The DOI matches its Crossref record; 40 of the 40 references it cites were found in a registry or an index.
Coactivators for a proline-rich activator purified from the multisubunit human TFIID complex.
Open accessAbstract
The mechanisms of transcriptional activation directed by sequence-specific regulators is central to understanding gene regulation. Here, we report the isolation of coactivators responsible for mediating transcriptional activation by Gal4-Pro, a hybrid regulator containing the proline-rich activation domain of human CTF/NFI. Chromatographic studies indicate that endogenous human TFIID consists of a multisubunit complex containing the TATA-binding protein (TBP), coactivators, and other associated factors. A fraction containing the coactivator activity was separated from the endogenous TBP after disrupting the tightly associated complex with urea. The urea-purified TBP was active for basal level transcription but no longer could support activation by Gal4-Pro. However, when the two separated components were added together, activated levels of transcription were restored in the presence of Gal4-Pro. Immunoaffinity purification of the TFIID complex identifies several polypeptides specifically associated with the endogenous TBP, some or all of which function as coactivators when reconstituted with Gal4-Pro. The isolated coactivators also mediate activation by a chimeric glutamine-rich activator derived from Sp1 but not the Gal4-VP16 activator, suggesting distinct factor requirements for different types of transcriptional regulators.
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Citations
Cites
Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins
Mitchell, P. J., & Tjian, R. (1989) · Science · doi:10.1126/science.2667136 · Cited by 3,114
Self-citationCourey, A. J., & Tjian, R. (1988) · Cell · doi:10.1016/0092-8674(88)90144-4 · Cited by 1,354
Self-citationFive intermediate complexes in transcription initiation by RNA polymerase II
Buratowski, S., Hahn, S., Guarente, L., & Sharp, P. A. (1989) · Cell · doi:10.1016/0092-8674(89)90578-3 · Cited by 1,027
A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication
Jones, K. A., Kadonaga, J. T., Rosenfeld, P. J., Kelly, T. J., & Tjian, R. (1987) · Cell · doi:10.1016/0092-8674(87)90358-8 · Cited by 1,002
Self-citationMechanism of transcriptional activation by Sp1: Evidence for coactivators
Pugh, B. F., & Tjian, R. (1990) · Cell · doi:10.1016/0092-8674(90)90683-6 · Cited by 1,001
Self-citationMermod, N., O'Neill, E. A., Kelly, T. J., & Tjian, R. (1989) · Cell · doi:10.1016/0092-8674(89)90108-6 · Cited by 764
Self-citationSantoro, C., Mermod, N., Andrews, P. C., & Tjian, R. (1988) · Nature · doi:10.1038/334218a0 · Cited by 715
Self-citationDynlacht, B. D., Hoey, T., & Tjian, R. (1991) · Cell · doi:10.1016/0092-8674(81)90019-2 · Cited by 683
Self-citationTranscription activation by the adenovirus E1a protein
Lillie, J. W., & Green, M. R. (1989) · Nature · doi:10.1038/338039a0 · Cited by 632
Keegan, L., Gill, G., & Ptashne, M. (1986) · Science · doi:10.1126/science.3080805 · Cited by 612
Cited by
Seipel, K., Georgiev, O., & Schaffner, W. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05603.x · Cited by 349
Functional domains of the Drosophila Engrailed protein.
Han, K., & Manley, J. L. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb05934.x · Cited by 272
Sanchez, H. B., Yieh, L., & Osborne, T. F. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.3.1161 · Cited by 257
Neely, K. E., Hassan, A. H., Wallberg, A. E., Steger, D. J., Cairns, B. R., Wright, A. P. H., & Workman, J. L. (1999) · Molecular Cell · doi:10.1016/s1097-2765(00)80216-6 · Cited by 245
Guermah, M., Malik, S., & Roeder, R. G. (1998) · Molecular and Cellular Biology · doi:10.1128/mcb.18.6.3234 · Cited by 91
Metadata
- Authors
- Tanese, N., Pugh, B. F., & Tjian, R.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1991-12-01
- Volume
- 5
- Issue
- 12a
- Pages
- 2212-2224
- ISSN
- 0890-9369 (ISSN-L)
- OpenAlex
- W2140134113
- Cited by
- 346
- References
- 40
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Tanese, N., Pugh, B. F., & Tjian, R. (1991). Coactivators for a proline-rich activator purified from the multisubunit human TFIID complex. Genes & Development. https://doi.org/10.1101/gad.5.12a.2212
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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