The Temperature Sensitivity of the Sabin Type 3 Vaccine Strain of Poliovirus: Molecular and Structural Effects of a Mutation in the Capsid Protein VP3
Abstract
The growth of the Sabin strain of type 3 poliovirus is reduced at high temperatures compared to that of its virulent precursor strain Leon. Recombinant viruses have been generated from infectious cDNA clones and demonstrate that the temperature-sensitive (ts) phenotype is mainly attributable to a difference in residue 91 of the virion protein VP3. Examination of non-ts mutants derived in vitro or in vivo reveals the existence of second site mutations some of which are clearly able to suppress the ts phenotype. The location of residue 91 of VP3, and of a number of candidate suppressor mutations, in the atomic structure of the virion suggests that the ts phenotype may result in destabilization of the particle and that the suppressors may function by stabilizing specific interfaces. It is not yet clear whether the ts phenotype is expressed at the level of the particle or in the form of defects in assembly or uncoating of the virion, or all three.
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Antigenic evolution normally occurs during poliovirus replication.
“Antigenic evolution normally occurs during poliovirus replication”
From VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION (2005) · cites this paper as [129]
Cites
Three-Dimensional Structure of Poliovirus at 2.9 Å Resolution
Hogle, J. M., Chow, M., & Filman, D. J. (1985) · Science · doi:10.1126/science.2994218 · Cited by 1,262 · see citing works
Self-citationCloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
Racaniello, V. R., & Baltimore, D. (1981) · Science · doi:10.1126/science.6272391 · Cited by 696 · see citing works
The Site of Attachment in Human Rhinovirus 14 for Antiviral Agents That Inhibit Uncoating
Smith, T. J., Kremer, M. J., Luo, M., et al. (1986) · Science · doi:10.1126/science.3018924 · Cited by 456 · see citing works
History of Sabin attenuated poliovirus oral live vaccine strains
Sabin, A. B., & Boulger, L. R. (1973) · Journal of Biological Standardization · doi:10.1016/0092-1157(73)90048-6 · Cited by 318 · see citing works
Complete nucleotide sequence of the attenuated poliovirus Sabin 1 strain genome.
Nomoto, A., Omata, T., Toyoda, H., Kuge, S., Horie, H., Kataoka, Y., Genba, Y., Nakano, Y., & Imura, N. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.19.5793 · Cited by 308 · see citing works
Antigenic Structure of Polioviruses of Serotypes 1, 2 and 3
Minor, P. D., Ferguson, M. A. J., Evans, D. M. A., Almond, J. W., & Icenogle, J. P. (1986) · Journal of General Virology · doi:10.1099/0022-1317-67-7-1283 · Cited by 306 · see citing works
Self-citationMinor, P. D., John, A., Ferguson, M. A. J., & Icenogle, J. P. (1986) · Journal of General Virology · doi:10.1099/0022-1317-67-4-693 · Cited by 203 · see citing works
Self-citationGenetic analysis of the attenuation phenotype of poliovirus type 1
Omata, T., Kohara, M., Kuge, S., Komatsu, T., Abe, S., Semler, B. L., Kameda, A., Itoh, H., Arita, M., & Wimmer, E. (1986) · Journal of Virology · doi:10.1128/jvi.58.2.348-358.1986 · Cited by 190 · see citing works
Killed poliovirus antigen titration in humans.
SALK, J. E., Cohen, H., Fillastre, C., Stoeckel, P., Rey, J., Schlumberger, M., Nicolas, A., van Steenis, G., van Wezel, A. L., Triau, R., et al. (1978) · PubMed · Cited by 26 · see citing works
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VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION
Kew, O. M., Sutter, R. W., de Gourville, E. M., Dowdle, W. R., & Pallansch, M. A. (2005) · Annual Review of Microbiology · doi:10.1146/annurev.micro.58.030603.123625 · Cited by 703 · see citing works
Macadam, A. J., Arnold, C., Howlett, J., John, A., Marsden, S., Taffs, F., Reeve, P., Hamada, N., Wareham, K., Almond, J., et al. (1989) · Virology · doi:10.1016/0042-6822(89)90183-9 · Cited by 125 · see citing works
Self-citationMacadam, A. J., Ferguson, G., Stone, D. M., Meredith, J., Knowlson, S., Auda, G., Almond, J. W., & Minor, P. D. (2006) · Journal of Virology · doi:10.1128/jvi.00370-06 · Cited by 89 · see citing works
Self-citationPoliovirus Vaccines: Molecular Biology and Immune Response
Minor, P. D., & Almond, J. (2014) · Molecular Biology of Picornavirus · doi:10.1128/9781555817916.ch30 · Cited by 33 · see citing works
Self-citationDeterminants of Poliovirus Pathogenesis
Gromeier, M., & Nomoto, A. (2014) · Molecular Biology of Picornavirus · doi:10.1128/9781555817916.ch29 · Cited by 20 · see citing works
- Authors
- Minor, P. D., Dunn, G., Evans, D. M. A., Magrath, D. I., John, A., Howlett, J., Phillips, A., Westrop, G., Wareham, K., Almond, J. W., & Hogle, J. M.
- Affiliations
- National Institute for Biological Standards and Control (GB)
- University of Reading (GB)
- Scripps Research Institute (US)
- Venue
- Journal of General Virology
- Publisher
- Microbiology Society
- Published
- 1989-05-01
- Volume
- 70
- Issue
- 5
- Pages
- 1117-1123
- ISSN
- 0022-1317 (ISSN-L)
- 1465-2099
- OpenAlex
- W2121253082
- Cited by
- 82
- References
- 9
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2020: 1 · 2017: 1 · 2016: 3 · 2015: 2 · 2014: 6 · 2012: 2
- FWCI
- 8.50
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of General Virology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Keywords
- temperature-sensitive phenotype, VP3 capsid protein, suppressor mutations, second-site mutations, virion stability, viral assembly, viral uncoating, infectious clone
- MeSH terms
- Amino Acid Sequence, Capsid, Genes, Viral, Mutation, Phenotype, Viral Plaque Assay, Poliovirus Vaccine, Oral, Recombination, Genetic, RNA, Viral, Suppression, Genetic, Temperature, Poliovirus
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