A planarian p53 homolog regulates proliferation and self-renewal in adult stem cell lineages
Open accessAbstract
The functions of adult stem cells and tumor suppressor genes are known to intersect. However, when and how tumor suppressors function in the lineages produced by adult stem cells is unknown. With a large population of stem cells that can be manipulated and studied in vivo, the freshwater planarian is an ideal system with which to investigate these questions. Here, we focus on the tumor suppressor p53, homologs of which have no known role in stem cell biology in any invertebrate examined thus far. Planaria have a single p53 family member, Smed-p53, which is predominantly expressed in newly made stem cell progeny. When Smed-p53 is targeted by RNAi, the stem cell population increases at the expense of progeny, resulting in hyper-proliferation. However, ultimately the stem cell population fails to self-renew. Our results suggest that prior to the vertebrates, an ancestral p53-like molecule already had functions in stem cell proliferation control and self-renewal.
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p53 may take part in other essential physiological processes, such as stem cell homeostasis and fertility.
“There is increasing evidence that in addition to the ‘classical’ function of p53 in the DNA damage response, this versatile protein might be involved in other essential physiological processes, including stem cell homeostasis and fertility [106–108].”
From The p53 orchestra: Mdm2 and Mdmx set the tone (2010) · cites this paper as [107]
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- Authors
- Pearson, B. J., & Alvarado, A. S.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Alvarado, Alejandro Sánchez
- Venue
- Development
- Publisher
- The Company of Biologists
- Published
- 2010-01-15
- Volume
- 137
- Issue
- 2
- Pages
- 213-221
- ISSN
- 0950-1991 (ISSN-L)
- 1477-9129
- PMC
- PMC2799157
- OpenAlex
- W2093616637
- Cited by
- 188
- References
- 50
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 9 · 2023: 10 · 2022: 11 · 2021: 13 · 2020: 9 · 2019: 7 · 2018: 13 · 2017: 13 · 2016: 14 · 2015: 11 · 2014: 10 · 2013: 16 · 2012: 24
- FWCI
- 5.36
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Development (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- Damon Runyon Cancer Research Foundation
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- p53 homolog, Tricladida, adult stem cells, stem cell self-renewal, cell proliferation, tumor suppressor, RNAi knockdown, stem cell lineage
- MeSH terms
- Animals, Flow Cytometry, Immunohistochemistry, Planarians, Helminth Proteins, Tumor Suppressor Protein p53, In Situ Hybridization, Cell Lineage, RNA Interference, Adult Stem Cells
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