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A genus-specific nsp12 region impacts polymerase assembly in Alpha- and Gammacoronaviruses
Open accessPreprint — published as 10.1016/j.jbc.2024.107802
Abstract
Coronavirus relevancy for human health has surged over the past 20 years as they have a propensity for spillover into humans from animal reservoirs resulting in pandemics such as COVID-19. The diversity within the Coronavirinae subfamily and high infection frequency in animal species worldwide creates a looming threat that calls for research across all genera within the Coronavirinae subfamily. We sought to contribute to the limited structural knowledge within the Gammacoronavirus genera and determined the structure of the viral core replication-transcription complex (RTC) from Infectious Bronchitis Virus (IBV) using single-particle cryo-EM. Comparison between our IBV structure with published RTC structures from other Coronavirinae genera reveals structural differences across genera. Using in vitro biochemical assays, we characterized these differences and revealed their differing involvement in core RTC formation across different genera. Our findings highlight the value of cross-genera Coronavirinae studies, as they show genera specific features in coronavirus genome replication. A broader knowledge of coronavirus replication will better prepare us for future coronavirus spillovers.
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Highly accurate protein structure prediction with AlphaFold
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Metadata
- Authors
- Hoferle, P. J., Anderson, T. K., & Kirchdoerfer, R. N.
- Affiliations
- University of Wisconsin–Madison (US)
- Corresponding author
- Kirchdoerfer, Robert N.
- Venue
- Journal of Biological Chemistry
- Publisher
- openRxiv
- Published
- 2024-07-24
- Volume
- 300
- Issue
- 11
- Pages
- 107802-107802
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC11530588
- OpenAlex
- W4402669335
- Cited by
- 0
- References
- 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by-nc-nd)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- PubMed Central (submittedVersion, cc-by-nc-nd)
- Funders
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases (AI158463)
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases (AI158463, R01AI158463)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Keywords
- nsp12, RNA-dependent RNA polymerase, Gammacoronavirus, alphacoronavirus, infectious bronchitis virus
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