The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
PU.1 induces myeloid lineage commitment in multipotent hematopoietic progenitors
Open accessAbstract
Little is known about the transcription factors that mediate lineage commitment of multipotent hematopoietic precursors. One candidate is the Ets family transcription factor PU.1, which is expressed in myeloid and B cells and is required for the development of both these lineages. We show here that the factor specifically instructs transformed multipotent hematopoietic progenitors to differentiate along the myeloid lineage. This involves not only the up-regulation of myeloid-specific cell surface antigens and the acquisition of myeloid growth-factor dependence but also the down-regulation of progenitor/thrombocyte-specific cell-surface markers and GATA-1. Both effects require an intact PU.1 transactivation domain. Whereas sustained activation of an inducible form of the factor leads to myeloid lineage commitment, short-term activation leads to the formation of immature eosinophils, indicating the existence of a bilineage intermediate. Our results suggest that PU.1 induces myeloid lineage commitment by the suppression of a master regulator of nonmyeloid genes (such as GATA-1) and the concomitant activation of multiple myeloid genes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“PU.1 regulates lineage-specific gene expression in a dose-dependent manner21–23.”
From Lymphoid gene expression supports neuroprotective microglia function (2025) · cites this paper as [21]
Experimental — accuracy not yet measuredIn the citing paper
“All activated macrophages, regardless of polarization state, express high levels of the transcription factors runt-related transcription factor 1 (RUNX1) and PU.1, which are thought to represent global regulators of macrophage phenotypic expression [20–22].”
From Macrophage polarization: an opportunity for improved outcomes in biomaterials and regenerative medicine (2012) · cites this paper as [22]
In the citing paper
“Interestingly, C/EBP-α is also known to cooperate with PU.1 in controlling the expression of the GM-CSF receptor [108].”
From PU.1 and partners: regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis (2009) · cites this paper as [108]
In the citing paper
“During lineage commitment, activation of specific transcription factors has been shown to direct precursor cells to a particular differentiation pathway (3, 41).”
From Negative cross-talk between hematopoietic regulators: GATA proteins repress PU.1 (1999) · cites this paper as [41]
In the citing paper
“and C/EBP⑀ in particular) are essential for the commitment and/or terminal differentiation of myeloid progenitors to the eosinophil lineage (1)(2)(3)(4)(5)(6)(7)(8)(9).”
From Novel Combinatorial Interactions of GATA-1, PU.1, and C/EBPε Isoforms Regulate Transcription of the Gene Encoding Eosinophil Granule Major Basic Protein (2002) · cites this paper as [1]
In the citing paper
“PU.1 probably plays an important role at several stages in the differentiation process, and there is evidence that it is active at an early stage, mediating commitment of multipotential progenitor cells to the myeloid lineage.8”
From Granulocyte inducer C/EBPα inactivates the myeloid master regulator PU.1: possible role in lineage commitment decisions (2002) · cites this paper as [8]
In the citing paper
“PU.1 has been demonstrated to instruct transformed chicken multipotent hematopoietic progenitors to differentiate along the myeloid lineage.8”
From Granulocyte inducer C/EBPα inactivates the myeloid master regulator PU.1: possible role in lineage commitment decisions (2002) · cites this paper as [8]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Kulessa, H., Frampton, J., & Graf, T. (1995) · Genes & Development · doi:10.1101/gad.9.10.1250 · Cited by 418
Self-citationMafB Is an Interaction Partner and Repressor of Ets-1 That Inhibits Erythroid Differentiation
Sieweke, M. H., Tekotte, H., Frampton, J., & Graf, T. (1996) · Cell · doi:10.1016/s0092-8674(00)81081-8 · Cited by 302
Self-citationDistinct C/EBP functions are required for eosinophil lineage commitment and maturation
Nerlov, C., McNagny, K. M., Döderlein, G., Kowenz-Leutz, E., & Graf, T. (1998) · Genes & Development · doi:10.1101/gad.12.15.2413 · Cited by 119
Self-citationActivation of Transcription by PU.1 Requires both Acidic and Glutamine Domains
Klemsz, M. J., & Maki, R. A. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.1.390 · Cited by 93
Research works printed but not linked: 37
- Baron, 1994, Positive regulators of the lineage-specific transcription factor GATA-1 in differentiating erythroid cells., Mol. Cell. Biol., 14, 3108
- 10.1093/infdis/127.1.84 · DOI
- Cairns, 1994, Immortalization of multipotent growth-factor dependent hematopoietic progenitors from mice transgenic for GATA-1 driven SV40 tsA58 gene., EMBO J., 13, 4577, 10.1002/j.1460-2075.1994.tb06779.x · DOI
- Chen, 1995, PU.1 (Spi-1) autoregulates its expression in myeloid cells., Oncogene, 11, 1549
- 10.1016/0003-2697(87)90021-2 · DOI
- 10.1021/bi00276a013 · DOI
- 10.1038/340066a0 · DOI
- Evan, 1985, Isolation of monoclonal antibodies specific for the human c-myc oncogene product., Mol. Cell. Biol., 5, 3610
- Ewert, 1984, Analysis of structural properties and cellular distribution of avian Ia antigen by using monoclonal antibody to monomorphic determinants., J. Immunol., 132, 2524, 10.4049/jimmunol.132.5.2524 · DOI
- Frampton, 1995, v-Myb DNA binding is required to block thrombocytic differentiation of Myb-Ets-transformed multipotent hematopoietic progenitors., EMBO J., 14, 2866, 10.1002/j.1460-2075.1995.tb07286.x · DOI
- 10.1101/gad.10.21.2720 · DOI
- 10.1016/0092-8674(92)90096-U · DOI
- 10.1093/nar/21.22.4998 · DOI
- 10.1073/pnas.91.16.7737 · DOI
- Katz, 1993, The NF-M transcription factor is related to C/EBPβ and plays a role in signal transduction, differentiation and leukemogenesis of avian myelomonocytic cells., EMBO J., 12, 1321, 10.1002/j.1460-2075.1993.tb05777.x · DOI
- 10.1016/0092-8674(90)90219-5 · DOI · Paper page
- 10.1016/0014-4827(83)90065-4 · DOI
- 10.1101/gad.8.1.33 · DOI
- Leutz, 1984, Purification and characterization of cMGF, a novel chicken myelomonocytic growth factor., EMBO J., 3, 3191, 10.1002/j.1460-2075.1984.tb02278.x · DOI
- Leutz, 1989, Molecular cloning of the chicken myelomonocytic growth factor (cMGF) reveals relationship to interleukin 6 and granulocyte colony stimulating factor., EMBO J., 8, 175, 10.1002/j.1460-2075.1989.tb03362.x · DOI
- 10.1038/338039a0 · DOI
- Mandi, 1987, The role of interferon in the adenovirus-induced augmentation of granulocyte-mediated cytotoxicity in chicken., Immunobiology, 174, 210, 10.1016/S0171-2985(87)80040-2 · DOI
- McKercher, 1996, Targeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities., EMBO J., 15, 5647, 10.1002/j.1460-2075.1996.tb00949.x · DOI · Paper page
- McNagny, 1992, Cell surface proteins of chicken hematopoietic progenitors, thrombocytes and eosinophils detected by novel monoclonal antibodies., Leukemia, 6, 975
- McNagny, 1996, The eosinophil-specific cell surface antegen, EOS47, is a chicken homologue of the oncofetal antigen melanotransferrin., Blood, 87, 1343, 10.1182/blood.V87.4.1343.bloodjournal8741343 · DOI
- 10.1083/jcb.138.6.1395 · DOI
- 10.1093/emboj/17.13.3669 · DOI · Paper page
- Ness, 1995, Vintage reds and whites: Combinatorial transcription factor utilization in hematopoietic differentiation., Curr. Opin. Genet. Dev., 4, 718, 10.1016/0959-437X(94)90139-T · DOI
- 10.1073/pnas.92.10.4601 · DOI
- 10.1038/sj.onc.1200807 · DOI · Paper page
- Ray, 1990, The human homologue of the putative proto-oncogene Spi-1: Characterization and expression in tumors., Oncogene, 5, 663
- 10.1016/S0960-9822(02)00610-3 · DOI
- Rossi, 1996, Lineage commitment of transformed haematopoietic progenitors is determined by the level of PKC activity., EMBO J., 15, 1984, 10.1002/j.1460-2075.1996.tb00540.x · DOI
- 10.1126/science.8079170 · DOI · Paper page
- 10.1016/S1074-7613(00)80287-3 · DOI · Paper page
- Tenen, 1997, Transcription factors, normal myeloid development, and leukemia., Blood, 90, 489, 10.1182/blood.V90.2.489 · DOI · Paper page
- 10.1101/gad.4.10.1650 · DOI
Cited by
Sorted by citations
Items 301–399 of 399
Myeloid cells promote interferon signaling-associated deterioration of the hematopoietic system
Feyen, J., Ping, Z., Chen, L., van Dijk, C., van Tienhoven, T. V. D., van Strien, P. M. H., Hoogenboezem, R. M., Wevers, M. J. W., Sanders, M. A., Touw, I. P., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-35318-x · Cited by 10
Dysregulation of NIPBL leads to impaired RUNX1 expression and haematopoietic defects
Mazzola, M., Pezzotta, A., Fazio, G., Rigamonti, A., Bresciani, E., Gaudenzi, G., Pelleri, M. C., Saitta, C., Ferrari, L., Parma, M., et al. (2020) · Journal of Cellular and Molecular Medicine · doi:10.1111/jcmm.15269 · Cited by 10
Mechanisms determining the fate of hematopoietic stem cells.
Lin, S., Zhao, R., Xiao, Y.-R., & Li, P. (2015) · Stem Cell Investigation · doi:10.3978/j.issn.2306-9759.2015.05.01 · Cited by 10
“The current view is that the fate of HSCs is regulated by competition between transcription factor complexes (29).”
BackgroundBalance of apoptotic cell death and survival in allergic diseases
Tian, B., Zhou, H., Xia, L., Shen, H., & Ying, S. (2014) · Microbes and Infection · doi:10.1016/j.micinf.2014.07.004 · Cited by 10
“4) [79,80].”
Pin1‐Mediated Prolyl Isomerization of Runx1 Affects PU.1 Expression in Pre‐Monocytes
Islam, R., Yoon, W., Woo, K. M., Baek, J., & Ryoo, H. (2013) · Journal of Cellular Physiology · doi:10.1002/jcp.24462 · Cited by 10
“5) (Okuda et al., 1996).”
v-Myb can transform and regulate the differentiation of melanocyte precursors
Bell, M., & Frampton, J. (1999) · Oncogene · doi:10.1038/sj.onc.1203161 · Cited by 10
“…haemopoietic cells involving the expression of the protein of interest from a bicistronic retroviral RNA (Frampton et al., 1996; Rossi et al., 1996; Nerlov and Graf, 1998), it may be possible using this system to study the function in melanocytes and their precursors of speci®c transcription…”
BackgroundB-Lymphopoiesis in Fetal Liver, Guided by Chemokines
Kajikhina, K., Tsuneto, M., & Melchers, F. (2016) · Advances in immunology · doi:10.1016/bs.ai.2016.07.002 · Cited by 13
Adult Stem Cel Diferentiation and Trafficking And Their Implications in Disease
Zhuge, Y., Liu, Z., & Velazquez, O. C. (2010) · Advances in experimental medicine and biology · doi:10.1007/978-1-4419-7037-4_12 · Cited by 7
The regulation of chromatin and DNA-methylation patterns in blood cell development.
Bonifer, C., Lefevre, P., & Tagoh, H. (2006) · Current Topics in Microbiology and Immunology · doi:10.1007/3-540-31181-5_1 · Cited by 10
“To quote a few examples: The overexpression of the transcription factor PU.1 in multipotent progenitor cells shifts differentiation towards myeloid cells (DeKoter and Singh 2000; McIvor et al. 2003; Nerlov and Graf 1998; Yamada et al. 2001).”
BackgroundBlood stem cell PU.1 upregulation is a consequence of differentiation without fast autoregulation
Ahmed, N., Etzrodt, M., Dettinger, P., Kull, T., Loeffler, D., Hoppe, P. S., Chavez, J. S., Zhang, Y., Camargo Ortega, G., Hilsenbeck, O., et al. (2022) · Journal of Experimental Medicine · doi:10.1084/jem.20202490 · Cited by 9
LIPA, a risk locus for coronary artery disease: decoding the variant-to-function relationship
Li, F., Flynn, E. D., Ha, P., Zebak, M., Cheng, H.-X., Xue, C.-Y., Shi, J., Wu, X., Wang, Z., Meng, Y., et al. (2025) · European Heart Journal · doi:10.1093/eurheartj/ehaf581 · Cited by 9
King, Z., Desai, S. R., Frank, D. A., & Shastri, A. (2025) · Neoplasia · doi:10.1016/j.neo.2025.101137 · Cited by 11
“PU.1, a master regulator, initiates myeloid differentiation by activating genes necessary for the development of common myeloid progenitors (CMPs) from HSCs [33].”
Role of Neutrophil Extracellular Traps in Hypertension and Their Impact on Target Organs
Yu, F., Chen, J., Zhang, X., Ma, Z., Wang, J., & Wu, Q. (2024) · Journal of Clinical Hypertension · doi:10.1111/jch.14942 · Cited by 9
Xinyi, Y., Vladimirovich, R. I., Beeraka, N. M., Satyavathi, A., Kamble, D., Nikolenko, V. N., Lakshmi, A. N., Basappa, B., Padmanabha Reddy, Y., Fan, R., et al. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-04008-4 · Cited by 11
Tang, Y., Tian, X., Xu, Z., Cai, J., Liu, H., Liu, N., Chen, Z., Chen, S., & Liu, F. (2021) · iScience · doi:10.1016/j.isci.2021.102410 · Cited by 10
Lysophosphatidic Acid and Hematopoiesis: From Microenvironmental Effects to Intracellular Signaling
Lin, K., Chiang, J.-C., Ho, Y.-H., Yao, C.-L., & Lee, H.-Y. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21062015 · Cited by 9
Interplay of transcription factors and microRNAs during embryonic hematopoiesis
Gong, X., Chao, R., Wang, P.-X., Huang, X., Zhang, J., Zhu, X., Zhang, Y., Yang, X., Hou, C.-F., Ji, X., et al. (2017) · Science China Life Sciences · doi:10.1007/s11427-016-0168-0 · Cited by 9
“…and SCL were master regulators involved in both primitive and definitive hematopoiesis (Chen and Zhang, 2001; Gao et al., 2016; Porcher et al., 1996), whereas EKLF (or KLF1) and PU.1 participated in erythropoiesis and lymphogenesis respectively (Lohmann and Bieker, 2008; Nerlov and Graf, 1998).”
BackgroundKang, K., Jung, H., Nam, S., & Lim, J. (2011) · Immune Network · doi:10.4110/in.2011.11.6.348 · Cited by 9
“1, another transcription factor that promotes macrophage and dendritic cell development (3).”
BackgroundThe Importance of Controlling Transcription Elongation at Coding and Noncoding RNA LociPreprint
Scruggs, B. S., & Adelman, K. (2015) · Cold Spring Harbor Symposia on Quantitative Biology · doi:10.1101/sqb.2015.80.027235 · Cited by 9
“PU.1 is particularly appealing candidate for establishing the locations of divergent TSSs in macrophages because it is has properties of a pioneer factor (Nerlov and Graf 1998).”
BackgroundBiological basis of extensive pleiotropy between blood traits and cancer risk
Pardo-Cea, M. Á., Farré, X., Esteve, A., Palade, J., Espín, R., Mateo, F., Alsop, E. B., Alorda, M., Blay, N., Baiges, A., et al. (2024) · Genome Medicine · doi:10.1186/s13073-024-01294-8 · Cited by 12
Lee, A., Park, H., Lim, S.-A., Lim, J.-Y., Koh, J., Jeon, Y., Yang, Y., Lee, M.-S., & Lim, J. (2023) · Journal for ImmunoTherapy of Cancer · doi:10.1136/jitc-2022-005699 · Cited by 8
The Route of Early T Cell Development: Crosstalk between Epigenetic and Transcription Factors
Chiara, V. D., Daxinger, L., & Staal, F. J. T. (2021) · Cells · doi:10.3390/cells10051074 · Cited by 11
Sakai, Y., Rezano, A., Okada, S., Ohtsuki, T., Kawashima, Y., Tsukamoto, T., Suzuki, M., Kohara, M., Takeya, M., Sakaguchi, N., et al. (2020) · Cancers · doi:10.3390/cancers12010204 · Cited by 8
Splitomicin, a SIRT1 Inhibitor, Enhances Hematopoietic Differentiation of Mouse Embryonic Stem Cells
Park, J.-A., Park, S., Park, W.-Y., Han, M.-K., & Lee, Y. (2019) · International Journal of Stem Cells · doi:10.15283/ijsc18040 · Cited by 8
“1, a myeloid specific marker, is an extensively studied hematopoietic transcription factor that is essential for embryonic myeloid development (13).”
BackgroundPU.1, a Shared Transcriptional Regulator of Innate and Adaptive Immune Cell Fates
Singh, H. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.3.1595 · Cited by 7
“Tenen provided strong support for the latter possibility (16, 18, 25, 30).”
BackgroundRodenburg, M., Fischer, M., Engelmann, A., Harbers, S. O., Ziegler, M., Löhler, J., & Stocking, C. (2006) · Journal of Virology · doi:10.1128/jvi.01430-06 · Cited by 6
“cell lineage specificity of the leukemia (17, 30, 35, 53, 55).”
BackgroundPalau, A. M., Segerberg, F., Lidschreiber, M., Lidschreiber, K., Naughton, A., Needhamsen, M., Jung, L. A., Jagodic, M., Cramer, P., Lehmann, S., et al. (2023) · Leukemia · doi:10.1038/s41375-023-01972-3 · Cited by 7
Canonical and atypical chemokine receptors in the neutrophil life cycle
Donato, R. D., Bonecchi, R., & Albano, F. (2023) · Cytokine · doi:10.1016/j.cyto.2023.156297 · Cited by 7
Histamine Receptors and Inflammatory Cells
Ennis, M., Číž, M., Dib, K., Friedman, S., Roopesh, S., Gibbs, B., Levi-Schaffer, F., Lojek, A., Migalovich-Sheikhet, H., O'Mahony, L., et al. (2013) · doi:10.2478/9788376560564.c4 · Cited by 7
“The differentiation of myeloid progenitor cells into mature eosinophils requires critical transcription factors, namely GATA-12, PU.13 and C/EBP4 (McNagny & Graf, 2002; Nerlov & Graf, 1998; Nerlov et al., 1998).”
BackgroundLymphoid Lineage Fate Decision of Hematopoietic Stem Cells
Akashi, K. (2009) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.2009.04570.x · Cited by 7
Mishima, Y., Terui, Y., Mishima, Y., Katsuyama, M., Mori, M., Tomizuka, H., Takizawa, T., Miyazato, A., Ueda, M., Yamada, M., et al. (2002) · Journal of Cellular Physiology · doi:10.1002/jcp.10095 · Cited by 7
Kazerani, M., Cernilogar, F. M., Pasquarella, A., Hinterberger, M., Nuber, A., Klein, L., & Schotta, G. (2024) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.2409656121 · Cited by 6
From Genotype to Phenotype: How Enhancers Control Gene Expression and Cell Identity in Hematopoiesis
Mulet‐Lazaro, R., & Delwel, R. (2023) · HemaSphere · doi:10.1097/hs9.0000000000000969 · Cited by 11
Franza, M., Albanesi, J., Mancini, B., Pennisi, R., Leone, S., Acconcia, F., Bianchi, F., & di Masi, A. (2023) · Biochemical Pharmacology · doi:10.1016/j.bcp.2023.115675 · Cited by 7
Wurm, M., Kowalski, J. P., Heckl, D., Zhang, X.-B., Nelson, V., Beard, B. C., & Kiem, H. (2013) · Experimental Hematology · doi:10.1016/j.exphem.2013.10.004 · Cited by 8
Involvement of PU.1 in mouse adar-1 gene transcription induced by high-dose esiRNA.
Wu, T., Zhao, Y.-C., Hao, Z.-J., Zhao, H.-F., & Wang, W.-R. (2009) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2009.04.021 · Cited by 6
Kim, K., Choi, Y. H., Kim, H.-H., & Cheong, J. (2009) · Experimental and Molecular Medicine · doi:10.3858/emm.2009.41.6.048 · Cited by 6
Anzai, H., Ikawa, Y., & Atsumi, T. (2001) · Biochemical and Biophysical Research Communications - BBRC · doi:10.1006/bbrc.2001.4674 · Cited by 6
Brain Cancer-Activated Microglia: A Potential Role for Sphingolipids
Bottai, D., Adami, R., Paroni, R. C., & Ghidoni, R. (2019) · Current Medicinal Chemistry · doi:10.2174/0929867326666190506120213 · Cited by 6
Guo, C., Griffin, M., Morgan, A. G., Foster, D. S., Parker, J. B. L., Januszyk, M., Lindsay, H. G., Guardino, N. J., Reveron‐Thornton, R. F., Xie, P. Y., et al. (2026) · Cell Reports · doi:10.1016/j.celrep.2025.116849 · Cited by 5
Herrera, J., Bensussen, A., García‐Gómez, M. L., Garay‐Arroyo, A., & Alvarez-Buylla, E. R. (2024) · npj Systems Biology and Applications · doi:10.1038/s41540-024-00469-8 · Cited by 5
Guglielmi, V., Lam, D., & D'Angelo, M. A. (2024) · Science Advances · doi:10.1126/sciadv.adn8963 · Cited by 5
How Azanucleosides Affect Myeloid Cell Fate
Stein, A., Platzbecker, U., & Cross, M. A. (2022) · Cells · doi:10.3390/cells11162589 · Cited by 7
“As commitment takes place, one lineage programme crosses an activation threshold and is fixed in such a way that suppresses competing lineage programmes [38] while linking subsequent proliferation with progressive differentiation [39].”
BackgroundLaffey, K. G., Du, J., Schrum, A. G., & Ackerman, S. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms221910245 · Cited by 5
Marycz, K., Turlej, E., Kornicka-Garbowska, K., Zachanowicz, E., Tomaszewska, A., Kulpa-Greszta, M., & Pązik, R. (2021) · Materials · doi:10.3390/ma14175010 · Cited by 5
Stochastic multistationarity in a model of the hematopoietic stem cell differentiation network
Al-Radhawi, M. A., Kumar, N. S., Sontag, E. D., & Vecchio, D. D. (2018) · IEEE Conference on Decision and Control (CDC) · doi:10.1109/cdc.2018.8619300 · Cited by 6
“1GATA-1 GRN, which is involved in lineage determination in hematopoietic stem cells (HSCs) [8], [9].”
Transcriptional Regulation of Hematopoietic Stem Cells
Göttgens, B., & Green, A. R. (2004) · Elsevier eBooks · doi:10.1016/b978-012436643-5/50119-x · Cited by 5
Woo, S.-J., Songodan, T., & Han, J. Y. (2026) · Frontiers in Immunology · doi:10.3389/fimmu.2026.1755331 · Cited by 4
“Low to moderate PU.1 level permits B cell development in concert with IRF8, while high PU.1 expression induces myeloid lineage commitment (34, 35).”
Barabino, S. M. L., Citterio, E., & Ronchi, A. E. (2021) · Cancers · doi:10.3390/cancers13153753 · Cited by 4
“1 is reciprocal: PU1 blocks GATA1 transcription [115] and protein [116] whereas GATA1, as well as GATA2, inhibit PU.”
BackgroundHaematopoietic stem cell reprogramming and the hope for a universal blood product
Durán, J. G. B., Lis, R., & Rafii, S. (2019) · FEBS Letters · doi:10.1002/1873-3468.13681 · Cited by 4
Bokes, P., & King, J. R. (2018) · Mathematical Medicine and Biology A Journal of the IMA · doi:10.1093/imammb/dqy003 · Cited by 4
“A commitment decision can be reversed by a transient forced expression of the transcription factor associated with the alternative genetic programme (Kulessa et al., 1995; Nerlov and Graf, 1998).”
BackgroundTraver, D. J., & Akashi, K. (2004) · Elsevier eBooks · doi:10.1016/b978-012436643-5/50124-3 · Cited by 2
Overview of Immunology in the Mouse: Molecular and Cellular Immunology
Quimby, F., & Chaplin, D. (2007) · Elsevier eBooks · doi:10.1016/b978-012369454-6/50078-9 · Cited by 4
Li, J., Fu, L., Li, Y., Sun, W., Yao, Y., Jia, W., Li, H., Liu, H., Guo, P., Wang, Y., et al. (2024) · Scientific Data · doi:10.1038/s41597-024-03558-9 · Cited by 4
Yashar, W. M., Curtiss, B. M., Coleman, D. J., Van-Campen, J., Kong, G. L., Macaraeg, J., Estabrook, J., Demir, E., Long, N., Bottomly, D., et al. (2022) · bioRxiv · doi:10.1101/2022.01.17.476469 · Cited by 3
Squillace, D., Checkel, J., Tefferi, A., Kita, H., & Gleich, G. (2021) · JIM - Journal of Immunological Methods · doi:10.1016/j.jim.2021.113015 · Cited by 3
To bi or not to bi: Acute erythroid leukemias and hematopoietic lineage choice
Genua, C. D., & Nerlov, C. (2021) · Experimental Hematology · doi:10.1016/j.exphem.2021.02.006 · Cited by 3
Self-citationEncounters across networks: Windows into principles of genomic regulation
Rothenberg, E. V. (2019) · Marine Genomics · doi:10.1016/j.margen.2019.01.003 · Cited by 4
Asymmetric Regulation of Peripheral Genes by Two Transcriptional Regulatory Networks
Li, J.-R., Suzuki, T., Nishimura, H., Kishima, M., Maeda, S., & Suzuki, H. (2016) · PLoS ONE · doi:10.1371/journal.pone.0160459 · Cited by 3
Animals have expanded the evolutionary legacy of unicellular ancestors in blood cells
Nagahata, Y., Nishimura, Y., Kaitani, R., Leong, J. C. K., Oda‐Ishii, I., Kohtsuka, H., Abe, S., Ishida, T., Carmona-Rivas, M., Najle, S. R., et al. (2026) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2528110123 · Cited by 3
Neuroinflammation in Ischemic Stroke: Mechanisms, Systemic Interactions, and Therapeutic Targets
Soares, E., Westendorp, W. F., Coutinho, J. M., Brouwer, M. C., & van de Beek, D. (2026) · Translational Stroke Research · doi:10.1007/s12975-026-01442-9 · Cited by 2
“This expansion corresponds to the upregulation of the transcription factor PU.1 [72], known for inducing myeloid lineage commitment in hematopoietic progenitors and myeloid cells [73].”
Zfp263 is a transcriptional checkpoint of neutrophil developmentPreprint
Devaprasad, A., Ai, Z., Mukherjee, A. K., Pánczél, Á., Khoyratty, T. E., Ballesteros, I., Rubio-Ponce, A., van Grinsven, E., Wang, L., Salafranca, J., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.08.22.609132 · Cited by 5
“Pu.1 and C/ebp α, were shown to guide neutrophil lineage commitment and early differentiation (24, 25); whereas C/ebp ɛ and Gfi1 promoted neutrophil terminal differentiation (9, 26).”
BackgroundRegulation of Microglia for the Treatment of Glioma
Zhou, S., & Cisse, B. (2021) · World Neurosurgery · doi:10.1016/j.wneu.2021.06.107 · Cited by 2
Reprogramming cooperative monotone dynamical systems
Shah, R., & Vecchio, D. D. (2018) · IEEE Conference on Decision and Control · doi:10.1109/cdc.2018.8618649 · Cited by 2
“It was only in recent years that ground-breaking experiments demonstrated that the process can actually be reversed [4], although with very low efficiency [5], and that cell types can also be interconverted [6], that is, the fate of a cell can be reprogrammed [7].”
BackgroundYuan, R., Ao, P., Wang, G., & Su, H. (2017) · Scientia Sinica Vitae · doi:10.1360/n052016-00309 · Cited by 2
Epigenetic regulation of cellular and cytomegalovirus genes during myeloid cell development
Liu, X. (2017) · Internal Medicine Review · doi:10.18103/imr.v3i3.385 · Cited by 2
“1 protein, encoded by Spi1 gene, is required for generation of common myeloid progenitors (CMPs) from hematopoietic stem cells (HSCs) and formation of the myeloid transcription network [18, 19].”
BackgroundCory, S. (2015) · The Journal of Immunology · doi:10.4049/jimmunol.1501277 · Cited by 3
“1/Spi1 expression provoked the reverse (7, 8).”
The Maintaining and Directed Differentiation of Hematopoietic Stem Cells Under Microgravity
Wang, P., Qian, J., Tian, H., & Zhao, Y. X. (2019) · Research for development · doi:10.1007/978-981-13-6325-2_9 · Cited by 4
The Road to Commitment: Lineage Restriction Events in Hematopoiesis
Månsson, R., Zandi, S., Bryder, D., & Sigvardsson, M. (2009) · Springer eBooks · doi:10.1007/978-0-387-85816-6_2 · Cited by 2
DNA Sequence-Specific Transcription Factors
Auron, P. E. (2005) · Elsevier eBooks · doi:10.1016/b978-012455900-4/50269-5 · Cited by 2
Regulatory Co-options in the Evolution of Deuterostome Immune Systems
Rothenberg, E. V., & Davidson, E. H. (2003) · Humana Press eBooks · doi:10.1007/978-1-59259-320-0_4 · Cited by 5
Shibata, S., Chonabayashi, K., Nakamura, H., Matsumura, Y., Kawahara, H., Okamoto, Y., Yamamoto, S., Nishikawa, M., Ochi, Y., Uchiyama, Y., et al. (2026) · Leukemia · doi:10.1038/s41375-026-03061-7 · Cited by 1
Sheen, K., Song, C., Kwon, K., Kim, S., Park, H.-J., & Kim, M. S. (2026) · Oral Diseases · doi:10.1111/odi.70332 · Cited by 1
Mechanisms coordinating exit from the stem cell state in mammalsPreprint
Park, E. J., Levin-Ferreyra, F., & Stefano, B. D. (2026) · Genes & Development · doi:10.1101/gad.353584.125 · Cited by 1
“…2014; Wang et al. 2025; Wu et al. 2025; Kong et al. 2026), while the TFs PU.1 and GATA2 regulate both stem cell function and the identity of differentiated cells in the hematopoietic system (Scott et al. 1994; Tsai et al. 1994; Nerlov and Graf 1998; Rodrigues et al. 2005, 2008; Staber et al. 2013).”
Kim, D., Park, S., Kwon, Y.-R., Yoon, H., Cho, B., Baek, I., Kang, J. H., Park, T.-E., Corces, M., Kim, H.-J., et al. (2025) · iScience · doi:10.1016/j.isci.2025.113297 · Cited by 1
“For the MDS-NR peaks, we observed a significant enrichment of PU.1 family TF-binding motifs, SPI1/SPIB, which serve as master regulators of myeloid lineage commitment 23,24 and lymphoid suppression.”
Yu, R.-J., Kang, S.-M., & Kim, H. P. (2015) · doi:10.5352/jls.2015.25.7.810 · Cited by 1
“Their expression is limited to specific tissues and cells; for example, GATA-1 is expressed in red blood cells, megakaryocytes, and mast cells [16, 32, 35].”
BackgroundThe Road to Pathogenesis: Charting the Development of LSCs and Pre-LSCs
Levine, R. (2019) · Frontiers Research Topics · doi:10.3389/978-2-88945-767-0 · Cited by 1
Choe, Y., & Lee, Y. C. (2008) · Tuberculosis & respiratory diseases · doi:10.4046/trd.2008.64.1.1 · Cited by 1
Single-Cell Gene-Expression Analysis by Quantitative RT-PCR
Warren, L. A. (2008) · Polymer Journal · doi:10.7907/pj42-xb85
Gao, J., Li, Y., Zhao, D., Guan, X., Motsinger, K., Gutierrez, A., Lamichhane, A., Luo, Y., Scott-Browne, J. P., Wei, C. H., et al. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.113699
A Retrospective Review of 1821 Cases of Eosinophilia in a Single‐Center Study in Eastern China
Ma, Y.-N., Wang, L.-L., Cheng, Y.-X., Yang, X.-F., & Chen, S.-N. (2026) · eJHaem · doi:10.1002/jha2.70369
Ramani, M., Nguyen, N., Solis, J., Tenen, D. G., & Trinh, B. Q. (2026) · Biology · doi:10.3390/biology15181657
“High PU.1 expression promotes macrophage differentiation in fetal liver progenitors, bone marrow precursors, and multipotent cell models [10,100,111].”
Woo, S. J., Songodan, T., Seo, M., & Han, J. Y. (2026) · Frontiers in Immunology · doi:10.3389/fimmu.2026.1931366 · Cited by 1
“For instance, the PU.1 is expressed in chicken myeloid cells, binds the macrophage CSF1R promoter, and drives the myelomonocytic differentiation pathway (32), although it remains to be explored whether low expression of PU.1 supports heterophil development as shown in mammals.”
Wang, Z.-Q., & Zhou, D.-B. (2026) · Translational Cancer Research · doi:10.21037/tcr-2025-aw-2307
“In addition, suppression or downregulation of differentiation-associated genes, such as the key myeloid transcription factor PU.1, may also induce the reversion of differentiated AML cells to a less mature, leukemogenic state (11,12).”
PU.1 inhibition sensitizes stem-monocytic AML to BCL2 blockadePreprint
Yashar, W. M., Pacentine, I. V., Taherinasab, A., Nguyen, T., Worme, S. J., Tsuchiya, M., Lusardi, T. A., Hutchinson, C., Fillmore, T., Posso, C., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.01.20.700677
“Pharmacologic inhibition of PU.1 enhanced venetoclax efficacy in stem-monocytic AML cell line models and in primary patient samples, revealing a tractable therapeutic vulnerability in this population 14,15 .”
Rora Regulates Hematopoietic Stem Cell Phenotypes and Progression of Chronic Myelogenous Leukemia.
Li, N., Feng, Y.-L., Wang, N., He, W., Li, H., Cui, X., Wang, B.-C., Qin, R., Qiu, H.-D., Qiu, Q., et al. (2026) · American Journal of Pathology · doi:10.1016/j.ajpath.2025.12.010
Regulation of an lncRNA irf8 by the Ikzf1/Myb complex drives neutrophil development
He, Y., Li, Y., Shao, Y.-B., Huang, X., He, J., Liu, S., Xiao, Y., Li, Z., Hou, Y., Luo, L., et al. (2025) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.2502741122
Antagonistic effects of PU.1 on Gfi-1B-induced erythroid colony formation in human cord blood cells
Manabe, N., Sakurai, T., Kihara‐Negishi, F., Nakazawa, Y., Yamada, T., Iwama, A., & Oikawa, T. (2025) · Cellular and Molecular Biology · doi:10.14715/cmb/2025.71.3.6
“PU.1 is up-regulated during differentiation into the myeloid lineage but is down-regu - lated during differentiation into the erythroid lineage, and Gfi-1B exhibits the opposite behavior [10, 17, 20].”
Ober-Reynolds, B., Wang, C., Ko, J., Rios, E. J., Aasi, S., Davis, M. M., Oro, A., & Greenleaf, W. (2022) · bioRxiv · doi:10.1101/2022.09.28.509620
Lipopolysaccharide-dependent transcriptional regulation of PU.1 in microglial cells
Mandal, C., Yoon, T., Park, J. Y., Jung, K. H., & Chai, Y. G. (2019) · Molecular & Cellular Toxicology · doi:10.1007/s13273-019-00057-6
Rôle de Tif1gamma dans les différenciations granulo-monocytaire et macrophagique
Chrétien, M. (2015) · theses.fr (ABES) · doi:10.70675/615e6a36z0efaz447ez8579zb24a90ad1e76
Epigenetic reprogramming and DNA demethylation
Bagci, H. (2014) · Spiral (Imperial College London) · doi:10.25560/45352
“1 in erythroid-megakaryocytic cells promotes their switch to monocytes by repressing GATA1 (Nerlov and Graf, 1998).”
BackgroundTHE MOLECULAR, CELLULAR, AND GENETIC BASIS OF HEMOGLOBIN DISORDERS
Steinberg, M. H., Forget, B. G., Higgs, D. R., & Weatherall, D. J. (2009) · Cambridge University Press eBooks · doi:10.1017/cbo9780511596582.004 · Cited by 1
Rao, L. G., & Murray, T. M. (2000) · Drug Development Research · doi:10.1002/(sici)1098-2299(200003)49:3 · Cited by 10
Malaviya, R., Laskin, J. D., & Laskin, D. L. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-801238-3.64249-6 · Cited by 1
The Macrophage Epigenome and the Control of Inflammatory Gene Expression
Polletti, S., Curina, A., Natoli, G., & Ghisletti, S. (2014) · Epigenetics and human health · doi:10.1007/978-3-642-45198-0_15
Cellular Reprogramming and Fate Conversion
Ieda, M. (2012) · Humana Press eBooks · doi:10.1007/978-1-61779-940-2_12
“defined transcription factors [7–13].”
Functional analysis of the homeobox gene HOXB4 in primitive hematopoietic cells
Antonchuk, J. (2002) · doi:10.14288/1.0099658
“l in the same line led to myeloid differentiation (Nerlov and Graf, 1998).”
Background
Metadata
- Authors
- Nerlov, C., & Graf, T.
- Affiliations
- European Molecular Biology Laboratory (DE)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1998-08-01
- Volume
- 12
- Issue
- 15
- Pages
- 2403-2412
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC317050
- OpenAlex
- W1974612283
- Cited by
- 449
- References
- 46
- Citations by year
- 2026: 13 · 2025: 9 · 2024: 12 · 2023: 16 · 2022: 7 · 2021: 22 · 2020: 18 · 2019: 19 · 2018: 13 · 2017: 21 · 2016: 16 · 2015: 17 · 2014: 15 · 2013: 27 · 2012: 17
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Sundhed og Sygdom, Det Frie Forskningsråd
- Medical Research Council
- Topics
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
- Keywords
- PU.1, ETS transcription factors, myeloid lineage commitment, hematopoietic progenitor cells, GATA-1, transactivation domain, eosinophils, cell surface markers
- MeSH terms
- Proto-Oncogene Protein Spi-1, Alleles, Animals, Cell Differentiation, Chick Embryo, DNA-Binding Proteins, Hematopoiesis, Hematopoietic Stem Cells, Humans, Proto-Oncogene Proteins, Transcription Factors, Transformation, Genetic, Trans-Activators, Gene Expression Regulation, Developmental, Erythroid-Specific DNA-Binding Factors, GATA1 Transcription Factor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
