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PreprintCited by 33Photosynthetic Processes and MechanismsAlgal biology and biofuel productionLight effects on plants

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Systematic identification and characterization of novel genes in the regulation and biogenesis of photosynthetic machinery

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Kafri, Moshe, Patena, Weronika, Martin, Lance, Wang, Lianyong, Gomer, Gillian, Sirkejyan, Arthur K, Goh, Audrey, Wilson, Alexandra T., Gavrilenko, Sophia E, Breker, Michal, Roichman, Asael, McWhite, Claire D., Rabinowitz, Joshua D., Cross, Frederick R, Wühr, Martin, Jonikas, Martin C.·2022·Cell
DOI 10.1101/2022.11.12.515357PMC PMC10760936

Preprint — published as 10.1016/j.cell.2023.11.007 · Paper page

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Abstract

SUMMARY: Photosynthesis is central to food production and the Earth’s biogeochemistry, yet the molecular basis for its regulation remains poorly understood. Here, using high-throughput genetics in the model eukaryotic alga Chlamydomonas reinhardtii , we identify with high confidence (FDR<0.11) 70 previously-uncharacterized genes required for photosynthesis. We then provide a resource of mutant proteomes that enables functional characterization of these novel genes by revealing their relationship to known genes. The data allow assignment of 34 novel genes to the biogenesis or regulation of one or more specific photosynthetic complexes. Additional analysis uncovers at least seven novel critical regulatory proteins, including five Photosystem I mRNA maturation factors and two master regulators: MTF1, which impacts chloroplast gene expression directly; and PMR1, which impacts expression via nuclear-expressed factors. Our work provides a rich resource identifying novel regulatory and functional genes and placing them into pathways, thereby opening the door to a system-level understanding of photosynthesis. Highlights: High-confidence identification of 70 previously-uncharacterized genes required for photosynthesis Proteomic analysis of mutants allows assignment of function to novel genes Characterization of 5 novel Photosystem I mRNA maturation factors validates this resource MTF1 and PMR1 identified as master regulators of photosynthesis

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Metadata

Authors
Kafri, M., Patena, W., Martin, L., Wang, L., Gomer, G., Sirkejyan, A. K., Goh, A., Wilson, A. T., Gavrilenko, S. E., Breker, M., Roichman, A., McWhite, C. D., Rabinowitz, J. D., Cross, F. R., Wühr, M., & Jonikas, M. C.
Affiliations
  • Princeton University (US)
  • Rockefeller University (US)
  • Howard Hughes Medical Institute (US)
Corresponding author
Jonikas, Martin C.
ORCID
Venue
Cell
Publisher
openRxiv
Published
2022-11-15
Volume
186
Issue
25
Pages
5638-5655.e25
ISSN
0092-8674 (ISSN-L)
DOI
10.1101/2022.11.12.515357
PMC
PMC10760936
OpenAlex
W4389427712
Cited by
33
References
2
Citations by year
2026: 9 · 2025: 14 · 2024: 10
FWCI
2.93
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Cell (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion, cc-by)
Funders
  • Howard Hughes Medical Institute (55108535)
  • Simons Foundation (55108535)
  • European Molecular Biology Organization (ALTF 1006-2017)
  • Princeton University
  • Horowitz Foundation for Social Policy
  • Human Frontier Science Program
  • Natural Resources, Energy and Science Authority of Sri Lanka (MCB-1914989)
  • National Institutes of Health
  • National Institute of General Medical Sciences (R35GM128813)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
Keywords
photosynthesis, photosynthesis regulation, Chlamydomonas reinhardtii, gene identification, MTF-1, PMR1, photosynthetic complexes
MeSH terms
Chloroplasts, Gene Expression Regulation, Mutation, Photosynthesis, Proteins, Ribosomes, RNA, Messenger, Chlamydomonas reinhardtii

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