Vaginal lactobacilli as potential probiotics against Candida spp
Open accessAbstract
Urogenital infections affect millions of people every year worldwide. The treatment of these diseases usually requires the use of antimicrobial agents, and more recently, the use of probiotic lactic acid bacteria (LAB) cultures for the management of vaginal infections has been extensively studied. In this work, 11 vaginal lactobacilli isolates, previously obtained from healthy patients, were studied to screen microorganisms with probiotic properties against Candida spp. The LAB were tested for their ability of auto-aggregation, co-aggregation with C. albicans, C. glabrata, C. krusei, and C. tropicalis, adhesion to Caco-2 epithelial cells and production of lactic acid and hydrogen peroxide (H2O2). All lactobacilli isolates tested were able to auto-aggregate (ranging from 25.3% to 75.4% assessed at 4 hours of incubation) and to co-aggregate with the four Candida species into different degrees; among them L. crispatus showed the highest scores of co-aggregation. The highest amount of lactic acid was produced by L. salivarius (13.9 g/l), followed by L. johnsonii (6.5 g/l), L. acidophilus (5.5 g/l), and L. jensenii (5.4 g/l). All isolates produced H2O2, but the highest levels (3 - 10 mg/l) were observed for L. acidophilus, L. crispatus, L. gasseri, L. johnsonii, and L. vaginalis. Only L. agilis, L. jensenii, L. johnsonii and L. ruminus were able to adhere to epithelial Caco-2 cells. Among the isolates evaluated, L agilis, L. jensenii, L. johnsonii, and L. ruminus exhibited simultaneously several desirable properties as potential probiotic strains justifying future studies to evaluate their technological properties in different pharmaceutical preparations for human use.
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Autoaggregation is the gathering of the same microorganism strains, which can create a barrier to prevent pathogen adhesion.
“Autoaggregation is the gathering of the same microorganism strains, which can create a barrier to prevent pathogen adhesion.”
From Aggregation and adhesion ability of various probiotic strains and Candida species: An in vitro study (2024) · cites this paper as [7]
The Results and Discussion section reports that lactobacilli auto-aggregate and that auto-aggregation is a first step toward biofilms that help inhibit pathogen overgrowth. It does not say that auto-aggregation itself creates a barrier preventing pathogen adhesion; it links barrier formation to lactobacilli adhering to epithelial tissue, and blocking pathogens from tissue receptors to co-aggregation. Page numbers are unavailable in our copy. (LLM, conf=0.83)
Experimental — accuracy not yet measuredPrevious studies have reported that the coaggregation ability of probiotic Lactobacillus strains with Candida species varied depending on the strain.
“Previous studies have reported that the coaggregation ability of probiotic Lactobacillus strains with Candida species varied depending on the strain, however, most studies have only examined intestinal and vaginal probiotic Lactobacillus strains.7, 8, 9”
From Aggregation and adhesion ability of various probiotic strains and Candida species: An in vitro study (2024) · cites this paper as [7]
Coaggregation is the gathering of different microorganism strains, which can inhibit the dissemination of the pathogen to adhesion sites on host mucosal tissues.
“Coaggregation is the gathering of different microorganism strains, which can inhibit the dissemination of the pathogen to adhesion sites on host mucosal tissues.7”
From Aggregation and adhesion ability of various probiotic strains and Candida species: An in vitro study (2024) · cites this paper as [7]
Most studies have only examined intestinal and vaginal probiotic Lactobacillus strains, and there have been very few studies relating to oral probiotic strains.
“however, most studies have only examined intestinal and vaginal probiotic Lactobacillus strains.7, 8, 9 There have been very few studies relating to oral probiotic strains.10”
From Aggregation and adhesion ability of various probiotic strains and Candida species: An in vitro study (2024) · cites this paper as [7]
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- Authors
- Gil, N. F., Martinez, R. C. R., Gomes, B. C., Nomizo, A., & Martinis, E. C. P. D.
- Affiliations
- Universidade de São Paulo (BR)
- Corresponding author
- Martinis, Elaine C. P. De
- Venue
- Brazilian Journal of Microbiology
- Publisher
- FapUNIFESP (SciELO)
- Published
- 2010-03
- Volume
- 41
- Issue
- 1
- Pages
- 6-14
- ISSN
- 1517-8382 (ISSN-L)
- PMC
- PMC3768620
- OpenAlex
- W2145995658
- Cited by
- 57
- References
- 43
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 2 · 2023: 4 · 2022: 4 · 2021: 7 · 2020: 8 · 2019: 4 · 2018: 2 · 2017: 6 · 2016: 8 · 2015: 3 · 2014: 4 · 2013: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- cc-by-nc
- Locations
- Brazilian Journal of Microbiology (publishedVersion, cc-by-nc)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc)
- PubMed Central (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas) (publishedVersion, other-oa)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (publishedVersion)
- Americanae (AECID Library) (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Keywords
- vaginal lactobacilli, probiotics, Candida, Candida albicans, Candida glabrata, Candida krusei, Candida tropicalis, self-aggregation, co-aggregation, Caco-2 cell adhesion, lactic acid production, hydrogen peroxide production, Lactobacillus crispatus, Lactobacillus johnsonii, Lactobacillus vaginalis, vaginal infection, urogenital infections
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