The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes
Abstract
The aim of this study was to evaluate the use of RNA polymerase α subunit (rpoA) and phenylalanyl-tRNA synthase (pheS) gene sequences as species identification tools for enterococci. Ninety-six representative strains comprising all currently recognized Enterococcus species were examined.rpoA gene sequences generated a robust classification into species groups similar to the one based on 16S rRNA gene sequence analysis. On the other hand, the pheS gene is a fast-evolving clock even better suited for species delineation than the rpoA gene, but not for recognition of species groups within Enterococcus as determined by both rpoA and 16S rRNA genes. All enterococcal species were clearly differentiated on the basis of their rpoA and pheS sequences. Evaluation of intraspecies variation showed that both rpoA and pheS genes have a high degree of homogeneity among strains of the same species. Strains of the same enterococcal species have at least 99 %rpoA and 97 %pheS gene sequence similarity, whereas, different enterococcal species have at maximum 97 %rpoA and 86 %pheS gene sequence similarity. It was concluded that both genes can be used as reliable tools for identification of clinical and environmental species of Enterococcus and are efficient screening methods for the detection of novel species. The sequence data obtained in this study were compared to the available atpA and 16S rRNA gene sequences. The MLSA approach to Enterococcus taxonomy provides portable, highly reproducible data with lower costs for rapid identification of all enterococcal species.
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Cites
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
(1987) · Molecular Biology and Evolution · doi:10.1093/oxfordjournals.molbev.a040454 · Cited by 59,715
The life and times of the Enterococcus
Murray, B. E. (1990) · Clinical Microbiology Reviews · doi:10.1128/cmr.3.1.46 · Cited by 1,908
SplitsTree: analyzing and visualizing evolutionary data.
Huson, D. H. (1998) · Bioinformatics · doi:10.1093/bioinformatics/14.1.68 · Cited by 1,642
Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology.
Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rosselló-Mora, R., Swings, J., Trüper, H. G., et al. (2002) · International Journal of Systematic and Evolutionary Microbiology · doi:10.1099/00207713-52-3-1043 · Cited by 1,540
Self-citationDutka-Malen, S., Evers, S., & Courvalin, P. (1995) · Journal of Clinical Microbiology · doi:10.1128/jcm.33.1.24-27.1995 · Cited by 1,403
Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology
Stackebrandt, E. (2002) · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · doi:10.1099/ijs.0.02360-0 · Cited by 916
Jett, B. D., Huycke, M. M., & Gilmore, M. S. (1994) · Clinical Microbiology Reviews · doi:10.1128/cmr.7.4.462 · Cited by 820
Enterococci at the crossroads of food safety?
Franz, C. M. A. P., Holzapfel, W. H., & Stiles, M. E. (1999) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(99)00007-0 · Cited by 735
Applicability of rep-PCR fingerprinting for identification of Lactobacillus species
Gevers, D. (2001) · FEMS Microbiology Letters · doi:10.1016/s0378-1097(01)00439-6 · Cited by 630
Self-citationEnterococci in foods—a conundrum for food safety
Franz, C. M. A. P., Stiles, M. E., Schleifer, K. H., & Holzapfel, W. H. (2003) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(03)00174-0 · Cited by 568
Cited by
Di Cagno, R., De Angelis, M., De Pasquale, I., Ndagijimana, M., Vernocchi, P., Ricciuti, P., Gagliardi, F., Laghi, L., Crecchio, C., Guerzoni, M. E., et al. (2011) · BMC Microbiology · doi:10.1186/1471-2180-11-219 · Cited by 317
Biodiversity and identification of sourdough lactic acid bacteria
De Vuyst, L., & Vancanneyt, M. (2007) · Food Microbiology · doi:10.1016/j.fm.2006.07.005 · Cited by 232
Self-citationHuys, G., Botteldoorn, N., Delvigne, F., De Vuyst, L., Heyndrickx, M., Pot, B., Dubois, J., & Daube, G. (2013) · Molecular Nutrition & Food Research · doi:10.1002/mnfr.201300065 · Cited by 132
Pérez Montoro, B., Benomar, N., Caballero Gómez, N., Ennahar, S., Horvatovich, P., Knapp, C. W., Alonso, E., Gálvez, A., & Abriouel, H. (2018) · Food Research International · doi:10.1016/j.foodres.2018.04.072 · Cited by 46
Bottari, B., Agrimonti, C., Gatti, M., Neviani, E., & Marmiroli, N. (2013) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2012.10.011 · Cited by 45
Metadata
- Authors
- Naser, S. M., Thompson, F. L., Hoste, B., Gevers, D., Dawyndt, P., Vancanneyt, M., & Swings, J.
- Venue
- Microbiology
- Publisher
- Microbiology Society
- Published
- 2005-07-01
- Volume
- 151
- Issue
- 7
- Pages
- 2141-2150
- ISSN
- 1350-0872 (ISSN-L)
- 1465-2080
- OpenAlex
- W2165339408
- Cited by
- 409
- References
- 44
- FWCI
- 10.36
- Type
- journal article
- Language
- en
- Open access
- closed
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- Naser, S. M., Thompson, F. L., Hoste, B., Gevers, D., Dawyndt, P., Vancanneyt, M., & Swings, J. (2005). Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology. https://doi.org/10.1099/mic.0.27840-0
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