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PreprintCited by 2Protein Structure and DynamicsSARS-CoV-2 and COVID-19 Researchvaccines and immunoinformatics approaches

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Coronaviruses Spike glycoprotein endodomains: the sequence and structure-based comprehensive study

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Kumar, Prateek, Bhardwaj, Aparna, Mukherjee, Bodhidipra, Joshi, Richa, Giri, Rajanish·2023·Protein Science
DOI 10.1101/2023.08.16.553512PMC PMC10599102

Preprint — published as 10.1002/pro.4804 · Paper page

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Abstract

The flexibility of proteins makes them available to interact with many biomolecules in the cell. Specifically, such interactions in viruses help them to perform more functions despite having a smaller genome. Therefore, these flexible regions can be exciting and essential targets to be explored for their role in pathogenicity and therapeutic developments as they achieve essential interactions. In the continuation with our previous study on disordered analysis of SARS-CoV-2 spike cytoplasmic tail (CTR), or endodomain, here we have explored the disordered potential endodomains of six other coronaviruses using multiple bioinformatics approaches and molecular dynamics simulations. Based on the comprehensive analysis of its sequence and structural composition, we report the varying disorder propensity in endodomains of spike proteins of coronaviruses. The observations of this study may help to understand the importance of spike glycoprotein endodomain and creating therapeutic interventions against them.

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Metadata

Authors
Kumar, P., Bhardwaj, A., Mukherjee, B., Joshi, R., & Giri, R.
Affiliations
Indian Institute of Technology Mandi (IN)
Corresponding author
Giri, Rajanish
ORCID
Venue
Protein Science
Publisher
openRxiv
Published
2023-08-16
Volume
32
Issue
11
Pages
e4804-e4804
ISSN
0961-8368 (ISSN-L)
DOI
10.1101/2023.08.16.553512
PMC
PMC10599102
OpenAlex
W4387607370
Cited by
2
References
1
Citations by year
2025: 1 · 2023: 1
FWCI
0.18
Type
journal article
Language
en
Open access
Locations
  • Protein Science (publishedVersion)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Indian Council of Medical Research (52/04/2020/BIO/BMS, 58/6/2020/PHA/BMS)
  • Ministry of Education, India (CRG/2019/005603, SPARC/2018-2019/P37/SL, BT/11/IYBA/2018/06)
  • Indian Institute of Technology Mandi (BT/11/IYBA/2018/06, CRG/2019/005603)
  • Scheme for Promotion of Academic and Research Collaboration
  • Science and Engineering Research Board (BT/11/IYBA/2018/06, CRG/2019/005603)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
Keywords
cytoplasmic tail, intrinsically disordered regions, molecular dynamics simulation, SARS-CoV-2, therapeutic target
MeSH terms
SARS-CoV-2, Glycoproteins, Humans, Molecular Dynamics Simulation, Spike Glycoprotein, Coronavirus

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