MicroRNA programs in normal and aberrant stem and progenitor cells
Open accessAbstract
Emerging evidence suggests that microRNAs (miRNAs), an abundant class of ∼22-nucleotide small regulatory RNAs, play key roles in controlling the post-transcriptional genetic programs in stem and progenitor cells. Here we systematically examined miRNA expression profiles in various adult tissue-specific stem cells and their differentiated counterparts. These analyses revealed miRNA programs that are common or unique to blood, muscle, and neural stem cell populations and miRNA signatures that mark the transitions from self-renewing and quiescent stem cells to proliferative and differentiating progenitor cells. Moreover, we identified a stem/progenitor transition miRNA (SPT-miRNA) signature that predicts the effects of genetic perturbations, such as loss of PTEN and the Rb family, AML1-ETO9a expression, and MLL-AF10 transformation, on self-renewal and proliferation potentials of mutant stem/progenitor cells. We showed that some of the SPT-miRNAs control the self-renewal of embryonic stem cells and the reconstitution potential of hematopoietic stem cells (HSCs). Finally, we demonstrated that SPT-miRNAs coordinately regulate genes that are known to play roles in controlling HSC self-renewal, such as Hoxb6 and Hoxa4. Together, these analyses reveal the miRNA programs that may control key processes in normal and aberrant stem and progenitor cells, setting the foundations for dissecting post-transcriptional regulatory networks in stem cells.
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miRNA profiling of HSCs, NSCs, and MuSCs and their differentiated progeny has led to a common miRNA signature of activation from quiescence.
“miRNA profiling of HSCs, NSCs and MuSCs and their differentiated progenies has led to a common miRNA signature of stem cell activation from quiescence”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [52]
miRNA pathways regulate stem cell quiescence post-transcriptionally.
“which suggests an important role of miRNA pathways in regulating stem cell quiescence post-transcriptionally52.”
From Molecular regulation of stem cell quiescence (2013) · cites this paper as [52]
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Molecular regulation of stem cell quiescence
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- Authors
- Arnold, C. P., Tan, R., Zhou, B., Yue, S.-B., Schaffert, S., Biggs, J. R., Doyonnas, R., Lo, M.-C., Perry, J. M., Renault, V. M., Sacco, A., Somervaille, T., Viatour, P., Brunet, A., Cleary, M. L., Li, L., Sage, J., Zhang, D.-E., Blau, H. M., Chen, C., & Chen, C.-Z.
- Affiliations
- Stanford Medicine (US)
- Stanford University (US)
- University of California San Diego (US)
- Stowers Institute for Medical Research (US)
- Sanford Burnham Prebys Medical Discovery Institute (US)
- ORCID
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2011-03-30
- Volume
- 21
- Issue
- 5
- Pages
- 798-810
- ISSN
- 1088-9051 (ISSN-L)
- PMC
- PMC3083097
- OpenAlex
- W2152264062
- Cited by
- 65
- References
- 56
- Citations by year
- 2026: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 4 · 2016: 5 · 2015: 11 · 2014: 10 · 2013: 12 · 2012: 8
- Type
- posted content
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Genome Research (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Funders
- W. M. Keck Foundation
- National Institutes of Health (NIH-R01, DP1OD006435, 5r01hl081612-05)
- National Institute on Aging (R01AG020961, P01AG036695)
- National Heart, Lung, and Blood Institute (R01-HL081612, U01HL100397)
- National Cancer Institute (DP1CA174421, R01CA104509)
- National Institute of Allergy and Infectious Diseases (R01AI073724)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- microRNA, stem cells, progenitor cells, microRNA signature, hematopoietic stem cells, embryonic stem cells, self-renewal, post-transcriptional regulation, PTEN loss, HOXB6, HOXA4, blood stem cells, muscle stem cells, neural stem cells, miRNA microarray
- MeSH terms
- Animals, Cell Differentiation, Gene Expression Regulation, Hematopoietic Stem Cells, Humans, Mutation, Organ Specificity, Stem Cells, Myoblasts, MicroRNAs, Mice, Embryonic Stem Cells, Neural Stem Cells
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