The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Targeted mutagenesis by homologous recombination in D. melanogaster
Open accessAbstract
We used a recently developed method to produce mutant alleles of five endogenous Drosophila genes, including the homolog of the p53 tumor suppressor. Transgenic expression of the FLP site-specific recombinase and the I- Sce I endonuclease generates extrachromosomal linear DNA molecules in vivo. These molecules undergo homologous recombination with the corresponding chromosomal locus to generate targeted alterations of the host genome. The results address several questions about the general utility of this technique. We show that genes not near telomeres can be efficiently targeted; that no knowledge of the mutant phenotype is needed for targeting; and that insertional mutations and allelic substitutions can be easily produced.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
DNA end-joining: from yeast to man
Critchlow, S. E., & Jackson, S. P. (1998) · Trends in Biochemical Sciences · doi:10.1016/s0968-0004(98)01284-5 · Cited by 584
Argast, G. M., Stephens, K. M., Emond, M. J., & Monnat, R. J. (1998) · Journal of Molecular Biology · doi:10.1006/jmbi.1998.1886 · Cited by 127 · cited 2×
“The differences that do exist have all been observed in substrate sequences that can be cut by this enzyme in vitro (Argast et al. 1998).”
Background
Cited by
The BDGP Gene Disruption Project
Bellen, H. J., Levis, R. W., Liao, G., He, Y., Carlson, J. W., Tsang, G., Evans-Holm, M., Hiesinger, P. R., Schulze, K. L., Rubin, G. M., et al. (2004) · Genetics · doi:10.1534/genetics.104.026427 · Cited by 873
Self-citationThe repair of double-strand breaks in plants: mechanisms and consequences for genome evolution
Puchta, H. (2004) · Journal of Experimental Botany · doi:10.1093/jxb/eri025 · Cited by 601
Construction of Transgenic Drosophila by Using the Site-Specific Integrase From Phage φC31
Groth, A. C., Fish, M., Nusse, R., & Calos, M. P. (2004) · Genetics · doi:10.1093/genetics/166.4.1775 · Cited by 423
A planarian p53 homolog regulates proliferation and self-renewal in adult stem cell lineages
Pearson, B. J., & Alvarado, A. S. (2010) · Development · doi:10.1242/dev.044297 · Cited by 188
Inositol phosphate kinase 2 is required for imaginal disc development in Drosophila
Seeds, A. M., Tsui, M. M., Sunu, C., Spana, E. P., & York, J. D. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1514684112 · Cited by 21
Metadata
- Authors
- Rong, Y. S., Titen, S. W., Xie, H. B., Golic, M. M., Bastiani, M., Bandyopadhyay, P., Olivera, B. M., Brodsky, M., Rubin, G. M., & Golic, K. G.
- Venue
- Genes & Development
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2002-06-15
- Volume
- 16
- Issue
- 12
- Pages
- 1568-1581
- ISSN
- 0890-9369 (ISSN-L)
- 1549-5477
- OpenAlex
- W2155995453
- Cited by
- 310
- References
- 2
- Type
- posted content
- Language
- en
- Open access
- diamond
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Targeted mutagenesis by homologous recombination in D. melanogaster. https://app.cochat.ai/papers/10.1101/gad.986602
- The paper
- Rong, Y. S., Titen, S. W., Xie, H. B., Golic, M. M., Bastiani, M., Bandyopadhyay, P., Olivera, B. M., Brodsky, M., Rubin, G. M., & Golic, K. G. (2002). Targeted mutagenesis by homologous recombination in D. melanogaster. Genes & Development. https://doi.org/10.1101/gad.986602
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
