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Reactivation of human herpesvirus 6 during ex vivo expansion of circulating CD34+ haematopoietic stem cells
Abstract
Human herpesvirus 6 (HHV-6) replication was evaluated during in vitro expansion of CD34-positive cells that were selected from 11 peripheral blood progenitor cell (PBPC) samples. In order to permit cellular differentiation towards the myeloid lineage, PBPCs were cultured for 14-21 days in a liquid, serum-free medium supplemented with interleukin 1 (IL1), IL3, IL6, granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor and stem-cell factor. Among the 10 cultures from HHV-6-seropositive patients, the late, alternatively spliced U100 viral mRNA was detected in five of them after PBPC culture for 14 or 21 days. Recovery of infectious virus from one of the expansions, associated with an increase of HHV-6 viral load and detection of the U100 spliced messenger, confirmed the occurrence of a complete replicative cycle. These data thus demonstrate for the first time that haematopoietic differentiation can lead to HHV-6 reactivation.
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HHV-6 causes a primary infection usually during the first two-three years of life, after which it becomes dormant predominantly inside monocytes and macrophages.
“Similar to all herpesviruses, HHV-6 causes a primary infection, usually during the first two-three years of life, after which it becomes dormant predominantly inside monocytes and macrophages [3,4,5].”
From Human Herpesvirus 6—A Rare Aetiologic Agent for CNS Infections in Immunocompetent Individuals or an Underestimation? (2024) · cites this paper as [5]
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Metadata
- Authors
- Andre-Garnier, E., Milpied, N., Boutolleau, D., Saiagh, S., Billaudel, S., & Imbert-Marcille, B.-M.
- Affiliations
- Virology Laboratory, UPRES-JE 2437, Nantes University Hospital and UFR Sciences Pharmaceutiques, 9 quai Moncousu, 44093 Nantes Cedex, France
- Centre Hospitalier Universitaire de Nantes (FR)
- Pitié-Salpêtrière Hospital (FR)
- Cell and Gene Therapy Unit, Nantes University Hospital, France
- Venue
- Journal of General Virology
- Publisher
- Microbiology Society
- Published
- 2004-11-01
- Volume
- 85
- Issue
- 11
- Pages
- 3333-3336
- ISSN
- 0022-1317 (ISSN-L)
- 1465-2099
- OpenAlex
- W2171021556
- Cited by
- 27
- References
- 18
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 4 · 2014: 1 · 2013: 1 · 2012: 1
- FWCI
- 1.20
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of General Virology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Epidemiology › Cytomegalovirus and herpesvirus research
- Health Sciences › Medicine › Oncology › Viral-associated cancers and disorders
- Health Sciences › Medicine › Infectious Diseases › Herpesvirus Infections and Treatments
- Keywords
- human herpesvirus 6, viral reactivation, ex vivo expansion, peripheral blood progenitor cells, viral replication, myeloid differentiation, interleukin-1, stem cell factor, granulocyte colony-stimulating factor
- MeSH terms
- Cells, Cultured, Female, Hematopoietic Stem Cells, Humans, Male, Middle Aged, RNA Splicing, RNA, Messenger, RNA, Viral, Virus Activation, Herpesvirus 6, Human, Antigens, CD34, Roseolovirus Infections, Viral Load
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