Histone Deacetylases and SAP18, a Novel Polypeptide, Are Components of a Human Sin3 Complex
Open accessAbstract
An important event in gene expression is the covalent modification of histone proteins. We have found that the mammalian transcriptional repressor Sin3 (mSin3) exists in a complex with histone deacetylases HDAC1 and HDAC2. Consistent with the observation that mSin3-mediated repression of transcription involves the modification of histone polypeptides, we found that the mSin3-containing complex includes polypeptides that tether the mSin3 complex to core histone proteins. In addition, two novel mSin3-associated polypeptides, SAP18 and SAP30, were identified. We isolated a cDNA encoding human SAP18 and found that SAP18 is a component of an mSin3-containing complex in vivo. Moreover, we demonstrate a direct interaction between SAP18 and mSin3. SAP18 represses transcription in vivo when tethered to the promoter, consistent with the ability of SAP18 to interact with mSin3.
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RBBP4 and RBBP7 are present in many protein complexes other than NuRD (Sin3 complex, PRC2).
“RBBP4 and RBBP7 are present in many protein complexes other than the NuRD complex (Sin3 complex, PRC2), which also could explain their observed dynamic behavior [21,22].”
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- Authors
- Zhang, Y., Iratni, R., Erdjument-Bromage, H., Tempst, P., & Reinberg, D.
- Affiliations
- Howard Hughes Medical Institute (US)
- Memorial Sloan Kettering Cancer Center (US)
- Corresponding author
- Reinberg, Danny
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1997-05
- Volume
- 89
- Issue
- 3
- Pages
- 357-364
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2074096799
- Cited by
- 578
- References
- 44
- Citations by year
- 2026: 6 · 2025: 10 · 2024: 10 · 2023: 14 · 2022: 8 · 2021: 14 · 2020: 14 · 2019: 11 · 2018: 10 · 2017: 8 · 2016: 15 · 2015: 11 · 2014: 18 · 2013: 14 · 2012: 6
- FWCI
- 19.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (2r01gm048518-05)
- National Cancer Institute (5P309CA08748)
- National Institute of General Medical Sciences (GM37120, GM-48518)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Histone Deacetylase Inhibitors Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- histone deacetylase, HDAC1, HDAC2, SAP18, SAP30, transcriptional repression, histone deacetylation, histone modifications, protein-protein interaction, core histones, transcriptional repressor
- MeSH terms
- Animals, Carrier Proteins, Cell Fractionation, HeLa Cells, Histone Deacetylases, Humans, Mammals, Molecular Sequence Data, Multienzyme Complexes, Nuclear Proteins, Precipitin Tests, Repressor Proteins, Retinoblastoma, Transcription Factors, Transcription, Genetic, Blotting, Western, RNA-Binding Proteins, Sequence Homology, Amino Acid, Saccharomyces cerevisiae Proteins, Retinoblastoma-Binding Protein 4, Retinoblastoma-Binding Protein 7, Co-Repressor Proteins
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