Sensitivity of transcription factors to DNA methylation
Open accessAbstract
Dynamic binding of transcription factors (TFs) to regulatory elements controls transcriptional states throughout organism development. Epigenetics modifications, such as DNA methylation mostly within cytosine-guanine dinucleotides (CpGs), have the potential to modulate TF binding to DNA. Although DNA methylation has long been thought to repress TF binding, a more recent model proposes that TF binding can also inhibit DNA methylation. Here, we review the possible scenarios by which DNA methylation and TF binding affect each other. Further in vivo experiments will be required to generalize these models.
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When a recruited transcription factor is inhibitory, DNA methylation results in enhanced gene expression.
“When a recruited transcription factor is inhibitory, DNA methylation results in enhanced gene expression.58”
From Epigenetic regulation in major depression and other stress-related disorders: molecular mechanisms, clinical relevance and therapeutic potential (2023) · cites this paper as [58]
In ‘DNA methylation represses TF binding’ (page unavailable), the review describes a specific case: methylation prevents the insulating factor CTCF from binding at the mouse Igf2-H19 locus, allowing an enhancer to activate Igf2. This supports the proposed mechanism, but not the unqualified claim that methylation enhances gene expression whenever an inhibitory transcription factor is involved; the review says the functional effects of methylation-sensitive binding remain to be investigated more broadly. (LLM, conf=0.83)
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Deciphering the dynamic code: DNA recognition by transcription factors in the ever-changing genome
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- Authors
- Héberlé, É., & Bardet, A. F.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Bardet, Anaïs Flore
- Venue
- Essays in Biochemistry
- Publisher
- Portland Press Ltd.
- Published
- 2019-11-22
- Volume
- 63
- Issue
- 6
- Pages
- 727-741
- ISSN
- 0071-1365 (ISSN-L)
- 1744-1358
- PMC
- PMC6923324
- OpenAlex
- W2990086739
- Cited by
- 347
- References
- 99
- Citations by year
- 2026: 30 · 2025: 45 · 2024: 82 · 2023: 61 · 2022: 69 · 2021: 38 · 2020: 20 · 2019: 1
- FWCI
- 2.83
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Essays in Biochemistry (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Funders
- Agence Nationale de la Recherche (ANR-10-IDEX-0002)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Keywords
- transcription factors, DNA methylation, CpG, transcription factor binding, epigenetic regulation, transcriptional repression, regulatory elements, epigenetic inheritance
- MeSH terms
- Animals, DNA, Humans, Protein Binding, Transcription Factors, DNA Methylation, Epigenesis, Genetic
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