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One-step N-terminomics based on isolation of protein N-terminal peptides from LysargiNase digests by tip-based strong cation exchange chromatography
Open accessPreprint — published as 10.1016/j.mcpro.2024.100820
Abstract
We have developed a one-step isolation method for protein N-terminal peptides from LysargiNase digests by pipette tip-based strong cation exchange (SCX) chromatography. This CHAMP-N (CHromatographic AMplification of Protein N-terminal peptides) method using disposable and parallel-processable SCX tips instead of conventional HPLC SCX columns facilitates simple, sensitive and high-throughput N-terminomic profiling without sacrificing the high identification numbers and selectivity achieved by the HPLC-based method. By applying the CHAMP-N method to HEK293T cells, we identified novel cleavage sites for signal and transit peptides, and non-canonical translation initiation sites. Finally, for proteome-wide terminomics, we present a simple and comprehensive N-and C-terminomics platform employing three different tip-based approaches, including CHAMP-N, in which protease digestion and one-step isolation by tip LC are commonly used to achieve complementary terminome coverages.
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Metadata
- Authors
- Morikawa, K., Nishida, H., Imami, K., & Ishihama, Y.
- Affiliations
- Kyoto University (JP)
- Corresponding author
- Ishihama, Yasushi
- Venue
- Molecular & Cellular Proteomics
- Publisher
- openRxiv
- Published
- 2024-02-07
- Volume
- 23
- Issue
- 9
- Pages
- 100820-100820
- ISSN
- 1535-9476 (ISSN-L)
- PMC
- PMC11382313
- OpenAlex
- W4401056525
- Cited by
- 10
- References
- 1
- Citations by year
- 2026: 5 · 2025: 5
- FWCI
- 1.25
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Molecular & Cellular Proteomics (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Japan Agency for Medical Research and Development (CREST, No. JP18gm1010010, JP18gm1010010)
- Japan Society for the Promotion of Science (21H02459, 23H04924, 23K18185, 20H03241, CREST)
- Core Research for Evolutional Science and Technology (JPMJCR1862, JP18gm1010010)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Physical Sciences › Chemistry › Organic Chemistry › Chemical Synthesis and Analysis
- Keywords
- N-terminomics, strong cation exchange chromatography, signal peptide cleavage site, proteolytic digestion
- MeSH terms
- Chromatography, Ion Exchange, Humans, Peptides, Proteome, Proteomics, HEK293 Cells
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