Cited by 91Reproductive tract infections researchProbiotics and Fermented FoodsGut microbiota and health

Selection of Lactobacillus strains as potential probiotics for vaginitis treatment

Santos, Carolina M. A., Pires, Maria C. V., Leão, Thiago L., Hernández, Zulema P., Rodriguez, Marisleydys L., Martins, Ariane K. S., Miranda, Lilian S., Martins, Flaviano S. +1 more·2016·Microbiology

Abstract

Lactobacilli are the dominant bacteria of the vaginal tract of healthy women, and imbalance of the local microbiota can predispose women to acquire infections, such as bacterial vaginosis (BV) and vulvovaginal candidiasis (VVC). Although antimicrobial therapy is generally effective, there is still a high incidence of recurrence and increase of microbial resistance due to the repetitive use of antimicrobials. Thus, it has been suggested that administration of probiotics incorporating selected Lactobacillus strains may be an effective strategy for preventing vaginal infections. Accordingly, the in vitro probiotic potential of 23 lactobacilli isolated from the vaginal ecosystem of healthy women from Cuba was evaluated for use in BV and VVC treatments. Eight strains were selected based on their antagonist potential against Gardnerella vaginalis, Candida albicansor both. In vitro assays revealed that all these strains reduced the pathogen counts in co-incubation, showed excellent adhesive properties (biofilm formation and auto-aggregation), were able to co-aggregate with G. vaginalis and C. albicans, yielded high amounts of hydrogen peroxide and lactic acid and demonstrated high adhesion rates to epithelial HeLa cells. Interference tests within HeLa cells showed that all strains were able to reduce the adherence of pathogens by exclusion or displacement. Lactobacilli were able to inhibit HeLa cell apoptosis caused by pathogens when the cells were incubated with the probiotics prior to challenge. These results suggest that these strains have a promising probiotic potential and can be used for prevention or treatment of BV and VVC.

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Crossref · 10.1099/mic.0.000302

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  • Several previous studies have shown that probiotic Lactobacillus can inhibit the C. albicans adherence on the vaginal and intestinal mucosal surfaces.

    “Regarding anti-Candida adhesion, several previous studies have also shown that probiotic Lactobacillus can inhibit the C. albicans adherence on the vaginal and intestinal mucosal surfaces.11, 12, 13, 14”
  • Previous studies reported that postbiotics could reduce the adhesion of C. albicans on vaginal epithelial cells by Lactobacillus CFSs, and biosurfactants.

    “Previous studies reported that postbiotics could reduce the adhesion of C. albicans on vaginal epithelial cells by Lactobacillus CFSs, and biosurfactants.13,14,17,18”

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Authors
Santos, C. M. A., Pires, M. C. V., Leão, T. L., Hernández, Z. P., Rodriguez, M. L., Martins, A. K. S., Miranda, L. S., Martins, F. S., & Nicoli, J. R.
Affiliations
  • Universidade Federal de Minas Gerais (BR)
  • Centro Nacional de Sanidad Agropecuária, Dirección de Producciones Biofarmacéuticas, San José de las Lajas Mayabeque, Cuba
  • Centro Nacional de Sanidad Agropecuaria (CU)
Venue
Microbiology
Published
2016-07-01
Volume
162
Issue
7
Pages
1195-1207
ISSN
  • 1350-0872 (ISSN-L)
  • 1465-2080
OpenAlex
W2345583598
Cited by
91
References
45
Citations by year
2026: 8 · 2025: 7 · 2024: 9 · 2023: 7 · 2022: 12 · 2021: 15 · 2020: 8 · 2019: 12 · 2018: 5 · 2017: 5 · 2016: 2
FWCI
8.18
Type
journal article
Language
en
Open access
closed
Locations
  • Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords
Lactobacillus, probiotics, bacterial vaginosis, vaginal candidiasis, Gardnerella vaginalis, Candida albicans, vaginal microbiota, antagonistic activity, biofilm formation, self-aggregation, cell adhesion, hydrogen peroxide, lactic acid, HeLa cells, apoptosis, vaginal infection
MeSH terms
Anti-Infective Agents, Bacterial Adhesion, Candida albicans, Candidiasis, Vulvovaginal, Cuba, Female, Gardnerella vaginalis, HeLa Cells, Humans, Hydrogen Peroxide, Lactobacillus, RNA, Ribosomal, 16S, Vagina, Vaginosis, Bacterial, Apoptosis, Biofilms, Lactic Acid, Probiotics, Cell Line, Tumor

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