The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes
Open accessAbstract
Alteration of nucleosomes by ATP-dependent remodeling complexes represents a critical step in the regulation of transcription. The human SWI/SNF (hSWI/SNF) family is composed of complexes that contain either Brg1 or hBrm as the central ATPase; however, these separate complexes have not been compared functionally. Here we describe the establishment of cell lines that express epitope-tagged Brg1 and hBrm and a characterization of the complexes associated with these two ATPases. We show that Brg1 fractionates into two complexes that differ in activity and subunit composition, whereas hBrm is found in one complex with lower activity than the Brg1 complexes. These three complexes can remodel nucleosomal arrays, increase restriction enzyme accessibility, and hydrolyze ATP in a DNA-dependent manner. The three complexes differ markedly in their ability to remodel mononucleosomal core particles. We also show that the hBrm complex and one of the Brg1 complexes contain components of the mammalian Sin3 (mSin3) complex. In addition, we have found that Brg1, hBrm, and BAF155 can interact specifically with mSin3A in vitro, showing a direct association of hSWI/SNF complexes with proteins involved in gene repression. These unexpected functional characteristics indicate that these hSWI/SNF complexes play diverse regulatory roles.
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Cites
Histone acetylation in chromatin structure and transcription
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Nuclear Receptor Repression Mediated by a Complex Containing SMRT, mSin3A, and Histone Deacetylase
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Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex
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Self-citationIkaros DNA-Binding Proteins Direct Formation of Chromatin Remodeling Complexes in Lymphocytes
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Self-citationHistone Deacetylases and SAP18, a Novel Polypeptide, Are Components of a Human Sin3 Complex
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Rosenfeld, M. G., Lunyak, V. V., & Glass, C. K. (2006) · Genes & Development · doi:10.1101/gad.1424806 · Cited by 952
Nakamura, T., Mori, T., Tada, S., Krajewski, W., Rozovskaia, T., Wassell, R., Dubois, G., Mazo, A., Croce, C. M., & Canaani, E. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00740-2 · Cited by 721
Rayman, J. B., Takahashi, Y., Indjeian, V. B., Dannenberg, J.-H., Catchpole, S., Watson, R. J., te Riele, H., & Dynlacht, B. D. (2002) · Genes & Development · doi:10.1101/gad.969202 · Cited by 288
Metadata
- Authors
- Sif, S., Saurin, A. J., Imbalzano, A. N., & Kingston, R. E.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2001-03-01
- Volume
- 15
- Issue
- 5
- Pages
- 603-618
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2119855916
- Cited by
- 283
- References
- 7
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Sif, S., Saurin, A. J., Imbalzano, A. N., & Kingston, R. E. (2001). Purification and characterization of mSin3A-containing Brg1 and hBrm chromatin remodeling complexes. Genes & Development. https://doi.org/10.1101/gad.872801
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