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PreprintCited by 11Microtubule and mitosis dynamicsProtein Structure and DynamicsGenetic and Kidney Cyst Diseases

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Mechanistic basis of temperature-adaptation in microtubule dynamics across frog species

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Troman, Luca, de Gaulejac, Ella, Biswas, Abin, Stiens, Jennifer, Kuropka, Benno, Moores, Carolyn, Reber, Simone·2024·Current Biology
DOI 10.1101/2024.07.29.605571

Preprint — published as 10.1016/j.cub.2024.12.022 · Paper page

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Abstract

SUMMARY: Cellular processes are remarkably effective across diverse temperature ranges, even with highly conserved proteins. In the context of the microtubule cytoskeleton, which is critically involved in a wide range of cellular activities, this is particularly striking as tubulin is one of the most conserved proteins while microtubule dynamic instability is highly temperature sensitive. We thus lack a mechanistic framework that links functional adaptability with environmental pressures. Here, we leverage the diversity of natural tubulin variants from three closely related frog species that live at different temperatures: we combine in vitro reconstitution assays, quantitative biochemistry, and cryogenic electron microscopy to show how a small number of primary sequence changes influences the energy landscape of tubulin interactions and thereby mediates cold-adaptation and microtubule stability. This study thus broadens our conceptual framework for understanding microtubule dynamics and provides insights into how conserved cellular processes are tailored to different ecological niches.

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Metadata

Authors
Troman, L., de Gaulejac, E., Biswas, A., Stiens, J., Kuropka, B., Moores, C., & Reber, S.
Affiliations
  • Institute of Structural and Molecular Biology (GB)
  • Humboldt-Universität zu Berlin (DE)
  • Marine Biological Laboratory (US)
  • Freie Universität Berlin (DE)
  • Berliner Hochschule für Technik (DE)
Corresponding author
  • Moores, Carolyn
  • Reber, Simone
ORCID
Venue
Current Biology
Publisher
openRxiv
Published
2024-07-29
Volume
35
Issue
3
Pages
612-628.e6
ISSN
0960-9822 (ISSN-L)
DOI
10.1101/2024.07.29.605571
OpenAlex
W4406235478
Cited by
11
References
1
Citations by year
2026: 6 · 2025: 5
FWCI
5.46
Type
journal article
Language
en
Open access
Licence
Locations
  • Current Biology (publishedVersion, cc-by-nc)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • BIROn (Birkbeck, University of London) (acceptedVersion)
  • Open Access Server of the Woods Hole Scientific Community (Woods Hole Scientific Community) (submittedVersion, cc-by-nc)
Funders
  • Wellcome Trust (202679/Z/16/Z, 206166/Z/17/Z)
  • Deutsche Forschungsgemeinschaft
  • Max-Planck-Gesellschaft
  • Medical Research Council (MR/Y000633/1, MR/R00352/1, MR/R000352/1)
  • Medical Research Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Structure and Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
Keywords
microtubule dynamics, thermal adaptation, tubulin, cold adaptation, frogs, microtubule structure, cytoskeleton
MeSH terms
Animals, Microtubules, Temperature, Tubulin, Cryoelectron Microscopy

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