The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
UNOISE2: improved error-correction for Illumina 16S and ITS amplicon sequencing
Open accessAbstract
Amplicon sequencing of tags such as 16S and ITS ribosomal RNA is a popular method for investigating microbial populations. In such experiments, sequence errors caused by PCR and sequencing are difficult to distinguish from true biological variation. I describe UNOISE2, an updated version of the UNOISE algorithm for denoising (error-correcting) Illumina amplicon reads and show that it has comparable or better accuracy than DADA2.
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DADA2: High–resolution sample inference from Illumina amplicon data
Callahan, B. J., McMurdie, P. J., Rosen, M. J., Han, A. W., Johnson, A. J. A., & Holmes, S. P. (2016) · Nature Methods · doi:10.1038/nmeth.3869 · Cited by 35,698
A framework for human microbiome research
The Human Microbiome Project Consortium (2012) · Nature · doi:10.1038/nature11209 · Cited by 2,650
Huang, H.-L., Charoenkwan, P., Kao, T.-F., Lee, H.-C., Chang, F.-L., Huang, W.-L., Ho, S.-J., Shu, L.-S., Chen, W.-L., & Ho, S.-Y. (2012) · BMC Bioinformatics · doi:10.1186/1471-2105-13-s17-s3 · Cited by 88
Research works printed but not linked: 23
- 10.1128/JB.186.9.2629-2635.2004 · DOI · Paper page
- 10.1038/nmeth.2276 · DOI · Paper page
- 10.1016/j.funeco.2012.06.003 · DOI · Paper page
- Edgar,R.C. (2016) UCHIME2: improved chimera prediction for amplicon sequencing. http://dx.doi.org/10.1101/074252 · DOI · Paper page
- 10.1093/bioinformatics/btq461 · DOI · Paper page
- 10.1093/bioinformatics/btr381 · DOI · Paper page
- 10.1038/nmeth.2604 · DOI · Paper page
- 10.1038/ismej.2014.195 · DOI · Paper page
- Haas,B. et al. (2011) Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons. Genome Res., 494–504. · DOI · Paper page
- 10.1111/mec.12481 · DOI · Paper page
- 10.1128/AEM.01043-13 · DOI · Paper page
- 10.1038/nature11237 · DOI · Paper page
- 10.1038/srep08988 · DOI · Paper page
- 10.1093/nar/gkm864 · DOI · Paper page
- 10.1038/nmeth.1361 · DOI · Paper page
- 10.1186/1471-2105-12-38 · DOI · Paper page
- 10.1038/nmeth0910-668b · DOI · Paper page
- 2013, Denoising PCR-amplified metagenome data, BMC Bioinformatics, 13, 1
- Schirmer,M. et al. (2015) Insight into biases and sequencing errors for amplicon sequencing with the Illumina MiSeq platform. Nucleic Acids Res., 43. · DOI · Paper page
- 10.1128/AEM.01541-09 · DOI · Paper page
- 10.1016/j.soilbio.2013.05.014 · DOI · Paper page
- 10.1038/ismej.2014.117 · DOI · Paper page
- 10.1073/pnas.1002355107 · DOI · Paper page
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Cascone, T., William, W. N., Weissferdt, A., Leung, C. H., Lin, H. Y., Pataer, A., Godoy, M. C. B., Carter, B. W., Federico, L., Reuben, A., et al. (2021) · Nature Medicine · doi:10.1038/s41591-020-01224-2 · Cited by 618
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Metadata
- Authors
- Edgar, R. C.
- Venue
- bioRxiv (Cold Spring Harbor Laboratory)
- Publisher
- openRxiv
- Published
- 2016-10-15
- OpenAlex
- W2538509698
- Cited by
- 1,809
- References
- 3
- Type
- posted content
- Language
- en
- Open access
- green
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