Cited by 67Evolution and Genetic DynamicsViral Infections and Immunology ResearchVirology and Viral Diseases

Rapid molecular evolution of wild type 3 poliovirus during infection in individual hosts

Kinnunen, L., Huovilainen, A., Poyry, T., Hovi, T.·1990·Journal of General Virology

Abstract

A panel of nine neutralizing monoclonal antibodies was used to analyse the antigenic properties of 188 plaque-purified type 3 poliovirus strains from 17 faecal specimens, derived from eight people during a 2 month observation period. Most poliovirus specimens consisted of a mixture of antigenically distinct variants and the composition of the mixture was found to change between sequential specimens in many individuals, indicating antigenic evolution. Thirty-five strains representing different antigenic patterns were selected for partial sequencing of genomic RNA. Mutations leading to amino acid substitutions, as well as silent mutations, were seen at and close to the known antigenic sites. The frequency of silent mutations was used to estimate the evolutionary potential of the virus. The largest difference in silent changes between strains isolated from one person was 0.8%, which corresponds to a minimum of about 60 mutations per genome within a period of 3 weeks. The observed incidence of silent mutations between isolates from different persons was usually between 0.8 and 2%. These figures agree with the previously reported overall mutation rates of poliovirus, determined by other methods.

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Crossref · 10.1099/0022-1317-71-2-317

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25 of 25 references verified0 citation errors · 0 not checkable

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  • The bottlenecks driving the rapid evolution of polioviruses appear to occur primarily during replication in the human intestine.

    “Interestingly, the bottlenecks driving the rapid evolution of polioviruses appear to occur primarily during replication in the human intestine (95, 109).”

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Authors
Kinnunen, L., Huovilainen, A., Poyry, T., & Hovi, T.
Venue
Journal of General Virology
Published
1990-02-01
Volume
71
Issue
2
Pages
317-324
ISSN
  • 0022-1317 (ISSN-L)
  • 1465-2099
OpenAlex
W2158172094
Cited by
67
References
25
Citations by year
2023: 1 · 2021: 2 · 2020: 1 · 2014: 7 · 2013: 2 · 2012: 2
FWCI
5.56
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of General Virology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
Keywords
poliovirus type 3, antigenic evolution, molecular evolution, neutralizing monoclonal antibodies, synonymous mutations, amino acid substitutions, viral mutation rate, antigenic determinants, fecal isolates
MeSH terms
Amino Acid Sequence, Antibodies, Monoclonal, Antigenic Variation, Antigens, Viral, Base Sequence, Disease Outbreaks, Feces, Finland, Humans, Molecular Sequence Data, Mutation, Neutralization Tests, Poliomyelitis, RNA, Viral, Poliovirus

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