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Cited by 408Bacterial biofilms and quorum sensingPlant Pathogenic Bacteria StudiesBurn Injury Management and Outcomes

Contribution of Quorum Sensing to the Virulence of Pseudomonas aeruginosa in Burn Wound Infections

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Rumbaugh, Kendra P., Griswold, John A., Iglewski, Barbara H., Hamood, Abdul N.·1999·Infection and Immunity
DOI 10.1128/iai.67.11.5854-5862.1999PMC PMC96966
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Abstract

The Pseudomonas aeruginosa quorum-sensing systems, las and rhl , control the production of numerous virulence factors. In this study, we have used the burned-mouse model to examine the contribution of quorum-sensing systems to the pathogenesis of P. aeruginosa infections in burn wounds. Different quorum-sensing mutants of P. aeruginosa PAO1 that were defective in the lasR , lasI , or rhlI gene or both the lasI and rhlI genes were utilized. The following parameters of the P. aeruginosa infection were examined: (i) lethality to the burned mouse, (ii) dissemination of the P. aeruginosa strain within the body of the infected mouse (by determining the numbers of CFU of P. aeruginosa within the liver and spleen), and (iii) spread of the P. aeruginosa strain within the burned skin (by determining the numbers of CFU of P. aeruginosa at the inoculation site and at a site about 15 mm from the inoculation site [distant site]). In comparison with that of PAO1, the in vivo virulence of lasI , lasR , and rhlI mutants was significantly reduced. However, the most significant reduction in in vivo virulence was seen with the lasI rhlI mutant. The numbers of CFU that were recovered from the livers, spleens, and skin of mice infected with different mutants were significantly lower than those of PAO1. At 8 and 16 h post burn infection, comparable numbers of CFU of PAO1 and lasI and rhlI mutants were obtained from both the inoculation and distant sites of the burned skin of infected mice. In contrast, CFU of the lasR mutant and the lasI rhlI double mutant were recovered only from the inoculation site of infected mice at 8 and 16 h post burn infection. The ability of a plasmid carrying either the lasI or rhlI gene or the lasI and rhlI genes to complement the defect of the lasI rhlI double mutant was also examined. The presence of any of these plasmids within the lasI rhlI double mutant significantly enhanced its in vivo virulence, as well as its ability to spread within the burned skin. These results suggest that the quorum-sensing systems play an important role in the horizontal spread of P. aeruginosa within burned skin and in the dissemination of P. aeruginosa within the bodies of burned-and-infected mice and contributed to the overall virulence of P. aeruginosa in this animal model.

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Authors
Rumbaugh, K. P., Griswold, J. A., Iglewski, B. H., & Hamood, A. N.
Affiliations
  • <!--label omitted: 1-->Department of Microbiology and Immunology1 and
  • Texas Tech University (US)
  • University of Rochester Medicine (US)
ORCID
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1999-11
Volume
67
Issue
11
Pages
5854-5862
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
DOI
10.1128/iai.67.11.5854-5862.1999
PMC
PMC96966
OpenAlex
W2138350975
Cited by
408
References
49
Citations by year
2026: 7 · 2025: 5 · 2024: 8 · 2023: 15 · 2022: 15 · 2021: 10 · 2020: 17 · 2019: 12 · 2018: 15 · 2017: 12 · 2016: 16 · 2015: 19 · 2014: 26 · 2013: 21 · 2012: 14
FWCI
4.95
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Infection and Immunity (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of Allergy and Infectious Diseases (AI33713, R37AI033713, R01AI033713)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogenic Bacteria Studies
  • Health Sciences › Medicine › Epidemiology › Burn Injury Management and Outcomes
Keywords
Pseudomonas aeruginosa, quorum sensing, burn wound infection, virulence factors, lasI, LasR, rhlI, bacterial dissemination, complementation, Pseudomonas aeruginosa PAO1, bacterial virulence genes, virulence gene regulation, virulence plasmid
MeSH terms
Animals, Burns, Female, Mutation, Plasmids, Pseudomonas aeruginosa, Skin, Virulence, Wound Infection, Mice

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