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PreprintCited by 3Gut microbiota and healthBiochemical and Structural CharacterizationBacterial Genetics and Biotechnology

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Segmented filamentous bacteria undergo a structural transition at their adhesive tip during unicellular to filament development

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Cruz, Ana Raquel, Wimmer, Benedikt, Teo, Teck Hui, Pehau-Arnaudet, Gérard, Winter, Jean-Marie, Gazi, Anastasia, Lafaye, Pierre, Brier, Sébastien, Legrand, Agnès, Dubrovsky-Gaupp, Anna, Bérard, Marion, Aymé, Gabriel, Sartori-Rupp, Anna, Medalia, Ohad, Schnupf, Pamela·2025·Nature Communications
DOI 10.1101/2025.04.09.647586PMC PMC12780176

Preprint — published as 10.1038/s41467-025-66892-5 · Paper page

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Abstract

Segmented filamentous bacteria (SFB) are intestinal commensals that promote immune system development and pathogen protection through intimate attachment to the ileal epithelium. Attachment occurs via the tip of unicellular teardrop-shaped SFB, called intracellular offsprings (IOs), before outgrowth into filaments. To characterize this critical stage of the SFB life-cycle, we imaged SFB using cryo-electron microscopy and tomography. IOs were surrounded by a repetitive surface (S)-layer that became replaced by disordered hair-like structures uniquely at the tip. Upon outgrowth into filaments, the S-layer was exchanged for a morphologically distinct repetitive hair- like layer. The bacterial structures and morphological transition were conserved across SFB from mouse and rat origin, while growth of mouse-SFB under non-attachment conditions in a heterologous host affected the SFB tip length and relative proportion of the tip stages. Moreover, the filament tip displayed surface exposure of the major Th17 antigen, a ubiquitous cell wall protein, underscoring the unique properties of the adhesive tip. This study identifies a novel IO- specific S-layer and reveals a conserved developmental transition of the SFB tip surface including the transient appearance of structures consistent in location and timing with being involved in host cell attachment.

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Research works printed but not linked: 55

  1. 10.1038/s41385-021-00383-w · DOI
  2. 10.1016/j.immuni.2009.08.020 · DOI · Paper page
  3. 10.1016/j.cell.2009.09.033 · DOI · Paper page
  4. 10.1126/science.1254009 · DOI
  5. 10.1016/j.immuni.2014.03.009 · DOI
  6. 10.1084/jem.20230194 · DOI
  7. 10.1038/nature13279 · DOI · Paper page
  8. 10.1128/IAI.00037-15 · DOI · Paper page
  9. 10.1016/j.chom.2024.01.002 · DOI
  10. 10.1016/j.mib.2017.03.004 · DOI
  11. 10.1038/nature14027 · DOI
  12. 10.1111/j.1574-6968.1992.tb04986.x · DOI
  13. 10.1016/j.smim.2013.09.001 · DOI
  14. 10.1016/j.cell.2015.08.058 · DOI · Paper page
  15. 10.1128/AEM.47.2.441-442.1984 · DOI
  16. 10.1128/iai.61.9.4001-4004.1993 · DOI
  17. 10.1128/jb.127.1.572-583.1976 · DOI
  18. Ladinsky, M. S. , Araujo, L. P. , Zhang, X. , Veltri, J. , Galan-Diez, M. , Soualhi, S. , Lee, C. , Irie, K. , Pinker, E. Y. , Narushima, S. , Bandyopadhyay, S. , Nagayama, M. , Elhenawy, W. , Coombes, B. K. , Ferraris, R. P. , Honda, K. , Iliev, I. D. , Gao, N. , Bjorkman, P. J. & Ivanov, I. I. Endocytosis of commensal antigens by intestinal epithelial cells regulates mucosal T cell homeostasis. Science 363, eaat4042 (2019). · DOI
  19. 10.1016/j.chom.2011.08.007 · DOI
  20. 2014, Genome sequence of ‘Candidatus Arthromitus’ sp. strain SFB- mouse-NL, a commensal bacterium with a key role in postnatal maturation of gut immune functions, Genome Announc, 2, e00705
  21. 10.1016/j.chom.2011.08.005 · DOI
  22. 10.1101/gr.131482.111 · DOI
  23. 10.1128/IAI.61.1.303-306.1993 · DOI · Paper page
  24. 10.1007/BF00290989 · DOI
  25. Snel, J. , Heinen, P. P. , Blok, H. J. , Carman, R. J. , Duncan, A. J. , Allen, P. C. & COLLINSh, M. D. Comparison of 16s rRNA Sequences of Segmented Filamentous Bacteria Isolated from Mice, Rats, and Chickens and Proposal of ‘Candidatus Arthromitus’. International Union of Microbiological Societies 45, (1995). · DOI
  26. 10.1111/j.1365-2958.2005.04535.x · DOI
  27. 2017, Structure of the hexagonal surface layer on Caulobacter crescentus cells, Nat Microbiol, 2, 1
  28. 10.1038/s41467-022-28196-w · DOI
  29. 10.7554/eLife.76523 · DOI
  30. 10.1038/nrmicro3213 · DOI
  31. 10.1016/S0966-842X(99)01513-9 · DOI
  32. 2020, Intracellular offspring released from SFB filaments are flagellated, Nat Microbiol, 5, 34
  33. 10.1038/ncb2087 · DOI
  34. Mandelbaum, N. , Zhang, L. , Carasso, S. , Ziv, T. , Lifshiz-Simon, S. , Davidovich, I. , Luz, I. , Berinstein, E. , Gefen, T. , Cooks, T. , Talmon, Y. , Balskus, E. P. & Geva-Zatorsky, N . Extracellular vesicles of the Gram-positive gut symbiont Bifidobacterium longum induce immune-modulatory, anti- inflammatory effects. NPJ Biofilms Microbiomes 9, (2023). · DOI
  35. Brown, L. , Wolf, J. M. , Prados-rosales, R. & Casadevall, A . Through the wall : extracellular vesicles in Gram-positive bacteria , mycobacteria and fungi. Nat Rev Microbiol 13, (2015). · DOI
  36. 10.1016/S0021-9975(97)80036-9 · DOI
  37. 10.1046/j.1365-2958.2002.02852.x · DOI
  38. 10.1038/s41467-023-42826-x · DOI
  39. 10.1038/s41467-020-15650-w · DOI
  40. 10.1111/j.1365-2958.2011.07909.x · DOI
  41. Klein, E. A. , Schlimpert, S. , Hughes, V. , Brun, Y. V. , Thanbichler, M. & Gitai, Z . Physiological role of stalk lengthening in caulobacter crescentus. Commun Integr Biol 6, (2013). · DOI
  42. 10.4161/gmic.19150 · DOI
  43. 10.1038/s41596-020-0320-x · DOI
  44. 10.1017/S1431927603445911 · DOI
  45. 10.1016/j.jsb.2016.06.007 · DOI
  46. 10.1016/j.jsb.2016.07.011 · DOI
  47. 10.1038/s41467-020-18952-1 · DOI
  48. 10.1002/pro.3235 · DOI · Paper page
  49. Schindelin, J. , Arganda-carreras, I. , Frise, E. , Kaynig, V. , Pietzsch, T. , Preibisch, S. , Rueden, C. , Saalfeld, S. , Schmid, B. , Tinevez, J. , White, D. J. , Hartenstein, V. , Tomancak, P. & Cardona, A . Fiji - an Open Source platform for biological image analysis. Nat Methods 9, (2012). · DOI · Paper page
  50. 10.1016/j.molimm.2008.09.008 · DOI
  51. Gransagne, M. , Aymé, G. , Brier, S. , Chauveau-Le Friec, G. , Meriaux, V. , Nowakowski, M. , Dejardin, F. , Levallois, S. , de Melo, G. D. , Donati, F. , Prot, M. , Brûlé, S. , Raynal, B. , Bellalou, J. , Goncalves, P. , Montagutelli, X. , Di Santo, J. P. , Lazarini, F. , England, P. , Petres, S. , Escriou, N. & Lafaye, P . Development of a highly specific and sensitive VHH-based sandwich immunoassay for the detection of the SARS-CoV-2 nucleoprotein. Journal of Biological Chemistry 298, (2022). · DOI
  52. Moutel, S. , El Marjou, A. , Vielemeyer, O. , Nizak, C. , Benaroch, P. , Dübel, S. & Perez, F . A multi-Fc- species system for recombinant antibody production. BMC Biotechnol 9, (2009). · DOI
  53. 10.1038/s41592-019-0459-y · DOI
  54. 10.1093/bioinformatics/btw420 · DOI
  55. 10.1093/NAR/GKAB1038 · DOI · Paper page

Metadata

Authors
Cruz, A. R., Wimmer, B., Teo, T. H., Pehau-Arnaudet, G., Winter, J.-M., Gazi, A., Lafaye, P., Brier, S., Legrand, A., Dubrovsky-Gaupp, A., Bérard, M., Aymé, G., Sartori-Rupp, A., Medalia, O., & Schnupf, P.
Affiliations
  • University of Zurich (CH)
  • Agency for Science, Technology and Research (SG)
  • Institut Pasteur (FR)
  • Centre National de la Recherche Scientifique (FR)
Corresponding author
Schnupf, Pamela
ORCID
Venue
Nature Communications
Publisher
openRxiv
Published
2025-04-09
Volume
17
Issue
1
Pages
222-222
ISSN
2041-1723 (ISSN-L)
DOI
10.1101/2025.04.09.647586
PMC
PMC12780176
OpenAlex
W4417424135
Cited by
3
References
1
Citations by year
2026: 3
FWCI
0.90
Type
journal article
Language
en
Open access
Licence
Locations
  • Nature Communications (publishedVersion, cc-by)
  • bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, other-oa)
  • PubMed (publishedVersion)
  • PubMed Central (publishedVersion, cc-by)
  • HAL (submittedVersion, cc-by)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Zurich Open Repository and Archive (University of Zurich) (submittedVersion, cc-by)
  • Zurich Open Repository and Archive (University of Zurich)
Funders
  • Bill and Melinda Gates Foundation (OPP1141322)
  • Infrastructures en Biologie Santé et Agronomie
  • Equipex (ANR-11-EQPX-0008)
  • Agence Nationale de la Recherche (ANR-10–INSB–04, 10-LABX-62-IBEID, ANR-10–INSB–04-01, 10-INSB-04-01, ANR‐10‐LABX‐62‐IBEID, LABX-62-IBEID, ANR-10, ANR-10-LABX, ANR-10-LABX-0062, ANR-10-INBS-0004, ANR-10-LABX-62, ANR-11-EQPX-0008)
  • Institut National de la Santé et de la Recherche Médicale (ANR-10-INSB-04-01, ANR-10-LABX-62-IBEID)
  • Centre National de la Recherche Scientifique (ANR-10-LABX-62-IBEID, 10-LABX-62-IBEID, ANR-10-LABX-62)
  • Universität Zürich
  • Fondation Bettencourt Schueller (R19214KK)
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
segmented filamentous bacteria, surface layer, host cell attachment, ileal epithelium, bacterial life cycle, B-cell antigens, commensal bacteria, cryo-electron tomography
MeSH terms
Animals, Bacteria, Bacterial Adhesion, Ileum, Intestinal Mucosa, Mice, Inbred C57BL, Cryoelectron Microscopy, Mice, Rats

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