Genomic and proteomic characterization of gordonia sp. NB4-1Y in relation to 6: 2 fluorotelomer sulfonate biodegradation
Abstract
Gordonia sp. strain NB4-1Y was isolated from vermicompost using bis-(3-pentafluorophenylpropyl)-sulfide as the sole added sulfur source and was found to have a broad capacity for metabolizing organosulfur compounds. NB4-1Y is closely related to G. desulfuricans and was found to metabolize 6 : 2 fluorotelomer sulfonate (6 : 2 FTS) to 5 : 3 fluorotelomer acid (5 : 3 acid) via 6 : 2 fluorotelomer acid (6 : 2 FTCA), 6 : 2 unsaturated fluorotelomer acid (6 : 2 FTUCA) and 5 : 3 unsaturated fluorotelomer acid (5 : 3 Uacid). Given that the molecular and biochemical basis for the microbial metabolism of poly- and per-fluorinated compounds has yet to be examined, we undertook to investigate 6 : 2 FTS metabolism in NB4-1Y. To this end, a whole-genome shotgun sequence was prepared and two-dimensional differential in-gel electrophoresis was used to compare proteomes of MgSO4- and 6 : 2 FTS-grown cells. Of the three putative alkanesulfonate monooxygenases, four nitrilotriacetate monooxygenases and one taurine dioxygenase located in the draft genome, two nitrilotriacetate monooxygenases were differentially expressed in the presence of 6 : 2 FTS. It is hypothesized that these two enzymes may be responsible for 6 : 2 FTS desulfonation. In addition, a differentially expressed putative double bond reductase may be involved in the reduction of 5 : 3 Uacid to 5 : 3 acid. Other proteins differentially expressed during 6 : 2 FTS metabolism included a sulfate ABC transporter ATP-binding protein and…
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Monooxygenases with co-factors other than copper (e.g., FMNH2, NADP) are known to increase the biotransformation of fluorotelomer compounds.
“monooxygenases with other co-factors (e.g., reduced flavin mononucleotide (FMNH2), nicotinamide adenine dinucleotide phosphate (NADP)) are also known to increase the biotransformation of fluorotelomer compounds (Lewis et al., 2016; Van Hamme et al., 2013).”
From Formation of perfluorocarboxylic acids from 6:2 fluorotelomer sulfonate (6:2 FTS) in landfill leachate: Role of microbial communities (2020) · cites this paper as Van Hamme et al., 2013
Desulfonation of PFSAs is suspected to be catalyzed by alkanesulfonate monooxygenases, ssuDEABC proteins, haloacid dehalogenases, cytochrome P450s and nitriloacetate monooxygenases.
“is suspected to be catalyzed by alkanesulfonate monooxygenases, ssuDEABC proteins, haloacid dehalogenases, cytochrome P450s and nitriloacetate monooxygenases (Table 4) (Méndez et al., 2022; Van Hamme et al., 2013; Yang et al., 2022)”
From A review of microbial degradation of per- and polyfluoroalkyl substances (PFAS): Biotransformation routes and enzymes (2023) · cites this paper as Van Hamme et al., 2013
Cites
Quantitative determination of fluorotelomer sulfonates in groundwater by LC MS/MS
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“…biodegrade to 4 : 2, 6 : 2 and 8 : 2 fluorotelomer sulfonates [F(CF2)nCH2CH2SO3H, n54, 6, 8] as indicated by increased levels in groundwater at military firefighting-training facilities (Schultz et al., 2004) and their detection in fish livers following environmental release (Oakes et al., 2010).”
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Fluorotelomer Alcohol BiodegradationDirect Evidence that Perfluorinated Carbon Chains Breakdown
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Berhanu, A., Mutanda, I., Taolin, J., Qaria, M. A., Yang, B., & Zhu, D. (2023) · Science of The Total Environment · doi:10.1016/j.scitotenv.2022.160010 · Cited by 294 · see citing works
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Self-citationFate and transport of per- and polyfluoroalkyl substances (PFASs) in the vadose zone
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Aerobic biotransformation of fluorotelomer compounds in landfill leachate-sediment
Hamid, H., Li, L. Y., & Grace, J. R. (2020) · Science of The Total Environment · doi:10.1016/j.scitotenv.2020.136547 · Cited by 111 · see citing works
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- Authors
- Van Hamme, J. D., Bottos, E. M., Bilbey, N. J., & Brewer, S. E.
- Affiliations
- Department of Biological Sciences, Thompson Rivers University, Kamloops, British Columbia, Canada V2C 0C8
- Thompson Rivers University (CA)
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2013-08-01
- Volume
- 159
- Issue
- Pt_8
- Pages
- 1618-1628
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2158239176
- Cited by
- 66
- References
- 4
- Citations by year
- 2026: 8 · 2025: 18 · 2024: 10 · 2023: 4 · 2022: 4 · 2021: 4 · 2020: 3 · 2019: 4 · 2018: 1 · 2017: 3 · 2016: 4 · 2015: 1 · 2014: 2
- FWCI
- 1.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Microbiology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Guelph
- Topics
- Physical Sciences › Environmental Science › Environmental Chemistry › Per- and polyfluoroalkyl substances research
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Physical Sciences › Environmental Science › Environmental Chemistry › Environmental Chemistry and Analysis
- Keywords
- 6:2 fluorotelomer sulfonate, alkyl hydroperoxide reductase, differentially expressed proteins
- MeSH terms
- Actinomycetales, DNA, Bacterial, Genes, Bacterial, Hydrocarbons, Fluorinated, Molecular Sequence Data, Electrophoresis, Gel, Two-Dimensional, Sequence Analysis, DNA, Proteome, Gene Expression Profiling, Metabolic Networks and Pathways
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