CoChat
Sign Up
PreprintCited by 3Cellular transport and secretionExtracellular vesicles in diseaseMicrotubule and mitosis dynamics

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Continuum architecture dynamics of vesicle tethering in exocytosis

Open access
Puig-Tintó, Marta, Ortiz, Sebastian, Meek, Sasha, Coray, Raffaele, Hernández, Altair C., Castellet, Anna, Kramer, Eric, Betancur, Laura I., Hoess, Philipp, Mund, Markus, Izquierdo-Serra, Mercè, Oliva, Baldo, de Marco, Alex, Ries, Jonas, Castaño-Díez, Daniel, Manzo, Carlo, Gallego, Oriol·2025·Cell
DOI 10.1101/2025.02.05.635468

Preprint — published as 10.1016/j.cell.2025.11.038 · Paper page

Open this paper in CoChat — free account

Abstract

SUMMARY: Essential for eukaryotic organisms, multiple copies of the exocyst complex tether each secretory vesicle to the plasma membrane (PM) in constitutive exocytosis. The exocyst higher-order structure (ExHOS) that coordinates the action of these multiple exocysts remains unexplored. We integrated particle-tracking, super-resolution microscopy and cryo-electron tomography in a model that time-resolves the continuum conformational landscape of the ExHOS and functionally annotates its different conformations. We found that 7 exocysts form flexible ring-shaped ExHOS that tether vesicles at <45 nm from the PM. The ExHOS, initially 19 nm in radius, rapidly expands while it pulls the vesicle towards the PM. Subsequently, the ExHOS stabilizes, securing the vesicle at ∼4 nm from the PM. After fusion, Sec18 mediates the ExHOS disassembly when its radius is 38 nm. By resolving the fundamental biophysical principles of tethering we bridged the gap between static isolated structures and the dynamic and multimeric nature of exocytosis.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1101/2025.02.05.635468

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 66

  1. 10.1016/j.ceb.2009.04.007 · DOI · Paper page
  2. 10.1038/nsmb1097 · DOI · Paper page
  3. 10.1083/jcb.201904161 · DOI
  4. 10.1093/emboj/18.4.1071 · DOI
  5. 10.1080/21541248.2016.1213781 · DOI
  6. 10.1021/bi048008z · DOI
  7. 10.1083/jcb.151.2.439 · DOI
  8. 10.1016/0092-8674(93)90376-2 · DOI
  9. 10.1038/nsmb.3146 · DOI
  10. 10.1038/s41594-017-0016-2 · DOI · Paper page
  11. 10.1002/PRO.3863 · DOI
  12. 10.1038/s41467-018-07467-5 · DOI
  13. 10.1016/j.cell.2017.01.004 · DOI
  14. 10.7554/ELIFE.78750 · DOI
  15. 10.7554/elife.04535 · DOI
  16. 10.1083/jcb.202209094 · DOI
  17. 10.1038/s41592-019-0574-9 · DOI
  18. 10.1091/mbc.E22-07-0274 · DOI
  19. 10.7554/eLife.38888 · DOI
  20. 10.1038/nature14148 · DOI
  21. 10.1016/S0092-8674(00)81084-3 · DOI
  22. 10.1038/s41594-024-01388-2 · DOI
  23. 10.1038/s41467-021-25465-y · DOI
  24. 10.1101/2023.07.14.549017 · DOI
  25. 10.1002/1873-3468.14718 · DOI
  26. 10.1016/S0896-6273(00)80664-9 · DOI · Paper page
  27. 10.1091/mbc.E05-10-0917 · DOI
  28. 10.1016/j.str.2019.04.010 · DOI
  29. 10.1038/sj.emboj.7601834 · DOI
  30. 10.1091/mbc.E07-05-0461 · DOI
  31. 10.1083/jcb.201211148 · DOI
  32. 10.1038/nsmb1096 · DOI
  33. 10.1038/s41594-023-01164-8 · DOI · Paper page
  34. 10.1074/jbc.M115.673806 · DOI
  35. 10.1016/j.cub.2022.04.089 · DOI
  36. 10.1016/j.neuron.2014.10.009 · DOI · Paper page
  37. 10.1002/yea.1142 · DOI
  38. 10.1371/journal.pone.0023794 · DOI
  39. 10.1109/83.650848 · DOI
  40. 10.1016/j.jsb.2005.07.007 · DOI
  41. 10.1006/jsbi.1996.0013 · DOI
  42. 10.1038/nmeth.4170 · DOI
  43. 10.1038/s41592-022-01690-1 · DOI
  44. 10.1016/j.jsb.2012.09.006 · DOI · Paper page
  45. 10.1016/j.jsb.2016.07.011 · DOI
  46. 10.1016/J.YJSBX.2022.100068 · DOI
  47. 10.1101/2024.01.05.574336 · DOI
  48. 10.14440/jbm.2014.36 · DOI
  49. 10.1016/J.YMETH.2016.09.016 · DOI
  50. 10.1038/nmeth.2019 · DOI · Paper page
  51. 10.12688/f1000research.27171.1 · DOI
  52. 10.1016/j.molcel.2008.07.020 · DOI
  53. 10.1091/mbc.E08-03-0313 · DOI
  54. 10.1073/pnas.92.11.4947 · DOI · Paper page
  55. 10.15252/embr.202256134 · DOI
  56. 10.1016/j.cell.2018.06.032 · DOI
  57. 10.1364/OE.24.028080 · DOI
  58. 10.1186/s12859-020-03727-8 · DOI
  59. 10.1038/s41592-020-0938-1 · DOI
  60. 10.1109/34.103273 · DOI
  61. 10.1016/J.CSDA.2003.11.008 · DOI
  62. 10.1038/s41586-024-07487-w · DOI · Paper page
  63. 10.1073/pnas.2321447121 · DOI
  64. 10.1093/nar/gkae241 · DOI
  65. 10.1006/jmbi.1993.1626 · DOI · Paper page
  66. 10.1002/PRO.4792 · DOI

Metadata

Authors
Puig-Tintó, M., Ortiz, S., Meek, S., Coray, R., Hernández, A. C., Castellet, A., Kramer, E., Betancur, L. I., Hoess, P., Mund, M., Izquierdo-Serra, M., Oliva, B., de Marco, A., Ries, J., Castaño-Díez, D., Manzo, C., & Gallego, O.
Affiliations
  • Department of Medicine and Life Sciences (MELIS), Universitat Pompeu Fabra (UPF), Barcelona, Spain
  • Universitat Pompeu Fabra (ES)
  • University of the Basque Country (ES)
  • European Molecular Biology Laboratory (DE)
  • Universitat de Barcelona (ES)
  • New York Structural Biology Center (US)
  • University of Vienna (AT)
  • Instituto Biofisika (UPV/EHU, CSIC), University of the Basque Country, Leioa, Spain
  • Universitat de Vic - Universitat Central de Catalunya (ES)
Corresponding author
  • Ries, Jonas
  • Manzo, Carlo
  • Gallego, Oriol
ORCID
Venue
Cell
Publisher
openRxiv
Published
2025-02-05
Volume
189
Issue
4
Pages
1170-1184.e22
ISSN
0092-8674 (ISSN-L)
DOI
10.1101/2025.02.05.635468
OpenAlex
W7124435907
Cited by
3
References
1
Citations by year
2026: 2 · 2025: 1
FWCI
6.76
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion, cc-by)
  • Repositori digital de la UPF (Universitat Pompeu Fabra) (submittedVersion, cc-by)
Funders
  • European Molecular Biology Laboratory
  • Human Frontier Science Program
  • Boehringer Ingelheim Stiftung
  • Agencia Estatal de Investigación (AEI//10.13039/501100011033/, 501100011033, PID2021-127309NB-I00, MICIU/AEI /10.13039/501100011033, 10.13039/501100011033, 13039, .13039/501100011033, 10.13039, 501100011033/FEDER, AEI/10, #CEX2018-000792-M)
  • National Institute of General Medical Sciences (U24GM139171)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Keywords
exocyst complex, vesicle tethering, exocytosis, Sec18, vesicle fusion, plasma membrane, metastable states, secretory vesicles, conformational dynamics, particle tracking, cryo-electron tomography
MeSH terms
Animals, Cell Membrane, Exocytosis, Saccharomyces cerevisiae, Cryoelectron Microscopy, Secretory Vesicles, Saccharomyces cerevisiae Proteins, Vesicular Transport Proteins, Electron Microscope Tomography

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review