The p120 catenin partner Kaiso is a DNA methylation-dependent transcriptional repressor
Open accessAbstract
We describe a novel mammalian DNA binding activity that requires at least two symmetrically methylated CpG dinucleotides in its recognition sequence, preferably within the sequence 5′CGCG. A key component of the activity is Kaiso, a protein with POZ and zinc-finger domains that is known to associate with p120 catenin. We find that Kaiso behaves as a methylation-dependent transcriptional repressor in transient transfection assays. Kaiso is a constituent of one of two methyl–CpG binding complexes originally designated as MeCP1. The data suggest that zinc-finger motifs are responsible for DNA binding, and may therefore target repression to specific methylated regions of the genome. As Kaiso associates with p120 catenin, Kaiso may link events at the cell surface with DNA methylation-dependent gene silencing.
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Kaiso binds a specific methylated sequence through its C2H2 zinc-finger domains.
“the transcriptional regulator Kaiso, which contains POZ (pox virus and zinc-finger) and zinc-finger domains, was found to bind to a specific methylated sequence with its C2H2 zinc-finger domains41”
From Transcription factors as readers and effectors of DNA methylation (2016) · cites this paper as [41]
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Cites
1 retracted
Transient depletion of xDnmt1 leads to premature gene activation in Xenopus embryosPreprint
Stancheva, I., & Meehan, R. R. (2000) · Genes & Development · doi:10.1101/gad.14.3.313 · Cited by 184 · see citing works
RetractedDignam, J. D., Lebovitz, R. M., & Roeder, R. G. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.5.1475 · Cited by 11,262 · see citing works
Nan, X., Ng, H.-H., Johnson, C. A., Laherty, C. D., Turner, B. M., Eisenman, R. N., & Bird, A. (1998) · Nature · doi:10.1038/30764 · Cited by 3,440 · see citing works
Self-citationMethylated DNA and MeCP2 recruit histone deacetylase to repress transcription
Jones, P. L., Veenstra, G. J. C., Wade, P. A., Vermaak, D., Kass, S. U., Landsberger, N., Strouboulis, J., & Wolffe, A. P. (1998) · Nature Genetics · doi:10.1038/561 · Cited by 2,734 · see citing works
A mouse Mecp2-null mutation causes neurological symptoms that mimic Rett syndrome
Guy, J., Hendrich, B., Holmes, M., Martin, J. E., & Bird, A. (2001) · Nature Genetics · doi:10.1038/85899 · Cited by 1,558 · see citing works
Self-citationIdentification and Characterization of a Family of Mammalian Methyl-CpG Binding Proteins
Hendrich, B., & Bird, A. P. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.11.6538 · Cited by 1,352 · see citing works
Self-citationMBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex
Ng, H., Zhang, Y., Hendrich, B., Johnson, C. A., Turner, B. M., Erdjument‐Bromage, H., Tempst, P., Reinberg, D., & Bird, A. P. (1999) · Nature Genetics · doi:10.1038/12659 · Cited by 936 · see citing works
Self-citationInactivation of the E-Cadherin-Mediated Cell Adhesion System in Human Cancers
Hirohashi, S. (1998) · American Journal Of Pathology · doi:10.1016/s0002-9440(10)65575-7 · Cited by 839 · see citing works
Mi-2 complex couples DNA methylation to chromatin remodelling and histone deacetylation
Wade, P. A., Gégonne, A., Jones, P. L., Ballestar, E., Aubry, F., & Wolffe, A. P. (1999) · Nature Genetics · doi:10.1038/12664 · Cited by 811 · see citing works
DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein
Boyes, J., & Bird, A. P. (1991) · Cell · doi:10.1016/0092-8674(91)90267-3 · Cited by 733 · see citing works
Self-citationLoss of genomic methylation causes p53-dependent apoptosis and epigenetic deregulation
Jackson-Grusby, L., Beard, C., Possemato, R., Tudor, M., Fambrough, D., Csankovszki, G., Dausman, J., Lee, P., Wilson, C., Lander, E., et al. (2001) · Nature Genetics · doi:10.1038/83730 · Cited by 694 · see citing works
Identification of a mammalian protein that binds specifically to DNA containing methylated CpGs
Meehan, R. R., Lewis, J., McKay, S. J., Kleiner, E., & Bird, A. P. (1989) · Cell · doi:10.1016/0092-8674(89)90430-3 · Cited by 659 · see citing works
Self-citationDissection of the methyl-CpG binding domain from the chromosomal protein MeCP2
Nan, X., Meehan, R. R., & Bird, A. P. (1993) · Nucleic Acids Research · doi:10.1093/nar/21.21.4886 · Cited by 605 · see citing works
Self-citationClosely related proteins MBD2 and MBD3 play distinctive but interacting roles in mouse developmentPreprint
Hendrich, B., Guy, J., Ramsahoye, B. H., Wilson, V. A., & Bird, A. P. (2001) · Genes & Development · doi:10.1101/gad.194101 · Cited by 526 · see citing works
Self-citationInhibition of RhoA by p120 catenin
Anastasiadis, P. Z., Moon, S. Y., Thoreson, M. A., Mariner, D. J., Crawford, H. C., Zheng, Y., & Reynolds, A. B. (2000) · Nature Cell Biology · doi:10.1038/35023588 · Cited by 472 · see citing works
The p120 catenin family: Complex roles in adhesion, signaling and cancer
Anastasiadis, P. Z., & Reynolds, A. B. (2000) · Journal of Cell Science · doi:10.1242/jcs.113.8.1319 · Cited by 431 · see citing works
The Catenin p120 ctn Interacts with Kaiso, a Novel BTB/POZ Domain Zinc Finger Transcription Factor
Daniel, J. M., & Reynolds, A. B. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.5.3614 · Cited by 406 · see citing works
Graff, J. R., Gabrielson, E. W., Fujii, H., Baylin, S. B., & Herman, J. G. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.275.4.2727 · Cited by 393 · see citing works
Cytosine methylation and mammalian developmentPreprint
Walsh, C. P., & Bestor, T. H. (1999) · Genes & Development · doi:10.1101/gad.13.1.26 · Cited by 389 · see citing works
Dann, C. T., Subramaniam, K., Dunn, M. A., & Seydoux, G. (2001) · Genes & Development · doi:10.1101/gad.876201 · Cited by 357 · see citing works
All in the Family: the BTB/POZ, KRAB, and SCAN Domains
Collins, T., Stone, J. R., & Williams, A. J. (2001) · Molecular and Cellular Biology · doi:10.1128/mcb.21.11.3609-3615.2001 · Cited by 345 · see citing works
DNA Methylation Specifies Chromosomal Localization of MeCP2
Nan, X., Tate, P. H., Li, E., & Bird, A. P. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.1.414 · Cited by 343 · see citing works
Self-citationThe BCL-6 POZ domain and other POZ domains interact with the co-repressors N-CoR and SMRT
Huynh, K. D., & Bardwell, V. J. (1998) · Oncogene · doi:10.1038/sj.onc.1202197 · Cited by 311 · see citing works
Active Repression of Methylated Genes by the Chromosomal Protein MBD1
Ng, H., Jeppesen, P., & Bird, A. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.4.1394-1406.2000 · Cited by 292 · see citing works
Self-citationSinger–Sam, J., Grant, M., LeBon, J. M., et al. (1990) · Molecular and Cellular Biology · doi:10.1128/mcb.10.9.4987 · Cited by 181 · see citing works
van Hengel, J., Vanhoenacker, P., Staes, K., & van Roy, F. (1999) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.96.14.7980 · Cited by 160 · see citing works
Tenenhaus, C. (2020) · Carolina Digital Repository (University of North Carolina at Chapel Hill) · doi:10.17615/9024-dx23 · Cited by 150 · see citing works
DNA Recognition by the Methyl-CpG Binding Domain of MeCP2
Free, A., Wakefield, R. I. D., Smith, B. O., Dryden, D. T. F., Barlow, P. N., & Bird, A. P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m007224200 · Cited by 122 · see citing works
Self-citationFiore, B. D. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.14.2852 · Cited by 36 · see citing works
Recognition of DMA methylation by zinc fingers
Choo, Y. (1998) · Nature Structural Biology · doi:10.1038/nsb0498-264 · Cited by 26 · see citing works
Huntriss, J., Lorenzi, R., Purewal, A., et al. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.6876 · Cited by 20 · see citing works
Prokhortchouk, E., Prokhortchouk, A., Rouzov, A. S., et al. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.1857 · Cited by 13 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 1
- Kadonaga, J.T. 1996. Purification of transcription factor AP-1 from HeLa cells. In Strategies for protein purification and characterization (ed. D. Marshak et al.) pp. 127-204. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Cited by
Sorted by citations
DNA methylation patterns and epigenetic memory
Bird, A. (2002) · Genes & Development · doi:10.1101/gad.947102 · Cited by 7,124 · see citing works
Self-citationJaenisch, R., & Bird, A. (2003) · Nature Genetics · doi:10.1038/ng1089 · Cited by 6,325 · see citing works
Self-citationDNA methylation and its basic function
Moore, L. D., Le, T., & Fan, G. (2013) · Neuropsychopharmacology · doi:10.1038/npp.2012.112 · Cited by 4,906 · see citing works
Chromatin modification and epigenetic reprogramming in mammalian development
Li, E. (2002) · Nature Reviews Genetics · doi:10.1038/nrg887 · Cited by 1,900 · see citing works
Targeting epigenetic regulators for cancer therapy: mechanisms and advances in clinical trials
Cheng, Y., He, C., Wang, M., Ma, X., Mo, F., Yang, S., Han, J., & Wei, X. (2019) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-019-0095-0 · Cited by 1,097 · see citing works
TETonic shift: biological roles of TET proteins in DNA demethylation and transcription
Pastor, W. A., Aravind, L., & Rao, A. (2013) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm3589 · Cited by 897 · see citing works
Epigenetic mechanisms in memory formation
Levenson, J. M., & Sweatt, J. D. (2005) · Nature Reviews Neuroscience · doi:10.1038/nrn1604 · Cited by 767 · see citing works
Transcription factors as readers and effectors of DNA methylation
Zhu, H., Wang, G., & Qian, J. (2016) · Nature Reviews Genetics · doi:10.1038/nrg.2016.83 · Cited by 649 · see citing works
Methyl-cpG-binding domain proteins: readers of the epigenome
Du, Q., Luu, P.-L., Stirzaker, C., & Clark, S. J. (2015) · Epigenomics · doi:10.2217/epi.15.39 · Cited by 544 · see citing works
N-CoR Mediates DNA Methylation-Dependent Repression through a Methyl CpG Binding Protein Kaiso
Yoon, H.-G., Chan, D. W., Reynolds, A. B., Qin, J., & Wong, J. (2003) · Molecular Cell · doi:10.1016/j.molcel.2003.08.008 · Cited by 408 · see citing works
Sensitivity of transcription factors to DNA methylation
Héberlé, É., & Bardet, A. F. (2019) · Essays in Biochemistry · doi:10.1042/ebc20190033 · Cited by 347 · see citing works
Role of BDNF epigenetics in activity-dependent neuronal plasticity
Karpova, N. N. (2014) · Neuropharmacology · doi:10.1016/j.neuropharm.2013.04.002 · Cited by 200 · see citing works
Zhao, C., Deng, Y., Liu, L., Yu, K., Zhang, L., Wang, H., He, X., Wang, J., Lu, C., Wu, L. N., et al. (2016) · Nature Communications · doi:10.1038/ncomms10883 · Cited by 154 · see citing works
Buck-Koehntop, B. A., Stanfield, R. L., Ekiert, D. C., Martinez-Yamout, M. A., Dyson, H. J., Wilson, I. A., & Wright, P. E. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1213726109 · Cited by 128 · see citing works
Lopes, E. C., Valls, E., Figueroa, M. E., Mazur, A., Meng, F.-G., Chiosis, G., Laird, P. W., Schreiber-Agus, N., Greally, J. M., Prokhortchouk, E., et al. (2008) · Cancer Research · doi:10.1158/0008-5472.can-08-0344 · Cited by 119 · see citing works
Self-citationStructural basis for Klf4 recognition of methylated DNA
Liu, Y., Olanrewaju, Y. O., Zheng, Y., Hashimoto, H., Blumenthal, R. M., Zhang, X., & Cheng, X. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku134 · Cited by 99 · see citing works
ZBTB33 binds unmethylated regions of the genome associated with actively expressed genes
Blattler, A., Yao, L., Wang, Y., Ye, Z., Jin, V. X., & Farnham, P. J. (2013) · Epigenetics & Chromatin · doi:10.1186/1756-8935-6-13 · Cited by 82 · see citing works
Hantusch, B., Kalt, R., Krieger, S., Puri, C., & Kerjaschki, D. (2007) · BMC Molecular Biology · doi:10.1186/1471-2199-8-20 · Cited by 63 · see citing works
Epigenetic regulation of key vascular genes and growth factors
Turunen, M. P., & Yla-Herttuala, S. (2011) · Cardiovascular Research · doi:10.1093/cvr/cvr109 · Cited by 41 · see citing works
Karlsson, Q. H., Schelcher, C., Verrall, E., Petosa, C., & Sinclair, A. J. (2008) · PLoS Pathogens · doi:10.1371/journal.ppat.1000005 · Cited by 39 · see citing works
- Authors
- Prokhortchouk, A., Hendrich, B., Jørgensen, H., Ruzov, A., Wilm, M., Georgiev, G., Bird, A., & Prokhortchouk, E.
- Affiliations
- Institute of Gene Biology (RU)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2001-07-01
- Volume
- 15
- Issue
- 13
- Pages
- 1613-1618
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- PMC
- PMC312733
- OpenAlex
- W2163311832
- Cited by
- 533
- References
- 32
- Citations by year
- 2026: 10 · 2025: 5 · 2024: 26 · 2023: 19 · 2022: 24 · 2021: 11 · 2020: 10 · 2019: 18 · 2018: 19 · 2017: 24 · 2016: 21 · 2015: 22 · 2014: 25 · 2013: 32 · 2012: 26
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Keywords
- Kaiso, p120 catenin, DNA methylation, transcriptional repression, CpG, methyl-CpG binding proteins, zinc finger, POZ domain, gene silencing, DNA binding specificity
- MeSH terms
- Delta Catenin, Animals, Cell Extracts, Cell Line, Cell Nucleus, DNA-Binding Proteins, Histone Deacetylases, Humans, Phosphoproteins, Rabbits, Repressor Proteins, Transcription Factors, Transcription, Genetic, Signal Transduction, Cell Adhesion Molecules, Zinc Fingers, CpG Islands, DNA Methylation, Catenins, Mice
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