The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Structural basis for a nucleoporin exportin complex between RanBP2, SUMO1-RanGAP1, the E2 Ubc9, Crm1 and the Ran GTPase
Open accessPreprint — published as 10.1038/s41467-025-61694-1 · Paper page
Abstract
The human nucleoporin RanBP2/Nup358 interacts with SUMO1-modified RanGAP1 and the SUMO E2 Ubc9 at the nuclear pore complex (NPC) to promote export and disassembly of exportin Crm1/Ran(GTP)/cargo complexes. In mitosis, RanBP2/SUMO1-RanGAP1/Ubc9 remains intact after NPC disassembly and is recruited to kinetochores and mitotic spindles by Crm1 where it contributes to mitotic progression. Interestingly, RanBP2 binds SUMO1-RanGAP1/Ubc9 via motifs that also catalyze SUMO E3 ligase activity. Here, we resolve cryo-EM structures of a RanBP2 C-terminal fragment in complex with Crm1, SUMO1-RanGAP1/Ubc9, and two molecules of Ran(GTP). These structures reveal several unanticipated interactions with Crm1 including a nuclear export signal (NES) for RanGAP1, the deletion of which mislocalizes RanGAP1 and the Ran GTPase in cells. Our structural and biochemical results support models in which RanBP2 E3 ligase activity is dependent on Crm1, the RanGAP1 NES and Ran GTPase cycling.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764
Flagged first, then by citations
Research works printed but not linked: 83
- Petrovic, S. , Mobbs, G.W. , Bley, C.J. , Nie, S. , Patke, A. & Hoelz, A . Structure and Function of the Nuclear Pore Complex. Cold Spring Harb Perspect Biol 14(2022). · DOI
- 10.1038/254109a0 · DOI
- 10.1016/S0006-3495(99)76883-9 · DOI
- 10.1093/emboj/cdg113 · DOI
- 2022, Karyopherin-mediated nucleocytoplasmic transport, Nature Reviews Molecular Cell Biology, 23, 307, 10.1038/s41580-021-00446-7 · DOI
- 10.1126/science.1068798 · DOI
- 1994, RanGAP1 induces GTPase activity of nuclear Ras-related Ran, Proc Natl Acad Sci U S A, 91, 2587-91
- 10.1038/354080a0 · DOI
- 10.1074/jbc.270.23.14209 · DOI
- 1995, Localization of the Ran-GTP binding protein RanBP2 at the cytoplasmic side of the nuclear pore complex, Eur J Cell Biol, 68, 211
- 10.1126/science.abm9129 · DOI
- 10.1002/1873-3468.14749 · DOI
- 10.1038/376184a0 · DOI
- 1998, SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex, J Cell Biol, 140, 499-509
- 10.1083/jcb.140.2.259 · DOI
- 10.1091/mbc.E07-12-1279 · DOI · Paper page
- 10.1038/ncomms11482 · DOI
- 10.1073/pnas.96.10.5516 · DOI
- 10.1073/pnas.94.8.3736 · DOI
- 10.1146/annurev.cellbio.22.010605.093503 · DOI
- 10.1146/annurev-biophys-051013-022958 · DOI
- 10.1021/acs.chemrev.6b00737 · DOI
- 10.1083/jcb.135.6.1457 · DOI
- 10.1074/jbc.273.11.6503 · DOI
- 10.1128/MCB.22.18.6498-6508.2002 · DOI
- 10.1016/S0092-8674(02)00630-X · DOI
- 10.1038/nature03588 · DOI
- 10.1016/S0092-8674(01)00633-X · DOI
- 10.1016/S0960-9822(98)70044-2 · DOI
- 10.1038/nsmb834 · DOI
- 10.1016/S0092-8674(01)00519-0 · DOI
- 10.1007/978-1-59745-566-4_1 · DOI
- 10.1038/nsmb.3116 · DOI
- 10.1038/nature11376 · DOI · Paper page
- 10.1016/j.molcel.2012.07.001 · DOI · Paper page
- 10.1038/nsmb.2621 · DOI
- 10.1016/j.molcel.2005.03.012 · DOI
- 10.1038/nsmb878 · DOI
- 10.1016/j.molcel.2009.02.008 · DOI
- 10.1074/jbc.M111.321141 · DOI
- 10.1091/mbc.E08-05-0511 · DOI
- 10.1016/j.cell.2008.01.045 · DOI
- 10.1016/j.molcel.2012.02.017 · DOI
- 10.1371/journal.pone.0141309 · DOI
- 10.1242/jcs.112.3.339 · DOI
- 10.1083/jcb.200110109 · DOI
- 10.1038/ncb1263 · DOI
- 10.1073/pnas.1217203110 · DOI
- 10.1126/science.1173388 · DOI
- 10.1038/nsmb.1931 · DOI
- 10.1016/j.celrep.2015.09.042 · DOI
- 10.1111/febs.12842 · DOI
- 10.1111/gtc.12520 · DOI
- 10.1016/j.celrep.2014.09.052 · DOI
- 10.1038/415662a · DOI
- 10.1016/j.cell.2007.05.018 · DOI · Paper page
- 10.1038/17969 · DOI
- 10.1371/journal.pone.0144508 · DOI
- 10.1083/jcb.200309126 · DOI
- 10.1016/j.stem.2015.09.001 · DOI · Paper page
- 10.1038/ncomms5404 · DOI
- 10.1038/ncomms10624 · DOI
- 10.1016/j.cub.2004.03.031 · DOI
- 10.1083/jcb.200309126 · DOI
- Di Cesare, E. et al. Aurora B SUMOylation Is Restricted to Centromeres in Early Mitosis and Requires RANBP2. Cells 12(2023). · DOI
- 10.1242/jcs.197905 · DOI
- 10.1016/j.celrep.2021.110151 · DOI
- 10.1074/jbc.M115.660118 · DOI
- 10.1371/journal.pgen.1009378 · DOI
- 10.1038/s42003-018-0198-0 · DOI
- 10.1091/mbc.E05-09-0893 · DOI
- 10.1186/s12859-021-04344-9 · DOI
- 10.1016/j.cell.2007.07.045 · DOI
- 10.1007/978-1-59745-566-4_11 · DOI
- 10.1016/j.jsb.2005.07.007 · DOI
- 10.1038/nmeth.4193 · DOI
- Zivanov, J. et al. New tools for automated high-resolution cryo-EM structure determination in RELION-3. Elife 7(2018). · DOI
- 10.1107/S0907444910007493 · DOI · Paper page
- 10.1107/S0907444909052925 · DOI · Paper page
- 10.1107/S0907444909042073 · DOI · Paper page
- 10.1002/PRO.3943 · DOI
- 10.1038/nmeth.2019 · DOI · Paper page
- 10.1016/j.ymeth.2016.09.016 · DOI
Metadata
- Authors
- Baytshtok, V., DiMattia, M. A., & Lima, C. D.
- Affiliations
- Memorial Sloan Kettering Cancer Center (US)
- Corresponding author
- Lima, Christopher D.
- Venue
- Nature Communications
- Publisher
- openRxiv
- Published
- 2024-10-05
- Volume
- 16
- Issue
- 1
- Pages
- 6403-6403
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC12246119
- OpenAlex
- W4412181755
- Cited by
- 11
- References
- 1
- Citations by year
- 2026: 9 · 2025: 2
- FWCI
- 3.31
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Howard Hughes Medical Institute
- U.S. Department of Health and Human Services (P30 CA008748, R35 GM118080)
- National Institutes of Health (CA008748)
- National Cancer Institute (CA008748, P30CA008748)
- National Institute of General Medical Sciences (R35GM118080)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Keywords
- RanBP2, Nup358, SUMO1, RanGAP1, Ubc9, CRM1, Ran GTPase, nuclear pore complex, SUMOylation, SUMO E3 ligase, nuclear export signal, mitosis, kinetochore, protein complex structure
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
