CoChat
Sign Up
PreprintCited by 8Nonlinear Optical Materials StudiesOptical Imaging and Spectroscopy TechniquesLaser Material Processing Techniques

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Precision 1070 nm Ultrafast Laser-induced Photothrombosis (PLP) of Depth-targeted Vessels in vivo

Open access
Zhu, Liang, Wang, Mengqi, Fu, Peng, Liu, Yin, Zhang, Hequn, Roe, Anna Wang, Xi, Wang·2022·Small Methods
DOI 10.1101/2022.06.22.497150

Preprint — published as 10.1002/smtd.202200917 · Paper page

Open this paper in CoChat — free account

Abstract

The cerebrovasculature plays an essential role in neurovascular and homeostatic functions in health and disease conditions. Many efforts have been made for developing vascular thrombosis methods to study vascular dysfunction in vivo , while technical challenges remain, such as accuracy and depth-selectivity to target single vessel in the cerebral cortex. Herein, we first demonstrates the evaluation and quantification of the feasibility and effects of Rose Bengal (RB)-induced photothrombosis with 720-1070 nm ultrafast lasers in raster scan. A flexible and reproducible approach is then proposed to employ a 1070 nm ultrafast laser with spiral scan for producing RB-induced occlusion, which is described as precision ultrafast laser-induced photothrombosis (PLP). Combine with two-photon microscopy imaging, this PLP displays highly precise and fast occlusion induction of various vessel types, sizes, and depths, which enhances the precision and power of the photothrombosis protocol. Overall, the PLP method provides a real-time, practical, precise, and depth-selected single-vessel photothrombosis technology in cerebral cortex with commercially available optical equipment, which is crucial for exploring brain vascular function with high spatial temporal resolution in brain.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/2022.06.22.497150

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Research works printed but not linked: 47

  1. 2017, The Neurovascular Unit Coming of Age: A Journey through Neurovascular Coupling in Health and Disease, Neuron, 96, 17, 10.1016/j.neuron.2017.07.030 · DOI · Paper page
  2. 10.1016/j.neuron.2013.10.008 · DOI · Paper page
  3. 10.1152/japplphysiol.00966.2005 · DOI
  4. 10.1038/nn.3426 · DOI · Paper page
  5. 2020, Mapping the Fine-Scale Organization and Plasticity of the Brain Vasculature, Cell, 180, 780, 10.1016/j.cell.2020.01.028 · DOI
  6. 2021, Brain microvasculature has a common topology with local differences in geometry that match metabolic load, Neuron, 109, 1168, 10.1016/j.neuron.2021.02.006 · DOI
  7. Chen, B. R ., Bouchard, M. B ., McCaslin, A. F ., Burgess, S. A . & Hillman, E. M . High-speed vascular dynamics of the hemodynamic response. Neuroimage 54 (2011). · DOI
  8. 10.1038/sj.bmt.1701832 · DOI
  9. 10.1146/annurev-neuro-071013-014111 · DOI
  10. 2018, Linking brain vascular physiology to hemodynamic response in ultra-high field MRI, Neuroimage, 168, 279, 10.1016/j.neuroimage.2017.02.063 · DOI
  11. 10.1038/324361a0 · DOI
  12. 2017, Intrinsic signal optical imaging of visual brain activity: Tracking of fast cortical dynamics, Neuroimage, 148, 160, 10.1016/j.neuroimage.2017.01.006 · DOI
  13. 10.1038/s41593-018-0234-x · DOI · Paper page
  14. 10.1038/s41593-018-0329-4 · DOI · Paper page
  15. 10.1038/s41582-019-0281-2 · DOI · Paper page
  16. 10.1016/j.neuron.2008.01.003 · DOI · Paper page
  17. 10.1038/35084005 · DOI · Paper page
  18. 10.1038/nature07664 · DOI · Paper page
  19. 2007, Acute ischemic stroke: Overview of major experimental rodent models, pathophysiology, and therapy of focal cerebral ischemia, Pharmacol Biochem Be, 87, 179, 10.1016/j.pbb.2007.04.015 · DOI
  20. 10.1161/01.STR.20.1.84 · DOI · Paper page
  21. 10.1038/sj.jcbfm.9600591 · DOI
  22. 10.1016/j.neuron.2014.08.052 · DOI
  23. 2017, Control of cerebral ischemia with magnetic nanoparticles, Nat Methods, 14, 160, 10.1038/nmeth.4105 · DOI
  24. 10.1007/s004150050021 · DOI
  25. 10.1038/nmeth844 · DOI · Paper page
  26. 10.1126/sciadv.aan9388 · DOI
  27. 10.1523/JNEUROSCI.1085-05.2005 · DOI · Paper page
  28. 10.1002/ana.410170513 · DOI · Paper page
  29. 10.1161/hs0202.102730 · DOI
  30. 10.1016/j.celrep.2018.06.119 · DOI
  31. 2022, Depth-targeted intracortical microstroke by two-photon photothrombosis in rodent brain, Neurophotonics, 9, 021910
  32. 10.1016/j.cmet.2015.10.010 · DOI · Paper page
  33. Roche, M . et al. In vivo imaging with a water immersion objective affects brain temperature, blood flow and oxygenation. Elife 8 (2019). · DOI
  34. 10.3389/fphys.2017.00979 · DOI
  35. Wu, X . et al. Tether-free photothermal deep-brain stimulation in freely behaving mice via wide-field illumination in the near-infrared-II window. Nat Biomed Eng (2022). · DOI · Paper page
  36. Horton, N. G . et al. In vivo three-photon microscopy of subcortical structures within an intact mouse brain. Nat Photonics 7 (2013). · DOI · Paper page
  37. 10.1088/0022-3727/38/15/004 · DOI · Paper page
  38. 10.1371/journal.pone.0104250 · DOI
  39. Linz, N ., Freidank, S ., Liang, X.-X . & Vogel, A . Wavelength dependence of femtosecond laser-induced breakdown in water and implications for laser surgery. Physical Review B 94 (2016). · DOI
  40. 10.1016/S0006-3495(01)76173-5 · DOI · Paper page
  41. 2005, Mechanisms of femtosecond laser nanosurgery of cells and tissues, Applied Physics B, 81, 1015, 10.1007/s00340-005-2036-6 · DOI
  42. 10.1016/S1474-4422(10)70104-6 · DOI · Paper page
  43. 10.1016/S1474-4422(13)70060-7 · DOI · Paper page
  44. Zhu, L . et al. Imaging microvasculature network evolution and neurodegeneration with precise photothrombosis approach. bioRxiv, 2021.2011.2029.470313 (2021). · DOI
  45. 10.1038/nprot.2014.165 · DOI
  46. 10.1371/journal.pone.0038590 · DOI
  47. 2021, VasoMetrics: unbiased spatiotemporal analysis of microvascular diameter in multi-photon imaging applications, Quant Imaging Med Surg, 11, 969

Metadata

Authors
Zhu, L., Wang, M., Fu, P., Liu, Y., Zhang, H., Roe, A. W., & Xi, W.
Affiliations
Second Affiliated Hospital of Zhejiang University (CN)
ORCID
Venue
Small Methods
Publisher
openRxiv
Published
2022-06-26
Volume
7
Issue
1
Pages
e2200917-e2200917
ISSN
2366-9608 (ISSN-L)
DOI
10.1101/2022.06.22.497150
OpenAlex
W4307340620
Cited by
8
References
1
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 1
FWCI
0.44
Type
journal article
Language
en
Open access
Licence
cc-by-nc-nd
Locations
  • Small Methods (publishedVersion, cc-by-nc-nd)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (U20A2022‐1, 91632105, 81961128029, 2018YFA0701400)
  • National Key Research and Development Program of China (2018YFA0701400)
  • Fundamental Research Funds for the Central Universities (226–2022-00083, 2019XZZX003–20, 2019XZZX003)
  • Key Research and Development Program of Zhejiang Province (2020C03004)
Topics
  • Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Nonlinear Optical Materials Studies
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Optical Imaging and Spectroscopy Techniques
  • Physical Sciences › Engineering › Industrial and Manufacturing Engineering › Laser Material Processing Techniques
Keywords
photothrombosis, rose bengal, cerebral cortex, spiral scan, two-photon microscopy, vascular dysfunction, cerebrovascular function
MeSH terms
Brain, Humans, Lasers, Rose Bengal, Thrombosis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review