The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes
Open accessAbstract
In the effort to prepare the mouse full-length cDNA encyclopedia, we previously developed several techniques to prepare and select full-length cDNAs. To increase the number of different cDNAs, we introduce here a strategy to prepare normalized and subtracted cDNA libraries in a single step. The method is based on hybridization of the first-strand, full-length cDNA with several RNA drivers, including starting mRNA as the normalizing driver and run-off transcripts from minilibraries containing highly expressed genes, rearrayed clones, and previously sequenced cDNAs as subtracting drivers. Our method keeps the proportion of full-length cDNAs in the subtracted/normalized library high. Moreover, our method dramatically enhances the discovery of new genes as compared to results obtained by using standard, full-length cDNA libraries. This procedure can be extended to the preparation of full-length cDNA encyclopedias from other organisms.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Diatchenko, L., Lau, Y. F., Campbell, A. P., Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, E. D., et al. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.12.6025 · Cited by 3,010
Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project
Adams, M. D., Kelley, J. M., Gocayne, J. D., Dubnick, M., Polymeropoulos, M. H., Xiao, H., Merril, C. R., Wu, A., Olde, B., Moreno, R. F., et al. (1991) · Science · doi:10.1126/science.2047873 · Cited by 2,285
Adams, Kerlavage, A. R., Fleischmann, R. D., Ra, F., Cj, B., Lee, N., Ef, K., Kg, W., Gocayne, J. D., & White, O. R. (1995) · PubMed · Cited by 793
Kazuo, M., & Sumio, S. (1994) · Gene · doi:10.1016/0378-1119(94)90802-8 · Cited by 567
Normalization and subtraction: two approaches to facilitate gene discovery.
Bonaldo, M. F., Lennon, G., & Soares, M. B. (1996) · Genome Research · doi:10.1101/gr.6.9.791 · Cited by 522
Generation and analysis of 280,000 human expressed sequence tags.
Hillier, L. D., Lennon, G., Becker, M., Bonaldo, M. F., Chiapelli, B., Chissoe, S., Dietrich, N., DuBuque, T., Favello, A., Gish, W., et al. (1996) · Genome Research · doi:10.1101/gr.6.9.807 · Cited by 463
Construction and characterization of a normalized cDNA library.
Soares, M. B., Bonaldo, M. F., Jelene, P., Su, L., Lawton, L., & Efstratiadis, A. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.20.9228 · Cited by 410
The reeler gene encodes a protein with an EGF–like motif expressed by pioneer neurons
Hirotsune, S., Takahara, T., Sasaki, N., Hirose, K., Yoshiki, A., Ohashi, T., Kusakabe, M., Murakami, Y., Muramatsu, M., Watanabe, S., et al. (1995) · Nature Genetics · doi:10.1038/ng0595-77 · Cited by 345
Self-citationA Drosophila Complementary DNA Resource
Rubin, G. M., Hong, L., Brokstein, P., Evans-Holm, M., Frise, E., Stapleton, M., & Harvey, D. A. (2000) · Science · doi:10.1126/science.287.5461.2222 · Cited by 328
High-Efficiency Full-Length cDNA Cloning by Biotinylated CAP Trapper
Carninci, P., Kvam, C., Kitamura, A., Ohsumi, T., Okazaki, Y., Itoh, M., Kamiya, M., Shibata, K., Sasaki, N., Izawa, M., et al. (1996) · Genomics · doi:10.1006/geno.1996.0567 · Cited by 299
Self-citation
Cited by
Analysis of the mouse transcriptome based on functional annotation of 60,770 full-length cDNAs
The FANTOM Consortium and the RIKEN Genome Exploration Research Group Phase I & II Team* (2002) · Nature · doi:10.1038/nature01266 · Cited by 1,642
The human transcriptome across tissues and individuals
Melé, M., Ferreira, P. G., Reverter, F., DeLuca, D. S., Monlong, J., Sammeth, M., Young, T. R., Goldmann, J. M., Pervouchine, D. D., Sullivan, T. J., et al. (2015) · Science · doi:10.1126/science.aaa0355 · Cited by 1,304
An Abundance of Ubiquitously Expressed Genes Revealed by Tissue Transcriptome Sequence Data
Ramsköld, D., Wang, E. T., Burge, C. B., & Sandberg, R. (2009) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1000598 · Cited by 820
Functional annotation of a full-length mouse cDNA collection
The RIKEN Genome Exploration Research Group Phase II Team and the FANTOM Consortium, The RIKEN Genome Exploration Research Group Phase II Team, Kawai, J., Shinagawa, A., Shibata, K., Yoshino, M., Itoh, M., Ishii, Y., Arakawa, T., Hara, A., et al. (2001) · Nature · doi:10.1038/35055500 · Cited by 665
Self-citationSampath, S. C., Ohi, R., Leismann, O., Salic, A., Pozniakovski, A., & Funabiki, H. (2004) · Cell · doi:10.1016/j.cell.2004.06.026 · Cited by 422
Metadata
- Authors
- Carninci, P., Shibata, Y., Hayatsu, N., Sugahara, Y., Shibata, K., Itoh, M., Konno, H., Okazaki, Y., Muramatsu, M., & Hayashizaki, Y.
- Venue
- Genome Research
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2000-10-01
- Volume
- 10
- Issue
- 10
- Pages
- 1617-1630
- ISSN
- 1088-9051 (ISSN-L)
- 1549-5469
- OpenAlex
- W1998746328
- Cited by
- 290
- References
- 20
- Type
- posted content
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes. https://app.cochat.ai/papers/10.1101/gr.145100
- The paper
- Carninci, P., Shibata, Y., Hayatsu, N., Sugahara, Y., Shibata, K., Itoh, M., Konno, H., Okazaki, Y., Muramatsu, M., & Hayashizaki, Y. (2000). Normalization and Subtraction of Cap-Trapper-Selected cDNAs to Prepare Full-Length cDNA Libraries for Rapid Discovery of New Genes. Genome Research. https://doi.org/10.1101/gr.145100
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
