Rhodovulum iodosum sp. nov. and Rhodovulum robiginosum sp. nov., two new marine phototrophic ferrous-iron-oxidizing purple bacteria
Abstract
Two new strains of marine purple bacteria, N1T and N2T, were isolated from coastal sediment of the North Sea (Germany) with ferrous iron as the only electron donor for anoxygenic photosynthesis. The isolates are the first salt-dependent, ferrous-iron-oxidizing purple bacteria characterized so far. Analysis of 16S rRNA gene sequences revealed an affiliation with the genus Rhodovulum, which until now comprises only marine species. The sequence similarity of both strains was 95.2%, and their closest relative was Rhodovulum adriaticum. Like all known Rhodovulum species, the new strains had ovoid to rod-shaped cells, contained bacteriochlorophyll a and carotenoids of the spheroidene series, and were able to oxidize sulfide and thiosulfate. Like Rhodovulum adriaticum, both strains were unable to assimilate sulfate; for growth they needed a reduced sulfur source, e.g. thiosulfate. In contrast to the new strains, none of the known Rhodovulum species tested was able to oxidize ferrous iron or iron sulfide. In growth experiments, strains N1T and N2T oxidized 65 and 95%, respectively, of the ferrous iron supplied. Electron diffraction analysis revealed ferrihydrite as the main product of ferrous iron oxidation. In addition, traces of magnetite were formed. Strains N1T (= DSM 12328T) and N2T (= DSM 12329T) are described as Rhodovulum iodosum sp. nov. and Rhodovulum robiginosum sp. nov., respectively.
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Several experimental studies have confirmed that various purple and green bacteria can use Fe(II) as a reductant for CO2 fixation.
“Since then, several experimental studies have confirmed that various purple and green bacteria can use Fe(II) as a reductant for CO2 fixation (e.g., Widdel et al., 1993; Heising et al., 1999; Straub et al., 1999).”
From Iron formations: A global record of Neoarchaean to Palaeoproterozoic environmental history (2017) · cites this paper as Straub et al., 1999
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- Authors
- Straub, K. L., Rainey, F. A., & Widdel, F.
- Affiliations
- Max Planck Society (DE)
- Louisiana State University (US)
- Corresponding author
- Straub, Kristina L.
- ORCID
- Straub, Kristina L.
- Venue
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
- Publisher
- Microbiology Society
- Published
- 1999-04-01
- Volume
- 49
- Issue
- 2
- Pages
- 729-735
- ISSN
- 1466-5026 (ISSN-L)
- 1466-5034
- OpenAlex
- W2107900727
- Cited by
- 140
- References
- 21
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 5 · 2023: 6 · 2022: 6 · 2021: 7 · 2020: 2 · 2019: 6 · 2018: 7 · 2017: 8 · 2016: 4 · 2015: 8 · 2014: 8 · 2013: 5 · 2012: 4
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (publishedVersion)
- PubMed (publishedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Max-Planck-Gesellschaft
- Topics
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Physical Sciences › Engineering › Biomedical Engineering › Metal Extraction and Bioleaching
- Physical Sciences › Environmental Science › Environmental Engineering › Microbial Fuel Cells and Bioremediation
- MeSH terms
- Bacteria, Base Composition, DNA, Bacterial, DNA, Ribosomal, Ferrous Compounds, Molecular Sequence Data, North Sea, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S, Water Microbiology, Bacterial Physiological Phenomena, Geologic Sediments, Genes, rRNA, Photosynthetic Reaction Center Complex Proteins, Light-Harvesting Protein Complexes
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