The DOI matches its Crossref record; 13 of the 13 references it cites were found in a registry or an index.
The order of expression of transcription factors directs hierarchical specification of hematopoietic lineages
Abstract
The mechanism of lineage specification in multipotent stem cells has not been fully understood. We recently isolated progenitors with the eosinophil, basophil, or mast cell lineage potential, all of which originate from granulocyte/monocyte progenitors (GMPs). By using these prospectively purified progenitors, we show here that the expression timing of GATA-2 and CCAAT enhancer-binding protein α (C/EBPα) can differentially control their lineage commitment. The expression of GATA-2 instructed C/EBPα-expressing GMPs to commit exclusively into the eosinophil lineage, while it induced basophil and/or mast cell lineage commitment if C/EBPα was suppressed at the GMP stage. Furthermore, simply by switching the order of C/EBPα and GATA-2 transduction, even lymphoid-committed progenitors recaptured these developmental processes to be reprogrammed into each of these lineages. We propose that the order of expression of key transcription factors is critical for their interplay to selectively drive lineage specification programs, by which stem cells could generate multiple lineage cells in a hierarchical manner.
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“Reports on the role of GATA-2 in eosinophil differentiation and function have shown that it can have positive, as well as negative regulatory functions. 8 -14”
From RhoH is a negative regulator of eosinophilopoiesis (2016) · cites this paper as [11]
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Cites
A clonogenic common myeloid progenitor that gives rise to all myeloid lineages
Akashi, K., Traver, D., Miyamoto, T., & Weissman, I. L. (2000) · Nature · doi:10.1038/35004599 · Cited by 2,617
Self-citationZhang, D.-E., Zhang, P., Wang, N.-D., Hetherington, C. J., Darlington, G. J., & Tenen, D. G. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.2.569 · Cited by 910
Self-citationRegulation of B lymphocyte and macrophage development by graded expression of PU.1
DeKoter, R. P., & Singh, H. (2000) · Science · doi:10.1126/science.288.5470.1439 · Cited by 749
Negative cross-talk between hematopoietic regulators: GATA proteins repress PU.1
Zhang, P., Behre, G., Pan, J., Iwama, A., Wara-aswapati, N., Radomska, H. S., Auron, P. E., Tenen, D. G., & Sun, Z. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.15.8705 · Cited by 459
Self-citationRekhtman, N., Radparvar, F., Evans, T., & Skoultchi, A. I. (1999) · Genes & Development · doi:10.1101/gad.13.11.1398 · Cited by 454 · cited 1×
“Eosinophils, basophils, and mast cells are multifunctional hematopoietic effectors that cooperate to mount a variety of allergic and innate immune responses (Rothenberg 1998; Galli 2000; Wedemeyer et al. 2000).”
BackgroundPU.1 induces myeloid lineage commitment in multipotent hematopoietic progenitors
Nerlov, C., & Graf, T. (1998) · Genes & Development · doi:10.1101/gad.12.15.2403 · Cited by 449 · cited 2×
“2000), and vice versa (Nerlov and Graf 1998; Rekhtman et al. 1999; Zhang et al. 1999, 2000; Walsh et al. 2002), suggesting that these antagonistic effects may be critical at least for the MegE versus the granulocyte/monocyte (GM) lineage commitment.”
BackgroundKulessa, H., Frampton, J., & Graf, T. (1995) · Genes & Development · doi:10.1101/gad.9.10.1250 · Cited by 418
Dahl, R., Walsh, J. C., Lancki, D., Laslo, P., Iyer, S. R., Singh, H., & Simon, M. C. (2003) · Nature Immunology · doi:10.1038/ni973 · Cited by 380
PU.1 inhibits GATA-1 function and erythroid differentiation by blocking GATA-1 DNA binding
Zhang, P., Zhang, X., Iwama, A., Yu, C., Smith, K. A., Mueller, B. U., Narravula, S., Torbett, B. E., Orkin, S. H., & Tenen, D. G. (2000) · Blood · doi:10.1182/blood.v96.8.2641 · Cited by 368
Self-citationNerlov, C., Querfurth, E., Kulessa, H., & Graf, T. (2000) · Blood · doi:10.1182/blood.v95.8.2543 · Cited by 366 · cited 1×
“It has been proposed that antagonistic or cooperative effects of multiple transcription factors, called a “transcription factor network” (Sieweke and Graf 1998; Orkin 2000), play a pivotal role in establishment of lineage diversities from stem cells.”
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Research works printed but not linked: 40
- 10.1073/pnas.0509148102 · DOI · Paper page
- 10.1016/j.immuni.2005.10.009 · DOI
- 10.1084/jem.182.1.49 · DOI
- 10.1126/science.1068999 · DOI
- 10.1101/gad.862301 · DOI
- 10.1073/pnas.93.22.12355 · DOI · Paper page
- 10.1097/00062752-200001000-00007 · DOI
- 10.1046/j.1365-2443.1998.00166.x · DOI
- 10.1093/emboj/cdf368 · DOI
- 10.1084/jem.20020170 · DOI · Paper page
- 10.1016/S1074-7613(03)00242-5 · DOI
- 10.1084/jem.20050548 · DOI
- 10.1084/jem.20021843 · DOI
- Jippo,, 1996, Abundant expression of transcription factor GATA-2 in proliferating but not in differentiated mast cells in tissues of mice: Demonstration by in situ hybridization, Blood, 87, 993, 10.1182/blood.V87.3.993.bloodjournal873993 · DOI
- 10.1016/S0092-8674(00)80453-5 · DOI · Paper page
- 10.1038/35030112 · DOI
- 10.1073/pnas.172384399 · DOI
- 10.1038/344444a0 · DOI
- 10.1084/jem.20020636 · DOI
- 10.1084/jem.20021149 · DOI
- 10.1038/nrc1363 · DOI
- Orkin,, 2000, Diversification of haematopoietic stem cells to specific lineages, Nat. Rev. Genet., 1, 57, 10.1038/35049577 · DOI
- 10.1101/gad.177200 · DOI
- 10.1056/NEJM199805283382206 · DOI
- 10.1126/science.8079170 · DOI · Paper page
- 10.1016/S0959-437X(98)80009-9 · DOI · Paper page
- Takahashi,, 2000, GATA factor transgenes under GATA-1 locus control rescue germline GATA-1 mutant deficiencies, Blood, 96, 910, 10.1182/blood.V96.3.910 · DOI
- Tenen,, 1997, Transcription factors, normal myeloid development, and leukemia, Blood, 90, 489, 10.1182/blood.V90.2.489 · DOI · Paper page
- 10.1126/science.290.5499.2152 · DOI
- Tsai,, 1997, Transcription factor GATA-2 is required for proliferation/survival of early hematopoietic cells and mast cell formation, but not for erythroid and myeloid terminal differentiation, Blood, 89, 3636, 10.1182/blood.V89.10.3636 · DOI
- 10.1006/dbio.1997.8842 · DOI
- 10.1016/S0092-8674(00)80318-9 · DOI · Paper page
- 10.1101/gad.12.8.1176 · DOI
- 10.1016/S0952-7915(00)00154-0 · DOI
- Weil,, 1987, A hybrid eosinophilic–basophilic granulocyte in chronic granulocytic leukemia, Am. J. Clin. Pathol., 87, 66, 10.1093/ajcp/87.1.66 · DOI
- 10.1016/S0092-8674(04)00419-2 · DOI
- 10.1093/intimm/2.2.181 · DOI
- 10.1093/nar/gnh125 · DOI
- 10.1016/j.immuni.2004.11.006 · DOI
- Zon,, 1993, Expression of mRNA for the GATA-binding proteins in human eosinophils and basophils: Potential role in gene transcription, Blood, 81, 3234, 10.1182/blood.V81.12.3234.3234 · DOI
Cited by
Forcing cells to change lineages
Graf, T., & Enver, T. (2009) · Nature · doi:10.1038/nature08533 · Cited by 899
Pronk, C. J. H., Rossi, D. J., Månsson, R., Attema, J. L., Norddahl, G. L., Chan, C. K. F., Sigvardsson, M., Weissman, I. L., & Bryder, D. (2007) · Cell Stem Cell · doi:10.1016/j.stem.2007.07.005 · Cited by 671
Myeloid Lineage Commitment from the Hematopoietic Stem Cell
Iwasaki, H., & Akashi, K. (2007) · Immunity · doi:10.1016/j.immuni.2007.06.004 · Cited by 394
Self-citationReprogramming Committed Murine Blood Cells to Induced Hematopoietic Stem Cells with Defined Factors
Riddell, J., Gazit, R., Garrison, B. S., Guo, G., Saadatpour, A., Mandal, P. K., Ebina, W., Volchkov, P., Yuan, G.-C., Orkin, S. H., et al. (2014) · Cell · doi:10.1016/j.cell.2014.04.006 · Cited by 323
Arinobu, Y., Mizuno, S.-I., Chong, Y., Shigematsu, H., Iino, T., Iwasaki, H., Graf, T., Mayfield, R., Chan, S., Kastner, P., et al. (2007) · Cell Stem Cell · doi:10.1016/j.stem.2007.07.004 · Cited by 309
Self-citation
Metadata
- Authors
- Iwasaki, H., Mizuno, S.-I., Arinobu, Y., Ozawa, H., Mori, Y., Shigematsu, H., Takatsu, K., Tenen, D. G., & Akashi, K.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2006-11-01
- Volume
- 20
- Issue
- 21
- Pages
- 3010-3021
- ISSN
- 0890-9369
- 1549-5477
- Cited by
- 281
- References
- 13
- Language
- en
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