The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
AphA and LuxR/HapR reciprocally control quorum sensing in vibrios
Open accessAbstract
Bacteria cycle between periods when they perform individual behaviors and periods when they perform group behaviors. These transitions are controlled by a cell–cell communication process called quorum sensing, in which extracellular signal molecules, called autoinducers (AIs), are released, accumulate, and are synchronously detected by a group of bacteria. AI detection results in community-wide changes in gene expression, enabling bacteria to collectively execute behaviors such as bioluminescence, biofilm formation, and virulence factor production. In this study, we show that the transcription factor AphA is a master regulator of quorum sensing that operates at low cell density (LCD) in Vibrio harveyi and Vibrio cholerae. In contrast, LuxR (V. harveyi)/HapR (V. cholerae) is the master regulator that operates at high cell density (HCD). At LCD, redundant small noncoding RNAs (sRNAs) activate production of AphA, and AphA and the sRNAs repress production of LuxR/HapR. Conversely, at HCD, LuxR/HapR represses aphA. This network architecture ensures maximal AphA production at LCD and maximal LuxR/HapR production at HCD. Microarray analyses reveal that 300 genes are regulated by AphA at LCD in V. harveyi, a subset of which is also controlled by LuxR. We propose that reciprocal gradients of AphA and LuxR/HapR establish the quorum-sensing LCD and HCD gene expression patterns, respectively.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
Datsenko, K. A., & Wanner, B. L. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.120163297 · Cited by 14,782
The Involvement of Cell-to-Cell Signals in the Development of a Bacterial Biofilm
Davies, D. G., Parsek, M. R., Pearson, J. P., Iglewski, B. H., Costerton, J. W., & Greenberg, E. P. (1998) · Science · doi:10.1126/science.280.5361.295 · Cited by 3,396
Casadaban, M. J. (1976) · Journal of Molecular Biology · doi:10.1016/0022-2836(76)90119-4 · Cited by 1,872
Bacterial Quorum-Sensing Network Architectures
Ng, W.-L., & Bassler, B. L. (2009) · Annual Review of Genetics · doi:10.1146/annurev-genet-102108-134304 · Cited by 1,679
Self-citationEngineering stability in gene networks by autoregulation
Becskei, A., & Serrano, L. (2000) · Nature · doi:10.1038/35014651 · Cited by 1,520
Autoinduction and signal transduction in the regulation of staphylococcal virulence
Novick, R. P. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03526.x · Cited by 1,280
Lenz, D. H., Mok, K. C., Lilley, B. N., Kulkarni, R. V., Wingreen, N. S., & Bassler, B. L. (2004) · Cell · doi:10.1016/j.cell.2004.06.009 · Cited by 971
Self-citationQuorum sensing controls biofilm formation in Vibrio cholerae
Hammer, B. K., & Bassler, B. L. (2003) · Molecular Microbiology · doi:10.1046/j.1365-2958.2003.03688.x · Cited by 886
Self-citationde Lorenzo, V., & Timmis, K. N. (1994) · Methods in Enzymology · doi:10.1016/0076-6879(94)35157-0 · Cited by 879
Cited by
Quorum sensing signal-response systems in Gram-negative bacteria
Papenfort, K., & Bassler, B. L. (2016) · Nature Reviews Microbiology · doi:10.1038/nrmicro.2016.89 · Cited by 2,256
Self-citationBacterial quorum sensing in complex and dynamically changing environments
Mukherjee, S., & Bassler, B. L. (2019) · Nature Reviews Microbiology · doi:10.1038/s41579-019-0186-5 · Cited by 1,374
Self-citationA quorum-sensing inhibitor blocks Pseudomonas aeruginosa virulence and biofilm formation
O’Loughlin, C. T., Miller, L. C., Siryaporn, A., Drescher, K., Semmelhack, M. F., & Bassler, B. L. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1316981110 · Cited by 744
Self-citationCuthbertson, L., & Nodwell, J. R. (2013) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00018-13 · Cited by 554
Regulation of Bacterial Virulence by Csr (Rsm) Systems
Vakulskas, C. A., Potts, A. H., Babitzke, P., Ahmer, B. M. M., & Romeo, T. (2015) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00052-14 · Cited by 346
Metadata
- Authors
- Rutherford, S. T., van Kessel, J. C., Shao, Y., & Bassler, B. L.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2011-02-15
- Volume
- 25
- Issue
- 4
- Pages
- 397-408
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2149311070
- Cited by
- 293
- References
- 51
- Type
- posted content
- Language
- en
- Open access
- diamond
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: AphA and LuxR/HapR reciprocally control quorum sensing in vibrios. https://app.cochat.ai/papers/10.1101/gad.2015011
- The paper
- Rutherford, S. T., van Kessel, J. C., Shao, Y., & Bassler, B. L. (2011). AphA and LuxR/HapR reciprocally control quorum sensing in vibrios. Genes & Development. https://doi.org/10.1101/gad.2015011
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
