktails that combine polymerases with repair enzyme(s) promise future success. Barcode analysis is already a cost-effective option for species identification in some situations and this will increasingly be the case as reference libraries are assembled and analytical protocols are simplified.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Instability and decay of the primary structure of DNA
Lindahl, T. (1993) · Nature · doi:10.1038/362709a0 · Cited by 5,679
Nondestructive DNA extraction method for mitochondrial DNA analyses of museum specimens
Rohland, N., Siedel, H., & Hofreiter, M. (2004) · BioTechniques · doi:10.2144/04365st05 · Cited by 183
Cited by
bold : The Barcode of Life Data System (http://www.barcodinglife.org)
RATNASINGHAM, S., & HEBERT, P. D. N. (2007) · Molecular Ecology Notes · doi:10.1111/j.1471-8286.2007.01678.x · Cited by 6,376
Self-citationDNA barcoding: how it complements taxonomy, molecular phylogenetics and population genetics
Hajibabaei, M., Singer, G. A. C., Hebert, P. D. N., & Hickey, D. A. (2007) · Trends in Genetics · doi:10.1016/j.tig.2007.02.001 · Cited by 1,160
Self-citationValentini, A., Pompanon, F., & Taberlet, P. (2009) · Trends in Ecology & Evolution · doi:10.1016/j.tree.2008.09.011 · Cited by 1,122
The campaign to DNA barcode all fishes, FISH‐BOL
Ward, R. D., Hanner, R., & Hebert, P. D. N. (2009) · Journal of Fish Biology · doi:10.1111/j.1095-8649.2008.02080.x · Cited by 964
Self-citationDNA barcoding the floras of biodiversity hotspots
Lahaye, R., van der Bank, M., Bogarin, D., Warner, J., Pupulin, F., Gigot, G., Maurin, O., Duthoit, S., Barraclough, T. G., & Savolainen, V. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0709936105 · Cited by 911
Metadata
- Authors
- Hajibabaei, M., deWaard, J. R., Ivanova, N. V., Ratnasingham, S., Dooh, R. T., Kirk, S. L., Mackie, P. M., & Hebert, P. D. N.
- Venue
- Philosophical Transactions of the Royal Society B: Biological Sciences
- Publisher
- The Royal Society
- Published
- 2005-09-08
- Volume
- 360
- Issue
- 1462
- Pages
- 1959-1967
- ISSN
- 0962-8436 (ISSN-L)
- 1471-2970
- OpenAlex
- W2148271075
- Cited by
- 539
- References
- 3
- FWCI
- 20.20
- Type
- journal article
- Language
- en
- Open access
- green
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