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PreprintCited by 21Epigenetics and DNA MethylationPlant Molecular Biology ResearchGenomics and Chromatin Dynamics

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ACD15, ACD21 and SLN regulate accumulation and mobility of MBD6 to silence genes and transposable elements

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Boone, Brandon A., Ichino, Lucia, Wang, Shuya, Gardiner, Jason, Yun, Jaewon, Jami-Alahmadi, Yasaman, Sha, Jihui, Mendoza, Cristy P., Steelman, Bailey J., van Aardenne, Aliya, Kira-Lucas, Sophia, Trentchev, Isabelle, Wohlschlegel, James A., Jacobsen, Steven E.·2023·Science Advances
DOI 10.1101/2023.08.23.554494PMC PMC10651127

Preprint — published as 10.1126/sciadv.adi9036

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Abstract

DNA methylation mediates silencing of transposable elements and genes in part via recruitment of the Arabidopsis MBD5/6 complex, which contains the methyl-CpG-binding domain (MBD) proteins MBD5 and MBD6, and the J-domain containing protein SILENZIO (SLN). Here we characterize two additional complex members: α-crystalline domain containing proteins ACD15 and ACD21. We show that they are necessary for gene silencing, bridge SLN to the complex, and promote higher order multimerization of MBD5/6 complexes within heterochromatin. These complexes are also highly dynamic, with the mobility of complex components regulated by the activity of SLN. Using a dCas9 system, we demonstrate that tethering the ACDs to an ectopic site outside of heterochromatin can drive massive accumulation of MBD5/6 complexes into large nuclear bodies. These results demonstrate that ACD15 and ACD21 are critical components of gene silencing complexes that act to drive the formation of higher order, dynamic assemblies. One-Sentence Summary: Arabidopsis ACD21 and ACD15 drive accumulation of MBD5/6 complex silencing assemblies at methyl-CG sites and recruit SLN to maintain protein mobility in these assemblages.

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Metadata

Authors
Boone, B. A., Ichino, L., Wang, S., Gardiner, J., Yun, J., Jami-Alahmadi, Y., Sha, J., Mendoza, C. P., Steelman, B. J., van Aardenne, A., Kira-Lucas, S., Trentchev, I., Wohlschlegel, J. A., & Jacobsen, S. E.
Affiliations
  • University of California, Los Angeles (US)
  • Howard Hughes Medical Institute (US)
Corresponding author
Jacobsen, Steven E.
ORCID
Venue
Science Advances
Publisher
openRxiv
Published
2023-08-24
Volume
9
Issue
46
Pages
eadi9036-eadi9036
ISSN
2375-2548 (ISSN-L)
DOI
10.1101/2023.08.23.554494
PMC
PMC10651127
OpenAlex
W4388711621
Cited by
21
References
1
Citations by year
2026: 3 · 2025: 12 · 2024: 5 · 2023: 1
FWCI
1.87
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Science Advances (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • eScholarship (California Digital Library) (submittedVersion, cc-by)
  • PubMed Central (submittedVersion, cc-by-nc-nd)
Funders
  • Howard Hughes Medical Institute (R01 GM089778)
  • National Institutes of Health
  • National Institute of General Medical Sciences (R35GM130272, R01GM089778)
  • Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
DNA methylation, gene silencing, transposon silencing, heterochromatin, CG methylation, nuclear bodies, methyl-CpG binding domain proteins, Arabidopsis thaliana

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