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PreprintCited by 112Genomics and Chromatin DynamicsDNA Repair MechanismsDiffusion and Search Dynamics

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A hold-and-feed mechanism drives directional DNA loop extrusion by condensin

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Shaltiel, Indra A., Datta, Sumanjit, Lecomte, Léa, Hassler, Markus, Kschonsak, Marc, Bravo, Sol, Stober, Catherine, Eustermann, Sebastian, Haering, Christian H.·2021·Science
DOI 10.1101/2021.10.29.466147

Preprint — published as 10.1126/science.abm4012 · Paper page

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Abstract

SMC protein complexes structure genomes by extruding DNA loops, but the molecular mechanism that underlies their activity has remained unknown. We show that the active condensin complex entraps the bases of a DNA loop in two separate chambers. Single-molecule and cryo-electron microscopy provide evidence for a power-stroke movement at the first chamber that feeds DNA into the SMC-kleisin ring upon ATP binding, while the second chamber holds on upstream of the same DNA double helix. Unlocking the strict separation of ‘motor’ and ‘anchor’ chambers turns condensin from a one-sided into a bidirectional DNA loop extruder. We conclude that the orientation of two topologically bound DNA segments during the course of the SMC reaction cycle determines the directionality of DNA loop extrusion.

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Research works printed but not linked: 53

  1. I. F. Davidson , J. M. Peters , Genome folding through loop extrusion by SMC complexes. Nat Rev Mol Cell Biol, (2021). · DOI
  2. 2019, Organization of Chromosomal DNA by SMC Complexes, Annu Rev Genet, 53, 445, 10.1146/annurev-genet-112618-043633 · DOI
  3. 2021, On the choreography of genome folding: A grand pas de deux of cohesin and CTCF, Curr Opin Cell Biol, 70, 84, 10.1016/j.ceb.2020.12.001 · DOI
  4. 2017, Capturing condensin in chromosomes, Nat Genet, 49, 1419, 10.1038/ng.3962 · DOI
  5. J. H. Gibcus et al., A pathway for mitotic chromosome formation. Science 359, (2018). · DOI
  6. 10.1126/science.aar7831 · DOI · Paper page
  7. 10.1126/science.aaz3418 · DOI · Paper page
  8. 10.1126/science.aaz4475 · DOI · Paper page
  9. S. Golfier , T. Quail , H. Kimura , J. Brugues , Cohesin and condensin extrude DNA loops in a cell cycle-dependent manner. Elife 9, (2020). · DOI
  10. 10.1016/S1097-2765(02)00515-4 · DOI
  11. 10.1038/sj.emboj.7601562 · DOI
  12. 10.1016/j.cell.2017.09.008 · DOI · Paper page
  13. Y. Li et al., Structural basis for Scc3-dependent cohesin recruitment to chromatin. Elife 7, (2018). · DOI
  14. 10.1038/nsmb.2831 · DOI
  15. 10.1126/science.abb0981 · DOI
  16. 10.1016/j.molcel.2020.07.013 · DOI
  17. J. E. Collier et al., Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3. Elife 9, (2020). · DOI
  18. 10.1016/j.molcel.2019.03.037 · DOI
  19. 10.1016/j.cbpa.2011.07.017 · DOI
  20. 10.1038/35037523 · DOI
  21. 2019, Mechanism of DNA End Sensing and Processing by the Mre11-Rad50 Complex, Mol Cell, 76, 382, 10.1016/j.molcel.2019.07.035 · DOI
  22. 10.1016/j.sbi.2016.01.006 · DOI
  23. 10.1016/j.molcel.2006.06.019 · DOI
  24. 10.1126/science.1256917 · DOI
  25. 10.1038/nature07098 · DOI · Paper page
  26. 10.1016/j.cell.2018.04.015 · DOI
  27. 10.1038/nsmb.2087 · DOI · Paper page
  28. 2020, Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism, Nat Struct Mol Biol, 27, 743, 10.1038/s41594-020-0457-x · DOI
  29. K. Hara et al., Structural basis of HEAT-kleisin interactions in the human condensin I subcomplex. EMBO Rep 20, (2019). · DOI
  30. 2020, DNA-loop extruding condensin complexes can traverse one another, Nature, 579, 438, 10.1038/s41586-020-2067-5 · DOI
  31. 10.1016/j.cub.2018.08.034 · DOI
  32. 10.1093/nar/gkz497 · DOI
  33. 10.1016/j.molcel.2017.06.010 · DOI
  34. 10.1038/s41594-019-0196-z · DOI
  35. B. Pradhan et al., SMC complexes can traverse physical roadblocks bigger than their ring size. bioRxiv, 2021.2007.2015.452501 (2021). · DOI
  36. T. L. Higashi , G. Pobegalov , M. Tang , M. I. Molodtsov , F. Uhlmann , A Brownian ratchet model for DNA loop extrusion by the cohesin complex. Elife 10, (2021). · DOI
  37. 10.1038/s41586-019-1910-z · DOI · Paper page
  38. 10.1126/science.aan6516 · DOI
  39. 10.1038/nmeth983 · DOI · Paper page
  40. 10.1038/nmeth.2019 · DOI · Paper page
  41. 2019, Software tools for automated transmission electron microscopy, Nat Methods, 16, 471, 10.1038/s41592-019-0396-9 · DOI
  42. J. Zivanov et al., New tools for automated high-resolution cryo-EM structure determination in RELION-3. Elife 7, (2018). · DOI
  43. 10.1038/nmeth.4169 · DOI · Paper page
  44. 10.1038/nmeth.4193 · DOI
  45. 10.1016/j.jsb.2015.08.008 · DOI
  46. 2019, Real-time cryo-electron microscopy data preprocessing with Warp, Nat Methods, 16, 1146, 10.1038/s41592-019-0580-y · DOI
  47. 2019, Positive-unlabeled convolutional neural networks for particle picking in cryo-electron micrographs, Nat Methods, 16, 1153, 10.1038/s41592-019-0575-8 · DOI
  48. 10.1016/j.jmb.2003.07.013 · DOI · Paper page
  49. 10.1002/PRO.3943 · DOI
  50. 10.1107/S0907444910007493 · DOI · Paper page
  51. 10.1107/S2059798318002425 · DOI
  52. 10.1107/S2059798318006551 · DOI
  53. 1995, Statistical mechanics of supercoiled DNA, Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics, 52, 2912

Metadata

Authors
Shaltiel, I. A., Datta, S., Lecomte, L., Hassler, M., Kschonsak, M., Bravo, S., Stober, C., Eustermann, S., & Haering, C. H.
Affiliations
  • Heidelberg University (DE)
  • University of Würzburg (DE)
  • European Molecular Biology Laboratory (DE)
Corresponding author
  • Datta, Sumanjit
  • Lecomte, Léa
  • Eustermann, Sebastian
  • Haering, Christian H.
ORCID
Venue
Science
Publisher
openRxiv
Published
2021-10-29
Volume
376
Issue
6597
Pages
1087-1094
ISSN
0036-8075 (ISSN-L)
DOI
10.1101/2021.10.29.466147
OpenAlex
W4281793572
Cited by
112
References
1
Citations by year
2026: 18 · 2025: 24 · 2024: 24 · 2023: 27 · 2022: 19
FWCI
7.68
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Science (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek
  • Darwin Trust of Edinburgh
  • H2020 European Research Council (681365)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Diffusion and Search Dynamics
Keywords
condensin, DNA loop extrusion, structural maintenance of chromosomes, power stroke, ATP binding, chromosome organization, single-molecule imaging, cryo-electron microscopy
MeSH terms
Single Molecule Imaging, Adenosine Triphosphatases, DNA, DNA-Binding Proteins, Nucleic Acid Conformation, Cryoelectron Microscopy, Multiprotein Complexes

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