Synaptic Vesicle Size and Number Are Regulated by a Clathrin Adaptor Protein Required for Endocytosis
Open accessAbstract
Clathrin-mediated endocytosis is thought to involve the activity of the clathrin adaptor protein AP180. However, the role of this protein in endocytosis in vivo remains unknown. Here, we show that a mutation that eliminates an AP180 homolog (LAP) in Drosophila severely impairs the efficiency of synaptic vesicle endocytosis and alters the normal localization of clathrin in nerve terminals. Most importantly, the size of both synaptic vesicles and quanta is significantly increased in lap mutants. These results provide novel insights into the molecular mechanism of endocytosis and reveal a role for AP180 in regulating vesicle size through a clathrin-dependent reassembly process.
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PICALM has a role in clathrin-mediated endocytosis in non-MG cells.
“and the role of PICALM in clathrin-mediated endocytosis in non-MG cells31,32”
From PICALM Alzheimer’s risk allele causes aberrant lipid droplets in microglia (2025) · cites this paper as [32]
The Results passage on the Drosophila lap gene (page not provided) identifies LAP as an AP180-family protein with a human homolog, and the paper reports that loss of LAP impairs synaptic-vesicle endocytosis. It does not directly establish a role for human PICALM in clathrin-mediated endocytosis in non-MG cells; that part is an extrapolation from the homolog studied. (LLM, conf=0.82)
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Research works printed but not linked: 2
- S.K. (1993). The Drosophila clathrin heavy chain gene: clathrin func- Katz, B. (1969). The release of neural transmitter substances, (Liv- tion is essential in a multicellular organism. Genetics 134, 1119- erpool, UK: Liverpool University Press). 1134.
- Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Clon- ing: A Laboratory Manual, Second Edition. (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press).
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- Authors
- Zhang, B., Koh, Y. H., Beckstead, R. B., Budnik, V., Ganetzky, B., & Bellen, H. J.
- Affiliations
- Howard Hughes Medical Institute (US)
- University of Massachusetts Amherst (US)
- University of Wisconsin–Madison (US)
- Corresponding author
- Bellen, Hugo J
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1998-12
- Volume
- 21
- Issue
- 6
- Pages
- 1465-1475
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2068437038
- Cited by
- 434
- References
- 0
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 9 · 2023: 7 · 2022: 11 · 2021: 17 · 2020: 15 · 2019: 13 · 2018: 15 · 2017: 9 · 2016: 15 · 2015: 17 · 2014: 18 · 2013: 20 · 2012: 17
- FWCI
- 6.75
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Keywords
- clathrin-mediated endocytosis, AP180, clathrin adaptor proteins, synaptic vesicle endocytosis, presynaptic terminals, Drosophila
- MeSH terms
- Amino Acid Sequence, Animals, Axons, DNA-Binding Proteins, Drosophila, Embryo, Nonmammalian, Endocytosis, Molecular Sequence Data, Nerve Endings, Nerve Tissue Proteins, Nuclear Proteins, Phosphoproteins, Synaptic Vesicles, Gene Library, Sequence Alignment, Gene Expression Regulation, Developmental, CCAAT-Enhancer-Binding Proteins, Adaptor Proteins, Vesicular Transport, Monomeric Clathrin Assembly Proteins
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