The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells
Open accessAbstract
RNA interference (RNAi) was first recognized in Caenorhabditis elegans as a biological response to exogenous double-stranded RNA (dsRNA), which induces sequence-specific gene silencing. RNAi represents a conserved regulatory motif, which is present in a wide range of eukaryotic organisms. Recently, we and others have shown that endogenously encoded triggers of gene silencing act through elements of the RNAi machinery to regulate the expression of protein-coding genes. These small temporal RNAs (stRNAs) are transcribed as short hairpin precursors (∼70 nt), processed into active, 21-nt RNAs by Dicer, and recognize target mRNAs via base-pairing interactions. Here, we show that short hairpin RNAs (shRNAs) can be engineered to suppress the expression of desired genes in cultured Drosophila and mammalian cells. shRNAs can be synthesized exogenously or can be transcribed from RNA polymerase III promoters in vivo, thus permitting the construction of continuous cell lines or transgenic animals in which RNAi enforces stable and heritable gene silencing.
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Cites
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
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Oncogenic ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a
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BackgroundInfluentialIdentification of Novel Genes Coding for Small Expressed RNAs
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Self-citation
Cited by
Asymmetry in the Assembly of the RNAi Enzyme Complex
Schwarz, D. S., Hutvágner, G., Du, T., Xu, Z., Aronin, N., & Zamore, P. D. (2003) · Cell · doi:10.1016/s0092-8674(03)00759-1 · Cited by 2,922
Fantin, V. R., St-Pierre, J., & Leder, P. (2006) · Cancer Cell · doi:10.1016/j.ccr.2006.04.023 · Cited by 1,635
Reversal of human cellular senescence: roles of the p53 and p16 pathways
Beauséjour, C. M., Krtolica, A., Galimi, F., Narita, M., Lowe, S. W., Yaswen, P., & Campisi, J. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg417 · Cited by 1,457
The current state and future directions of RNAi-based therapeutics
Setten, R. L., Rossi, J. J., & Han, S.-P. (2019) · Nature Reviews Drug Discovery · doi:10.1038/s41573-019-0017-4 · Cited by 1,443
The Concept of Synthetic Lethality in the Context of Anticancer Therapy
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Metadata
- Authors
- Paddison, P. J., Caudy, A. A., Bernstein, E., Hannon, G. J., & Conklin, D. S.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2002-04-15
- Volume
- 16
- Issue
- 8
- Pages
- 948-958
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2153747430
- Cited by
- 1,552
- References
- 5
- Type
- posted content
- Language
- en
- Open access
- diamond
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- Paddison, P. J., Caudy, A. A., Bernstein, E., Hannon, G. J., & Conklin, D. S. (2002). Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes & Development. https://doi.org/10.1101/gad.981002
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