The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.
Poly(A) site selection in the HIV-1 provirus: inhibition of promoter-proximal polyadenylation by the downstream major splice donor site
Open accessAbstract
In common with all retroviruses, the human immunodeficiency virus type 1 (HIV-1) contains duplicated long terminal repeat (LTR) sequences flanking the proviral genome. These LTRs contain identical poly(A) signals, which are both transcribed into RNA. Therefore, to allow efficient viral expression, a mechanism must exist to either restrict promoter-proximal poly(A) site use or enhance the activity of the promoter-distal poly(A) site. We have examined the use of both poly(A) sites using proviral clones. Mutation of the previously defined upstream activatory sequences of the 3' LTR poly(A) site decreases the efficiency of polyadenylation when placed in competition with an efficient downstream processing signal. However, in the absence of competition, these mutations have no effect on HIV-1 polyadenylation. In addition, the 5' LTR poly(A) site is inactive, whereas a heterologous poly(A) site positioned in its place is utilized efficiently. Furthermore, transcription initiating from the 3' LTR promoter utilizes the 3' LTR poly(A) signal efficiently. Therefore, the main determinant of the differential poly(A) site use appears to be neither proximity to a promoter element in the 5' LTR nor the presence of upstream activating sequences at the 3' LTR. Instead, we show that the major splice donor site that is immediately downstream of the 5' LTR inhibits cleavage and polyadenylation at the promoter-proximal site. The fact that this poly(A) site is active in a proviral clone when the major splice donor site is mutated suggests that the selective use of poly(A) signals in HIV-1 is mediated by a direct inhibition of the HIV-1 poly(A) site by downstream splicing events or factors involved in splicing.
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“Furthermore, in both HIV-1 and foamy viruses, the primary factor in splice-site-mediated suppression is the binding of U1 small nuclear ribonucleoprotein (U1 snRNP), part of the spliceosome complex, to the major splice donor site (Ashe et al. 1995, 1997, 2000, Schrom et al. 2013).”
From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Ashe et al. 1995
In the citing paper
“These sequences have been shown to boost polyadenylation by improving CPSF binding both in vitro as well as in vivo (Brown et al. 1991, Valsamakis et al. 1992, Ashe et al. 1995, Gilmartin et al. 1995, Graveley and Gilmartin 1996).”
From Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication (2026) · cites this paper as Ashe et al. 1995
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“These sequences have been shown to boost polyadenylation by improving CPSF binding both in vitro as well as in vivo (Brown et al. 1991 , Valsamakis et al. 1992 , Ashe et al. 1995 , Gilmartin et al. 1995 , Graveley and Gilmartin 1996 ).”
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Metadata
- Authors
- Ashe, M. P., Griffin, P., James, W., & Proudfoot, N. J.
- Affiliations
- University of Oxford (GB)
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 1995-12-01
- Volume
- 9
- Issue
- 23
- Pages
- 3008-3025
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2108413560
- Cited by
- 125
- References
- 62
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 4 · 2023: 5 · 2022: 4 · 2021: 2 · 2019: 3 · 2018: 5 · 2017: 2 · 2016: 5 · 2015: 2 · 2014: 5 · 2013: 5 · 2012: 4
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genes & Development (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Funders
- Wellcome Trust
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- HIV-1, polyadenylation site selection, long terminal repeat, upstream activator sequence, 5' LTR, 3' LTR, retroviral transcription, polyadenylation signal
- MeSH terms
- Base Sequence, Binding, Competitive, Gene Expression Regulation, Humans, Molecular Sequence Data, Mutation, Poly A, Promoter Regions, Genetic, Proviruses, RNA Splicing, RNA, Messenger, Transcription, Genetic, HIV-1, HIV Long Terminal Repeat
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