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PreprintCited by 18Genetic and Kidney Cyst DiseasesMicrotubule and mitosis dynamicsCystic Fibrosis Research Advances

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MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement

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Tran, Michael V., Ferguson, James W., Cote, Lauren E., Khuntsariya, Daria, Fetter, Richard D., Wang, Jennifer T., Wellard, Stephen R., Sallee, Maria D., Genova, Mariya, Eskinazi, Sani, Magiera, Maria M., Janke, Carsten, Stearns, Tim, Lansky, Zdenek, Shen, Kang, Magescas, Jérémy, Feldman, Jessica L.·2023·Developmental Cell
DOI 10.1101/2023.02.23.529616PMC PMC11385174

Preprint — published as 10.1016/j.devcel.2023.12.001 · Paper page

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Abstract

Summary: Microtubule doublets (MTDs) are a well conserved compound microtubule structure found primarily in cilia. However, the mechanisms by which MTDs form and are maintained in vivo remain poorly understood. Here, we characterize microtubule-associated protein 9 (MAP9) as a novel MTD-associated protein. We demonstrate that C. elegans MAPH-9, a MAP9 homolog, is present during MTD assembly and localizes exclusively to MTDs, a preference that is in part mediated by tubulin polyglutamylation. Loss of MAPH-9 caused ultrastructural MTD defects, dysregulated axonemal motor velocity, and perturbed cilia function. As we found that the mammalian ortholog MAP9 localized to axonemes in cultured mammalian cells and mouse tissues, we propose that MAP9/MAPH-9 plays a conserved role in supporting the structure of axonemal MTDs and regulating ciliary motors.

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  1. 10.1016/j.tcb.2019.07.004 · DOI
  2. 10.1111/j.1365-2818.2008.01984.x · DOI
  3. 10.1016/j.devcel.2007.03.004 · DOI
  4. 10.1083/jcb.200908133 · DOI
  5. 10.1242/bio.017442 · DOI
  6. 2021, Microtubule-associated proteins and emerging links to primary cilium structure, assembly, maintenance, and disassembly, FEBS J, 288, 786, 10.1111/febs.15473 · DOI
  7. 10.1111/nep.12022 · DOI
  8. 10.1534/genetics.115.178335 · DOI
  9. 10.1126/science.1152993 · DOI · Paper page
  10. 10.7554/eLife.01948 · DOI
  11. 10.1091/mbc.E02-04-0205 · DOI
  12. 2017, She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domain, Nat Commun, 8, 2151, 10.1038/s41467-017-02004-2 · DOI
  13. 10.1126/science.1967194 · DOI
  14. 10.1186/1471-2105-5-113 · DOI
  15. 10.1083/jcb.200509115 · DOI
  16. 10.1038/ncb1877 · DOI · Paper page
  17. 10.4161/cc.27944 · DOI
  18. 10.1007/s00335-016-9627-x · DOI
  19. 10.1242/jcs.199471 · DOI
  20. 10.1016/j.cub.2021.03.023 · DOI
  21. 10.1016/j.cell.2012.10.038 · DOI
  22. 10.1093/sysbio/syq010 · DOI · Paper page
  23. 10.1038/ncb2268 · DOI
  24. 10.1371/journal.pgen.1006469 · DOI
  25. 10.1038/s41594-020-0507-4 · DOI
  26. 10.1074/jbc.C110.128280 · DOI
  27. 10.1242/jcs.185983 · DOI
  28. 10.1093/molbev/msx149 · DOI
  29. 10.15252/embj.201796883 · DOI
  30. 10.15252/embj.201592929 · DOI
  31. 10.1523/JNEUROSCI.1746-04.2004 · DOI
  32. 10.1016/0896-6273(95)90242-2 · DOI
  33. 2018, MAP9 single nucleotide polymorphism rs1058992 is a risk of EBV-associated gastric carcinoma in Chinese population, Acta Virol, 62, 435, 10.4149/av_2018_412 · DOI
  34. 10.1016/j.cub.2021.03.022 · DOI
  35. 10.1042/BCJ20170453 · DOI
  36. 10.1038/s41467-018-03909-2 · DOI · Paper page
  37. 10.1016/j.devcel.2020.01.029 · DOI · Paper page
  38. 10.1091/mbc.E08-11-1115 · DOI
  39. Nechipurenko, I. V. , Berciu, C. , Sengupta, P. , & Nicastro, D. (2017). Centriolar remodeling underlies basal body maturation during ciliogenesis in Caenorhabditis elegans. Elife, 6. https://doi.org/10.7554/eLife.25686 · DOI
  40. 10.1126/science.1128618 · DOI
  41. 2021, Expression Patterns and Levels of All Tubulin Isotypes Analyzed in GFP Knock-In C. elegans Strains, Cell Struct Funct, 46, 51, 10.1247/csf.21022 · DOI
  42. 10.1038/s41467-019-09051-x · DOI · Paper page
  43. 10.1016/0012-1606(86)90314-3 · DOI · Paper page
  44. 10.1016/j.jsb.2012.02.012 · DOI
  45. 10.1038/ncb3263 · DOI · Paper page
  46. 10.1073/pnas.0500964102 · DOI
  47. 10.1093/oxfordjournals.jmicro.a049610 · DOI
  48. 10.1126/science.aav2567 · DOI
  49. 10.1146/annurev-cellbio-101512-122335 · DOI
  50. 2022, Microtubule lattice spacing governs cohesive envelope formation of tau family proteins, Nat Chem Biol, 18, 1224, 10.1038/s41589-022-01096-2 · DOI · Paper page
  51. 10.1038/nature04816 · DOI
  52. 1974, The DNA of Caenorhabditis elegans, Genetics, 77, 95, 10.1093/genetics/77.1.95 · DOI
  53. 2021, Self-repair protects microtubules from destruction by molecular motors, Nat Mater, 20, 883, 10.1038/s41563-020-00905-0 · DOI · Paper page
  54. 10.1093/NAR/GKAB1061 · DOI · Paper page
  55. 10.1186/1471-2164-9-406 · DOI
  56. 10.1046/j.1365-2818.2002.01064.x · DOI
  57. Wang, J. T. , Kong, D. , Hoerner, C. R. , Loncarek, J. , & Stearns, T. (2017). Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells. Elife, 6. https://doi.org/10.7554/eLife.29061 · DOI
  58. 10.1158/1078-0432.CCR-19-1611 · DOI
  59. 10.1002/cne.901600305 · DOI
  60. 10.1093/bioinformatics/btp033 · DOI
  61. 10.1038/ncomms12437 · DOI
  62. Wloga, D. , Joachimiak, E. , & Fabczak, H. (2017). Tubulin Post-Translational Modifications and Microtubule Dynamics. Int J Mol Sci, 18(10). https://doi.org/10.3390/ijms18102207 · DOI
  63. 10.1091/mbc.E13-08-0464 · DOI · Paper page
  64. 10.1074/jbc.R115.637447 · DOI
  65. 2022, MAP9 Exhibits Protumor Activities and Immune Escape toward Bladder Cancer by Mediating TGF-beta1 Pathway, J Oncol, 2022, 3778623

Metadata

Authors
Tran, M. V., Ferguson, J. W., Cote, L. E., Khuntsariya, D., Fetter, R. D., Wang, J. T., Wellard, S. R., Sallee, M. D., Genova, M., Eskinazi, S., Magiera, M. M., Janke, C., Stearns, T., Lansky, Z., Shen, K., Magescas, J., & Feldman, J. L.
Affiliations
  • Stanford University (US)
  • Czech Academy of Sciences (CZ)
  • Howard Hughes Medical Institute (US)
  • Centre National de la Recherche Scientifique (FR)
  • Stanford Medicine (US)
Corresponding author
  • Magescas, Jérémy
  • Feldman, Jessica L.
ORCID
Venue
Developmental Cell
Publisher
openRxiv
Published
2023-02-23
Volume
59
Issue
2
Pages
199-210.e11
ISSN
1534-5807 (ISSN-L)
DOI
10.1101/2023.02.23.529616
PMC
PMC11385174
OpenAlex
W4390414505
Cited by
18
References
1
Citations by year
2026: 3 · 2025: 7 · 2024: 6 · 2023: 2
FWCI
2.98
Type
journal article
Language
en
Open access
Locations
  • Developmental Cell (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion)
  • SSRN Electronic Journal (acceptedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • HAL (submittedVersion, other-oa)
  • PubMed Central (submittedVersion)
Funders
  • Howard Hughes Medical Institute
  • American Heart Association (20POST35210767, 903408)
  • Damon Runyon Cancer Research Foundation (DRG-2428-21)
  • European Molecular Biology Organization
  • Fondation Vaincre Alzheimer (FR‐16055p)
  • Agence Nationale de la Recherche (P40 OD010440, ANR-20-CE13-0011)
  • Grantová Agentura České Republiky (RVO 86652036, GX19-27477X)
  • Fondation pour la Recherche Médicale (DEQ20170336756, P40 OD010440)
  • Institut Curie
  • National Institutes of Health (1s10od012276-01, OD010440, P40OD010440)
  • National Institute of General Medical Sciences (K99GM131024, R35GM130286, F32GM142181, R00GM131024, T32GM007276, R15GM135886, K99GM135489, R01GM136902, R01GM133950, R15GM114727)
  • National Institute of Neurological Disorders and Stroke (R01NS082208)
  • National Center for Research Resources (1S10OD01227601)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
Keywords
doublet microtubules, axoneme, cilia, tubulin polyglutamylation, microtubule-associated proteins, Caenorhabditis elegans, ciliary ultrastructure

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