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PreprintCited by 53Bacteriophages and microbial interactionsAdvanced Electron Microscopy Techniques and ApplicationsEnzyme Structure and Function

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Cryo-electron microscopy of the f1 filamentous phage reveals a new paradigm in viral infection and assembly

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Conners, Rebecca, León-Quezada, Rayén Ignacia, McLaren, Mathew, Bennett, Nicholas J, Daum, Bertram, Rakonjac, Jasna, Gold, Vicki A M·2022·Nature Communications
DOI 10.1101/2022.11.03.514279PMC PMC10175506

Preprint — published as 10.1038/s41467-023-37915-w

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Abstract

Phages are viruses that infect bacteria and dominate every ecosystem on our planet. As well as impacting microbial ecology, physiology and evolution, phages are exploited as tools in molecular biology and biotechnology. This is particularly true for the Ff (f1, fd or M13) phages, which represent a widely distributed group of filamentous viruses. Over nearly five decades, Ff has seen an extraordinary range of applications, including in phage display and nanotechnology. However, the complete structure of the phage capsid and consequently the mechanisms of infection and assembly remain largely mysterious. Using cryo-electron microscopy and a highly efficient system for production of short Ff-derived nanorods, we have determined the first structure of a filamentous virus, including the filament tips. Structure combined with mutagenesis was employed to identify domains of the phage that are important in bacterial attack and for release of new phage progeny. These data allow new models to be proposed for the phage lifecycle and will undoubtedly enable the development of novel biotechnological applications.

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Metadata

Authors
Conners, R., León-Quezada, R. I., McLaren, M., Bennett, N. J., Daum, B., Rakonjac, J., & Gold, V. A. M.
Affiliations
  • University of Exeter (GB)
  • Massey University (NZ)
Corresponding author
  • Rakonjac, Jasna
  • Gold, Vicki A M
ORCID
Venue
Nature Communications
Publisher
openRxiv
Published
2022-11-04
Volume
14
Issue
1
Pages
2724-2724
ISSN
2041-1723 (ISSN-L)
DOI
10.1101/2022.11.03.514279
PMC
PMC10175506
OpenAlex
W4376225524
Cited by
53
References
1
Citations by year
2026: 9 · 2025: 17 · 2024: 20 · 2023: 7
FWCI
8.57
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • University of Missouri
  • Callaghan Innovation
  • Wellcome Trust (206181/Z/17/Z, 206181, Z/17/Z, 210363/Z/18/Z, 202904, 210363, 202904/Z/16/Z)
  • Diamond Light Source
  • University of Exeter
  • European Commission (HORIZON2020, 803894)
  • Massey University
  • Medical Research Council
  • Biotechnology and Biological Sciences Research Council (BB/R000484/1, BB/R008639/1)
Topics
  • Life Sciences › Immunology and Microbiology › Virology › Bacteriophages and microbial interactions
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Advanced Electron Microscopy Techniques and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Structural Biology › Enzyme Structure and Function
Keywords
phage assembly, phage infection mechanism, nanorods, bacteriophages
MeSH terms
Bacteria, Bacteriophages, Humans, Virus Diseases, Ecosystem, Inovirus, Cryoelectron Microscopy

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