Cited by 245Chromatin Remodeling and CancerGenomics and Chromatin DynamicsNuclear Structure and Function

Activation Domain–Mediated Targeting of the SWI/SNF Complex to Promoters Stimulates Transcription from Nucleosome Arrays

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Neely, Kristen E, Hassan, Ahmed H, Wallberg, Annika E, Steger, David J, Cairns, Bradley R, Wright, Anthony P.H, Workman, Jerry L·1999·Molecular Cell

Abstract

The yeast SWI/SNF complex is required for the transcription of several yeast genes and has been shown to alter nucleosome structure in an ATP-dependent reaction. In this study, we show that the complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner. Transcription stimulation by SWI/SNF required an activation domain with which it directly interacts. The acidic activation domains of VP16, Gcn4, Swi5, and Hap4 interacted directly with the purified SWI/SNF complex and with the SWI/SNF complex in whole-cell extracts. The similarity of activation domain interactions and transcriptional stimulation between SWI/SNF and the SAGA histone acetyltransferase complex may account for their apparent overlapping functions in vivo.

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Crossref · 10.1016/s1097-2765(00)80216-6

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Authors
Neely, K. E., Hassan, A. H., Wallberg, A. E., Steger, D. J., Cairns, B. R., Wright, A. P. H., & Workman, J. L.
Affiliations
  • Pennsylvania State University (US)
  • Södertörn University (SE)
  • University of Utah (US)
Corresponding author
Workman, Jerry L
Venue
Molecular Cell
Publisher
Elsevier BV
Published
1999-10
Volume
4
Issue
4
Pages
649-655
ISSN
1097-2765 (ISSN-L)
OpenAlex
W2119383396
Cited by
245
References
53
Citations by year
2026: 3 · 2025: 2 · 2024: 8 · 2023: 3 · 2022: 5 · 2021: 6 · 2020: 7 · 2019: 2 · 2018: 5 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 8 · 2013: 2 · 2012: 5
FWCI
9.13
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
SWI/SNF complex, chromatin remodeling, transactivation domain, nucleosome arrays, transcription initiation, nucleosome structure, SAGA complex, histone acetyltransferase, protein-protein interaction, Swi5, HAP4, GCN4, VP16
MeSH terms
Chromatin, DNA-Binding Proteins, Fungal Proteins, Nuclear Proteins, Nucleosomes, Promoter Regions, Genetic, Protein Kinases, Recombinant Proteins, Transcription Factors, Yeasts, Transcriptional Activation, Gene Expression Regulation, Fungal, Herpes Simplex Virus Protein Vmw65, Cell Cycle Proteins, CCAAT-Binding Factor, Saccharomyces cerevisiae Proteins

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