PreprintCited by 533Epigenetics and DNA MethylationGenomics and Chromatin DynamicsWnt/β-catenin signaling in development and cancer

The p120 catenin partner Kaiso is a DNA methylation-dependent transcriptional repressor

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Prokhortchouk, Anna, Hendrich, Brian, Jørgensen, Helle, Ruzov, Alexei, Wilm, Matthias, Georgiev, Georgii, Bird, Adrian, Prokhortchouk, Egor·2001·Genes & Development

Abstract

We describe a novel mammalian DNA binding activity that requires at least two symmetrically methylated CpG dinucleotides in its recognition sequence, preferably within the sequence 5′CGCG. A key component of the activity is Kaiso, a protein with POZ and zinc-finger domains that is known to associate with p120 catenin. We find that Kaiso behaves as a methylation-dependent transcriptional repressor in transient transfection assays. Kaiso is a constituent of one of two methyl–CpG binding complexes originally designated as MeCP1. The data suggest that zinc-finger motifs are responsible for DNA binding, and may therefore target repression to specific methylated regions of the genome. As Kaiso associates with p120 catenin, Kaiso may link events at the cell surface with DNA methylation-dependent gene silencing.

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  • Kaiso binds a specific methylated sequence through its C2H2 zinc-finger domains.

    “the transcriptional regulator Kaiso, which contains POZ (pox virus and zinc-finger) and zinc-finger domains, was found to bind to a specific methylated sequence with its C2H2 zinc-finger domains41”

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  1. Kadonaga, J.T. 1996. Purification of transcription factor AP-1 from HeLa cells. In Strategies for protein purification and characterization (ed. D. Marshak et al.) pp. 127-204. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

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Authors
Prokhortchouk, A., Hendrich, B., Jørgensen, H., Ruzov, A., Wilm, M., Georgiev, G., Bird, A., & Prokhortchouk, E.
Affiliations
Institute of Gene Biology (RU)
Venue
Genes & Development
Published
2001-07-01
Volume
15
Issue
13
Pages
1613-1618
ISSN
  • 0890-9369 (ISSN-L)
  • 1549-5477
OpenAlex
W2163311832
Cited by
533
References
32
Citations by year
2026: 10 · 2025: 5 · 2024: 26 · 2023: 19 · 2022: 24 · 2021: 11 · 2020: 10 · 2019: 18 · 2018: 19 · 2017: 24 · 2016: 21 · 2015: 22 · 2014: 25 · 2013: 32 · 2012: 26
Type
posted content
Language
en
Open access
Locations
  • Genes & Development (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Wellcome Trust
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
Keywords
Kaiso, p120 catenin, DNA methylation, transcriptional repression, CpG, methyl-CpG binding proteins, zinc finger, POZ domain, gene silencing, DNA binding specificity
MeSH terms
Delta Catenin, Animals, Cell Extracts, Cell Line, Cell Nucleus, DNA-Binding Proteins, Histone Deacetylases, Humans, Phosphoproteins, Rabbits, Repressor Proteins, Transcription Factors, Transcription, Genetic, Signal Transduction, Cell Adhesion Molecules, Zinc Fingers, CpG Islands, DNA Methylation, Catenins, Mice

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